A5041515937- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Histone Deacetylase Inhibitors Research
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- Microfluidic and Bio-sensing Technologies
- Berberine and alkaloids research
- Protease and Inhibitor Mechanisms
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Silymarin and Mushroom Poisoning
- Innovative Microfluidic and Catalytic Techniques Innovation
- Physiological and biochemical adaptations
- Bone health and treatments
- Neurobiology and Insect Physiology Research
- HER2/EGFR in Cancer Research
- Glycosylation and Glycoproteins Research
- Ginger and Zingiberaceae research
- Electrospun Nanofibers in Biomedical Applications
- Developmental Biology and Gene Regulation
- Mast cells and histamine
- Molecular Communication and Nanonetworks
- Natural Compounds in Disease Treatment
- Colorectal Cancer Treatments and Studies
- Angiogenesis and VEGF in Cancer
Sungkyunkwan University
2012-2024
Seoul National University Bundang Hospital
2019
Suwon Research Institute
2018
Samsung Medical Center
2011-2016
Hanyang University
2014
Seoul National University Hospital
2010
Significance Drug resistance is among the most critical problems in cancer treatment. This proof-of-principle study demonstrates that a combination of vitro microecology and deep sequencing could provide an extremely efficient method to elucidate vivo mechanisms drugs predict if drug likely occur. Remarkably, was seen within 7 d our microfluidic chip, enabling characterization molecular month. would be great value for clinicians selecting slow emergence, choose therapies resistant tumors...
Chemoresistance is one of the major causes therapeutic failure in breast cancer patients. In this study, mechanism chemoresistance human triple-negative (TNBC) cells (MDA-MB-231) induced by doxorubicin (DOX) gradient was investigated. These DOX-resistant showed higher drug efflux rate, increased anchorage-independent growth when cultured suspension, and tumor-forming ability nude mice, compared to wild-type MDA-MB-231 cells. RNA sequencing analysis an increase expression genes involved...
Inflammation is a key regulatory process in cancer development. Prolonged exposure of breast tumor cells to inflammatory cytokines leads epithelial‐mesenchymal transition, which the principal mechanism involved metastasis and invasion. Interleukin (IL)‐1β major cytokine variety tumors. To date, IL‐1β‐induced cell migration invasion has not been fully elucidated. Here, we investigated effect zerumbone (ZER) on cells. The levels IL‐8 matrix metalloproteinase (MMP)‐3 mRNA were analyzed by...
Background/Aims: Berberine (BBR) is an isoquinoline alkaloid and beneficial for the anticancer effect on a variety of human tumor cells. However, BBR's anti-angiogenesis property its clinical potential as inhibitor angiogenesis in breast cancer cells have not been fully elucidated. Here, we investigated BBR TPA-induced VEGF fibronectin (FN) well VEGF-induced FN Methods: The secretion protein was detected by ELISA. Fibronectin mRNA expression analyzed Real-Time PCR western blotting,...
Transforming growth factor-beta (TGF-β) is a multifunctional cytokine that regulates many biological events including cell motility and angiogenesis. Here, we investigated the role of elevated TGF-β2 level in triple negative breast cancer (TNBC) cells inhibitory effect silibinin on action TNBC cells. Breast patients with high expression have poor prognosis. The levels increased significantly compared those non-TNBC In addition, motility-related genes such as fibronectin (FN) matrix...
Overexpression of HER2 correlates with more aggressive tumors and increased resistance to cancer chemotherapy. However, a functional comparison between the HER2high/HER3 HER2low/HER3 dimers on tumor metastasis has not been conducted. Herein we examined regulation mechanism heregulin-β1 (HRG)-induced MMP-1 -9 expression in breast cell lines. Our results showed that basal levels mRNA protein were by HRG treatment. In addition, HRG-induced was significantly decreased MEK1/2 inhibitor, U0126 but...
We describe a method to generate several hundreds of spheroids using microfluidic device with pillars.
Chemoresistance is a major cause of treatment failure in many cancers. However, the life cycle cancer cells as they respond to and survive environmental therapeutic stress understudied. In this study, we utilized microfluidic device induce development doxorubicin-resistant (DOXR) from triple negative breast (TNBC) within 11 days by generating gradients DOX medium. vivo chemoresistant xenograft models, an unbiased genome-wide transcriptome analysis, patient data/tissue analysis all showed...
Acellular nerve grafts (ANGs) are a promising therapeutic for patients with defects caused by injuries. Conventional decellularization methods utilize variety of detergents and enzymes. However, these have disadvantages, such as long processing times the presence that remain on graft. In this study, we aimed to reduce process time minimize risks associated residual replacing them supercritical carbon dioxide (scCO
Background: Cholesterol is a major component of specialized membrane microdomains known as lipid rafts or caveolae, which modulate the fluidity biological membranes.Membrane cholesterol therefore plays an important role in cell signaling and vesicular transport.Objective: In this study, we investigated effects on matrix metalloproteinase-1 (MMP-1) expression human dermal fibroblasts.Methods: MMP-1 mRNA protein were determined by RT-PCR Western blotting, respectively.AP-1 DNA binding activity...
Allotransplantation, performed using an acellular dermal matrix (ADM), plays a significant role in the cultivation of constituted and damaged organs clinical. Herein, we fabricated innovative ADM for allografting derived from decellularized human skin by utilizing supercritical fluid carbon dioxide to eliminate immunogenic components. By histological staining, product demonstrated successful removal cellular constituents without exerting any harmful influence on extracellular matrix. The...
Cyclooxygenase-2 (COX-2) has been reported to be elevated in many cancers, including breast and colorectal resulting accumulation of prostaglandin E<sub>2</sub> the cancer cell environment. In this study, we investigated effect pifithrin (PFT)-α, an inhibitor p53 transactivation, on COX-2 expression fibrosarcoma cells. Our results showed that was dose-dependently increased by PFT-α MDA-MB231 cells with mutant p53. addition, level also normal fibroblasts as well HT1080 wild-type...