A5089422285- Congenital gastrointestinal and neural anomalies
- Circular RNAs in diseases
- Curcumin's Biomedical Applications
- Cancer-related molecular mechanisms research
- Advanced machining processes and optimization
- Advanced Surface Polishing Techniques
- Silymarin and Mushroom Poisoning
- Synthesis and Characterization of Heterocyclic Compounds
- Berberine and alkaloids research
- Infant Nutrition and Health
- MicroRNA in disease regulation
- Synthetic Organic Chemistry Methods
- Synthesis and bioactivity of alkaloids
- Bioactive Compounds and Antitumor Agents
- RNA modifications and cancer
- Computational Drug Discovery Methods
- Synthesis of Organic Compounds
- Natural product bioactivities and synthesis
- Polyamine Metabolism and Applications
- Biological Activity of Diterpenoids and Biflavonoids
- Clinical Nutrition and Gastroenterology
- Chemical Synthesis and Analysis
- Synthesis and Biological Activity
- Ubiquitin and proteasome pathways
- Metal Extraction and Bioleaching
California State University, Fresno
2015-2024
East China Jiaotong University
2024
Second Affiliated Hospital of Nanjing Medical University
2016-2023
Guizhou University
2023
National Marine Environmental Monitoring Center
2022
East China University of Science and Technology
2021-2022
Dalian University of Technology
2022
China National Environmental Monitoring Center
2022
Nanjing Medical University
2016-2020
State Key Laboratory of Reproductive Medicine
2019-2020
// Lei Peng 1, 3, * , Guanglin Chen Zhongxian Zhu Ziyang Shen 3 Chunxia Du Rujin Zang Yang Su Hua Xie Hongxing Li Xiaoqun Xu Yankai Xia 2 Weibing Tang 1 State Key Laboratory of Reproductive Medicine, Institute Toxicology, School Public Health, Nanjing Medical University, China Modern Ministry Education, Department Pediatric Surgery, Children’s Hospital These authors contributed equally to this work Correspondence to: Tang, email: twbcn@163.com Keywords: hirschsprung disease, circular...
Abstract Background Gestational diabetes mellitus (GDM) is a metabolic disease that occurs in pregnant women and increases the risk for development of diabetes. The relationship between GDM meconium microbiota metabolome remains incompletely understood. Methods Four hundred eighteen mothers (147 with 271 normal women) their neonates from Mother Child Study were included this study. Meconium profiled by 16S rRNA gene sequencing. maternal serum examined UPLC-QE. Results Microbial communities...
Purpose.: Micro(mi)RNAs negatively regulate a wide variety of genes through degradation or posttranslational inhibition their target genes. The purpose this study was to investigate the role miR-23a in modulating RPE cell survival and gene expression response oxidative damage. Methods.: level measured macular retinal pigment epithelial (RPE) cells donor eyes with aged-related degeneration (AMD) age-matched normal by using qRT-PCR. Cultured human ARPE-19 were transfected mimic inhibitor. Cell...
Forty-three 1,5-diheteroaryl-1,4-pentadien-3-ones were designed as potential curcumin mimics, structurally featuring a central five-carbon dienone linker and two identical nitrogen-containing aromatic rings. They synthesized using Horner-Wadsworth-Emmons reaction the critical step evaluated for their cytotoxicity antiproliferative activities toward both androgen-insensitive androgen-sensitive prostate cancer cell lines an aggressive cervical line. Most of compounds showed distinctly better...
Abstract Objectives Emerged evidence demonstrates that long non‐coding RNA s (lnc s) may play quintessential regulatory roles in the cellular processes, tumourigenesis and development of disease. Though focally amplified lnc on chromosome 1 ( FAL 1) has been identified to have crucial functions many diseases, its biological mechanism Hirschsprung's disease HSCR ) still remains unknown. Materials methods The expression levels aganglionic tissues matched normal specimens were detected by...
Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause HSCR-related death. Very little known about pathogenesis, prevention, early diagnosis HAEC. Here, we used prospective study investigate enteric microbiome composition at time surgery as predictor for developing postoperative We identified signature containing 21 operational taxonomic units...
Curcumin is a biologically active polyphenol that exists in Indian spice turmeric. It has been reported curcumin exerted anti-inflammatory, anti-oxidant and anti-cancer effects numerous vitro vivo studies. However, it not well-understood the molecular mechanism of for cancer stem cells telomerase colorectal cancer. In this study, compound 19, nitrogen-containing analog, was used to treat human cells. Compound 19 showed greater anti-proliferative activity than while displayed no significant...
Background: Long noncoding RNAs (lncRNAs) have recently emerged as important regulators in a broad spectrum of cellular processes including development and disease.Despite the known engagement AFAP1-AS several human diseases, its biological function Hirschsprung disease (HSCR) remains elusive.Methods: We used qRT-PCR to detect relative expression 64 HSCR bowel tissues matched normal intestinal tissues.The effects on cell proliferation, migration, cycle, apoptosis cytoskeletal organization...
Abstract It has been suggested that circular RNAs play critical roles in natural growth and disease development. Nevertheless, whether the were related Hirschsprung's (HSCR) remains unknown. Thus, we discovered cir‐CCDC66 was downregulated HSCR compared with normal gut tissues. The reduction might inhibit cells’ proliferation migration vitro. Then, found DCX transcript putative competing endogenous RNA. Furthermore, function of as a sponge for miR‐488‐3p to regulate RNA expression...
BackgroundHirschsprung's disease (HSCR) is a common digestive caused by impaired development of neural crest cells. Some studies have revealed the roles microRNA (miRNA) in various diseases. But function miRNA HSCR needs further investigation.MethodsWe adopted qRT-PCR and immunoblot analyses to explore relative expression miR-939 LRSAM1 80 bowel tissues normal tissues. CCK-8 assay, transwell assay flow cytometry were used evaluate overexpression 293T, SK-N-BE(2), SH-SY5Y cell lines. The...
Recently studies reported that long non-coding RNAs (lncRNAs) may take part in a lot of congenital diseases, meanwhile, Hirschsprung's disease (HSCR) is major digestive tract malformation. Nevertheless whether lncRNAs participate the occurrence HSCR and how it contributes to this are still unknown. LOC100507600 was selected from our gene expression microarray data obtained bowel tissues patients negative controls. Subsequently, we used qRT-PCR prove result 64 pairs stenosis Transwell assay,...
In addition to cholesterol-lowering effect, HMG-CoA reductase inhibition by statins has been shown have protective effect in many cells type. The loss of vision retinal degeneration disease associates with oxidative stress and apoptosis pigment epithelium (RPE) cell. This study was designed examine the on oxidant-induced damage human RPE cells. Cultured ARPE-19 (ARPE) were challenged hydrogen peroxide (H(2) O(2) ) plus tumor necrosis factor alpha (TNFα) presence or absence various signaling...
Prostate cancer is the most common diagnosed invasive in American men and second leading cause of cancer-related deaths. Although there are several therapies successful treating early, localized stage prostate cancer, current treatment advanced metastatic castration-resistant remains ineffective due to inevitable progression resistance first-line with docetaxel. The natural product quercetin (3,3',4',5,7-pentahydroxyflavone), a flavonoid compound ubiquitous dietary plants, possesses...
The abnormal PI3K/AKT/mTOR pathway is one of the most common genomic abnormalities in breast cancers including triple-negative cancer (TNBC), and pharmacologic inhibition these aberrations has shown activity TNBC patients. Here, we designed identified a small-molecule Comp34 that suppresses both AKT mTOR protein expression exhibits robust cytotoxicity towards cells but not nontumorigenic normal epithelial cells. Mechanically, long noncoding RNA (lncRNA) AL354740.1-204 (also named as...
Aberrant PP2Cδ inhibits p300-mediated p53 acetylation and activation via the ATM/BRCA1 pathway, leading to cancer progression.