A5066035307- Mycobacterium research and diagnosis
- Ginseng Biological Effects and Applications
- Tuberculosis Research and Epidemiology
- Pharmacological Effects of Natural Compounds
- Infectious Diseases and Mycology
- Plant Pathogenic Bacteria Studies
- Microbial infections and disease research
- Fungal Biology and Applications
- Genomics and Phylogenetic Studies
- Diagnosis and treatment of tuberculosis
- Chemical Reactions and Isotopes
- Diphtheria, Corynebacterium, and Tetanus
- Viral Infections and Vectors
- Medicinal Plant Pharmacodynamics Research
- Analytical Chemistry and Chromatography
- Advanced Chemical Sensor Technologies
- Microbial Community Ecology and Physiology
- Enzyme Structure and Function
- Plant Disease Resistance and Genetics
- Zoonotic diseases and public health
- Veterinary Equine Medical Research
- Microbial bioremediation and biosurfactants
- Vector-borne infectious diseases
- Powdery Mildew Fungal Diseases
- Microbial Metabolism and Applications
Friedrich-Loeffler-Institut
2013-2024
ABSTRACT Mycobacterium avium subsp. paratuberculosis is the etiologic agent of Johne's disease and endemic to national cattle herds many countries. Because very low level genetic heterogeneity this organism, it difficult select a workable procedure for strain differentiation at resolution sufficient investigate epidemiological links between or different ruminant species suggested zoonotic potential M. Crohn's disease. Analysis restriction fragment length polymorphisms (RFLPs) based on...
Multitarget genotyping of the etiologic agent Mycobacterium avium subsp. paratuberculosis is necessary for epidemiological tracing (Johne's disease). The study was undertaken to assess informative value different typing techniques and individual genome markers by investigation M. transmission between wild-living red deer farmed cattle with known shared habitats. Fifty-three type II isolates were differentiated short sequence repeat analysis (SSR; 4 loci), mycobacterial interspersed...
ABSTRACT Haloalkane dehalogenases are enzymes that catalyze the cleavage of carbon-halogen bond by a hydrolytic mechanism. Genomes Mycobacterium tuberculosis and M. bovis contain at least two open reading frames coding for polypeptides showing high sequence similarity with biochemically characterized haloalkane dehalogenases. We describe here cloning dehalogenase genes dmbA dmbB from 5033/66 demonstrate activity their translation products. Both these widely distributed among species complex,...
A total of 50 birds diagnosed with mycobacteriosis were examined for pathomorphological lesions, coinfections, and causative agents. Mycobacterial species identified isolates differentiated using multilocus sequence typing (MLST) mycobacterial interspersed repetitive-unit variable-number tandem-repeat (MIRU-VNTR) analysis. Possible associations between species, findings, bird orders, husbandry conditions evaluated statistically. Mycobacteria isolated from 34 (13 22 Psittaciformes, 12 18...
Mycobacterium avium (M. a.) subsp. paratuberculosis (MAP) - the etiologic agent of Johne's disease affects cattle, sheep and other ruminants worldwide. To decipher phenotypic differences among cattle strains (belonging to MAP-S [Type-I/III] respectively MAP-C [Type-II]) comparative genome analysis needs data from diverse isolates originating different geographic regions world. The current study presents so far best assembled a MAP-S-strain: isolate JIII-386 Germany. One newly sequenced...
Bovine Johne's disease (paratuberculosis), caused by Mycobacterium avium subspecies paratuberculosis, poses a significant economic problem to the beef and dairy industry worldwide. Despite its relevance, however, pathogenesis of is still only partially resolved. Since mycobacterial membrane proteins expressed during infection are likely play an important role in pathogenesis, membrane-enriched fractions, namely mucosa-derived membranes (MDM) culture-derived (CDM), M. subsp. paratuberculosis...
Analysis of volatile organic compounds (VOCs) is a novel approach to accelerate bacterial culture diagnostics Mycobacterium avium subsp. paratuberculosis (MAP). In the present study, cultures fecal and tissue samples from MAP-infected non-suspect dairy cattle goats were explored elucidate effects sample matrix animal species on VOC emissions during cultivation identify early markers for growth. The processed following standard laboratory procedures, tubes incubated different time periods....
Mycobacterium avium (M. a.) subsp. paratuberculosis (MAP) is a worldwide-distributed obligate pathogen in ruminants causing Johne’s disease. Due to lack of complete subtype III genome sequences, there not yet conclusive information about genetic differences between strains cattle (MAP-C, type II) and sheep (MAP-S) type, especially MAP-S subtypes I, III. Here we present the complete, circular MAP-S/type strain JIII-386 (DE) closed by Nanopore-technology its comparison with I Telford (AUS),...
Tapirs seem particularly susceptible to mycobacterial infections, especially tuberculosis caused by M. or bovis. In this case series, we report an infection with the non-tuberculous mycobacteria (NTM) species avium ssp. hominissuis (MAH) in a group of four (2.2) captive lowland tapirs (Tapirus terrestris). Two female showed mild respiratory signs such as coughing and mucous sputum production for several years, one juvenile male tapir had be euthanized due severe dyspnoea, adult only 2010....
Mycobacterium avium subsp. paratuberculosis causes Johne's disease in ruminants and was also detected nonruminant species, including human beings, milk products. We announce here the 4.829-Mb complete genome sequence of cattle-type strain JII-1961 from Germany, which is very similar to strains recovered different continents.
This study investigated the intra- and inter-herd diversity of Mycobacterium avium subsp. paratuberculosis (MAP) isolates from four goat herds in Thuringia (Germany) that were affected by for several years. The main focus was on characterization distribution genotypes among animals environment herd 1. included 196 feces 121 infected goats, various tissues 13 clinically diseased 29 environmental samples 1, additionally, 22 different origin 2 to 4. isolates, sampled between 2018 2022,...
Abstract Background Paratuberculosis, caused by Mycobacterium avium subspecies paratuberculosis (MAP), is a chronic granulomatous enteritis that affects domestic and wild ruminants camelids. The disease has rarely been reported in alpacas Germany. This publication describes epidemiologically independent cases of two Case presentation Two alpacas, 26-year-old female zoo animal (case 1) 2.5-year-old breeding stallion from private owner 2), presented with progressive emaciation, leading to...