A5002571324- Particle physics theoretical and experimental studies
- Quantum Chromodynamics and Particle Interactions
- High-Energy Particle Collisions Research
- Click Chemistry and Applications
- Molecular Sensors and Ion Detection
- Particle Accelerators and Free-Electron Lasers
- Nitric Oxide and Endothelin Effects
- Ubiquitin and proteasome pathways
- Analytical Chemistry and Sensors
- Computational Physics and Python Applications
- RNA and protein synthesis mechanisms
- Superconducting Materials and Applications
- interferon and immune responses
- Indoor Air Quality and Microbial Exposure
- Peptidase Inhibition and Analysis
- Black Holes and Theoretical Physics
- Air Quality and Health Impacts
- Sulfur Compounds in Biology
- Medical Imaging Techniques and Applications
- Antimicrobial Peptides and Activities
- Bacterial Genetics and Biotechnology
- Endoplasmic Reticulum Stress and Disease
- Advanced Proteomics Techniques and Applications
- Adenosine and Purinergic Signaling
- Advanced Chemical Sensor Technologies
Peking University
2011-2025
Shenzhen Bay Laboratory
2022-2024
Guangxi Medical University
2024
China University of Geosciences
2024
First Affiliated Hospital of GuangXi Medical University
2024
The University of Texas Health Science Center at Houston
2021
Peking University Shenzhen Hospital
2020-2021
Rockefeller University
2014-2019
University of Science and Technology of China
2011-2018
Materials Science & Engineering
2018
Over the past years, fluorescent proteins (e.g., green proteins) have been widely utilized to visualize recombinant protein expression and localization in live cells. Although powerful, tags are limited by their relatively large sizes potential perturbation function. Alternatively, site-specific labeling of with small-molecule organic fluorophores using bioorthogonal chemistry may provide a more precise less perturbing method. This approach involves incorporation unnatural amino acids (UAAs)...
A highly efficient and concise synthetic scheme for rhodol fluorophores is developed with palladium-catalyzed amination reaction as the key step. This new route utilized to construct a library, potentially useful design of novel fluorescent probes.
Studying the functions of S-palmitoylated proteins in cells can be challenging due to membrane targeting property and dynamic nature protein S-palmitoylation. New strategies are therefore needed specifically capture complexes cellular membranes for dissecting their vivo. Here we present a bifunctional fatty acid chemical reporter, x-alk-16, which contains an alkyne diazirine, metabolic labeling photo-cross-linking involved mammalian cells. We demonstrate that x-alk-16 metabolically...
Cryptococcus neoformans is an opportunistic yeast that kills over 625,000 people yearly through lethal meningitis. Host phagocytes serve as the first line of defense against this pathogen, but fungal engulfment and subsequent intracellular proliferation also correlate with poor patient outcome. Defining interactions facultative pathogen host key to understanding latter's opposing roles in infection how they contribute latency, dissemination, virulence. We used high-content imaging a human...
l-Lactylation is a recently discovered post-translational modification occurring on histone lysine residues to regulate gene expression. However, the substrate scope of lactylation, especially that in non-histone proteins, remains unknown, largely due limitations current methods for analyzing lactylated proteins. Herein, we report an alkynyl-functionalized bioorthogonal chemical reporter, YnLac, detection and identification protein lactylation mammalian cells. Our in-gel fluorescence...
Long-lived individuals have been extensively studied as a model to investigate the role of gut microbiota in aging, but their fungi remain almost unexplored. Here, we recruited community-dwelling cohort 251 participants (24–108 years, including 47 centenarians) from Guangxi China characterize mycobiome signatures. We found markedly varied during aging and determined predominant factor driving these variations. For long-lived individuals, core taxa, Penicillium Aspergillus, were maintained...
Abstract Acylation of proteins with fatty acids is important for the regulation membrane association, trafficking, subcellular localization, and activity many cellular proteins. While significant progress has been made in our understanding two major forms protein acylation acids, N ‐myristoylation S ‐palmitoylation, studies lysine residues, within proteins, have lagged behind. Demonstrated here use integrative chemical biology approaches to examine human sirtuins as de‐fatty‐acid acylases...
Systematic investigation of various homoallylamines reveals N-p -methoxybenzyl homoallylamine as the optimal 2-aza-Cope reaction moiety for development highly efficient formaldehyde fluorescent probes in vitro and vivo imaging.
Lysine ε-N-L-lactylation is a newly discovered post-translational modification. Herein we present the genetic encoding of ε-N-L-lactyllysine in bacterial and mammalian cells, allowing preparation site-specifically ε-N-L-lactylated recombinant proteins construction fluorescent luminescent probes for detecting delactylases living cells. Using these probes, demonstrate sirtuin 1 as potential delactylase non-histone proteins.
Vibrio species, the Gram-negative bacterial pathogens causing cholera and sepsis, produce multiple secreted virulence factors for infection pathogenesis. Among these is multifunctional-autoprocessing repeats-in-toxin (MARTX) toxin that releases several critical effector domains with distinct functions inside eukaryotic host cells. One such domain, Rho inactivation domain (RID), has been discovered to catalyze long-chain N
<title>Abstract</title> Background Gannan navel orange orchards currently rely heavily on inorganic fertilizers, which have significantly degraded soil quality. Therefore, developing sustainable methods aligned with modern green agriculture is crucial. Plant growth-promoting rhizobacteria (PGPR) are beneficial microorganisms that can promote plant growth and contribute to ecological balance. In-depth research application of PGPR support both agricultural productivity environmental...
We report a measurement of ${D}^{0}\text{\ensuremath{-}}{\overline{D}}^{0}$ mixing parameters and search for indirect $CP$ violation through time-dependent amplitude analysis ${D}^{0}\ensuremath{\rightarrow}{K}_{S}^{0}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}$ decays. The results are based on $921\text{ }\text{ }{\mathrm{fb}}^{\ensuremath{-}1}$ data accumulated with the Belle detector at KEKB asymmetric-energy ${e}^{+}{e}^{\ensuremath{-}}$ collider. Assuming conservation, we...
We analyze the sensitivity of next-generation tonne-scale neutrinoless double-$\ensuremath{\beta}$ decay ($0\ensuremath{\nu}\ensuremath{\beta}\ensuremath{\beta}$) experiments and searches for same-sign di-electrons plus jets at Large Hadron Collider to TeV scale lepton number violating interactions. Taking into account previously unaccounted physics detector backgrounds LHC, renormalization group evolution, long-range contributions $0\ensuremath{\nu}\ensuremath{\beta}\ensuremath{\beta}$...