A5051840800- Zebrafish Biomedical Research Applications
- Sulfur Compounds in Biology
- Coal and Coke Industries Research
- MicroRNA in disease regulation
- Plant Molecular Biology Research
- Telomeres, Telomerase, and Senescence
- Pharmacogenetics and Drug Metabolism
- Nitric Oxide and Endothelin Effects
- Blood Coagulation and Thrombosis Mechanisms
- Coal and Its By-products
- Aquaculture disease management and microbiota
- Neuroinflammation and Neurodegeneration Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Cell death mechanisms and regulation
- Genomics, phytochemicals, and oxidative stress
- Thermochemical Biomass Conversion Processes
- Animal testing and alternatives
- Coal Properties and Utilization
- Drug Transport and Resistance Mechanisms
- Receptor Mechanisms and Signaling
- Cancer therapeutics and mechanisms
- Pregnancy and preeclampsia studies
- Effects and risks of endocrine disrupting chemicals
- Pancreatitis Pathology and Treatment
- Genetics, Aging, and Longevity in Model Organisms
Xi'an International Studies University
2016-2023
Anhui Agricultural University
2023
Kunming Medical University
2022
Sixth Affiliated Hospital of Kunming Medical University
2022
Zhejiang Academy of Medical Sciences
2020-2021
Hangzhou Medical College
2021
Beijing University of Technology
2021
Hunter Genetics
2019-2020
Henan Agricultural University
2009-2019
Shaanxi Normal University
2014-2018
The toxicity mechanism of nanoparticles on vertebrate cardiovascular system is still unclear, especially the low-level exposure. This study was to explore toxic effect and mechanisms low-dose exposure silica (SiNPs) cardiac function in zebrafish embryos via intravenous microinjection. dosage SiNPs based no observed adverse level (NOAEL) malformation assessment embryos. mainly phenotypes induced by were pericardial edema bradycardia but had atrioventricular block. Using o-Dianisidine for...
The zebrafish as an alternative animal model for developmental toxicity testing has been extensively investigated, but its assay protocol was not harmonized yet. This study validated and optimized the previously reported by multiple inter-laboratory studies in United States Europe. In this study, using classical protocol, of 31 ICH-positive compounds, 23 compounds (74.2%) were teratogenic zebrafish, five had false-negative results, three neither nor non-teratogenic according to standard; 14...
The transcription factor NF-E2-related nuclear 2 (Nrf2) regulates expression of genes that protect cells from oxidative damage. Here, we characterized nitric oxide (*NO)-induced Nrf2-Kelch-like ECH-associated protein 1 (Keap1) signaling and its role in counteracting *NO-induced apoptosis human colon cancer HCT116 cells. Nrf2 was localized the cytoplasm control cells; *NO triggered rapid accumulation, transcriptional activation, up-regulation HO-1, NQO1, GCL, but not GST A4 P1 subunits....
Environmental pollution by emerging contaminants, e.g. pharmaceuticals, has become a matter of widespread concern in recent years. We investigated the membrane transport diclofenac and its toxic effects on gene expression development zebrafish embryos. The association with embryos conformed to general partition model at low concentration, coefficient being 0.0033 ml per embryo. At high interaction fitted Freundlich model. Most remained extracellular aqueous solution less than 5% interacting...
Thrombosis is a leading cause of death and the development effective safe therapeutic agents for thrombotic diseases has been proven challenging. In this study, taking advantage transparency larval zebrafish, we developed zebrafish thrombosis model drug screening efficacy assessment. Zebrafish at 2 dpf (days post fertilization) were treated with phenylhydrazine (PHZ) testing 24 h. Tested drugs administered into either by direct soaking or circulation microinjection. Antithrombotic was...
Six new C21 steroidal glycosides, cynotophyllosides A–F (1–6), together with 16 known compounds, were isolated from the roots of Cynanchum otophyllum. The structures compounds elucidated by spectroscopic analysis and chemical methods. three major components, otophylloside F (15), B (17), rostratamine 3-O-β-d-oleandropyranosyl-(1→4)-β-d-cymaropyranosyl-(1→4)-β-d-cymaropyranoside (18), suppressed seizure-like locomotor activity caused pentylenetetrazole in zebrafish. Preliminary...
Abstract Acute lung injury (ALI) and its more severe form, acute respiratory distress syndrome (ARDS), are life-threatening diseases that associated with high mortality rates due to treatment limitations. Neutrophils play key roles in the pathogenesis of ALI/ARDS by promoting inflammation alveolar microenvironment. To date, vivo functional approaches have been limited inaccessibility sacs, which located at anatomical terminal duct mammals. We first characterize swim bladder zebrafish larva,...
Abstract Momordica cochinchinensis (Lour.) Spreng is an indigenous South Asian edible fruit, and seeds of have been used therapeutically in traditional Chinese medicine. Previous studies shown that M. seed (Momordicae Semen) has various pharmaceutical properties such as antioxidant anti‐ulcer effects well contains secondary metabolites with potential anticancer activities triterpenoids saponins. Recent reported water extract ethanol cochinchinensi were tested on mammals using acute toxic...
Toxicity induced by nitric oxide (NO•) has been extensively investigated in many vitro and vivo experimental models. Recently, our laboratories found that both concentration cumulative total dose are critical determinants of cell death caused NO•. Here, we report results studies designed to define thresholds threshold effects for several NO•-induced toxicity cellular responses determine impacts p53 on them. We exposed human lymphoblastoid TK6 cells harboring wild-type isogenic p53-null NH32...
Abstract Abscisic acid (ABA) regulates various developmental processes and stress responses in plants. Protein phosphorylation/dephosphorylation is a central post-translational modification (PTM) ABA signaling. However, the phosphoproteins regulated by under osmotic remain unknown maize. In this study, maize mutant vp5 (deficient biosynthesis) wild-type Vp5 were used to identify leaf stress. Up 4052 phosphopeptides, corresponding 3017 phosphoproteins, identified Multiplex run iTRAQ-based...
This study was designed to explore the therapeutics and mechanisms of a patented marked gastric acid intestine juice-resistant probiotics Bifidobacterium lactis BL-99 ( B . BL-99) on intestinal inflammation functions in zebrafish models. After feeding for 6 hours, fully retained larval tract stayed over 24 hours. promoted motility effectively alleviated aluminum sulfate-induced constipation p < 0.01). Irregular high glucose diet induced adult functional metabolic disorders. fed with...
Nitric oxide (NO • ) is mutagenic and, under appropriate conditions of exposure, also induces apoptosis in many vitro and vivo experimental models. Biochemical cellular mechanisms through which NO are incompletely understood, but involve p53/mitochondria-dependent signaling pathways. In this study, we exposed human lymphoblastoid cells harboring either wild-type (TK6 cells) or mutant p53 (WTK-1 to , delivered by diffusion Silastic tubing. Cells were for 2 h at constant rates 100–533 nM/s,...
Retinoic signaling plays an important role in cell proliferation and differentiation. Disruption of retinoic via excessive or deficient acid can cause teratogenic effects on developing embryos. Similar to acid, many xenobiotic environmental pollutants have been found disrupt through binding eliciting agonistic activity receptors. Currently, studies acid-like compounds aquatic organisms mainly focused teratogenicity few explored their neurobehavioral toxicity. In the present study, authors...
Abstract Furanodiene is a natural terpenoid isolated from Rhizoma Curcumae , well-known Chinese medicinal herb that presents anticancer effects in various types of cancer cell lines. In this study, we have successfully established zebrafish xenografts with 5 human lines; and validated these models anti-cancer drugs used clinically for treating patients. We found was therapeutically effective JF 305 pancreatic cells MCF-7 breast xenotranplanted into zebrafish. showed markedly synergistic...
High-alkali species in coal are notorious for causing ash slagging and fouling incidents. In this paper, four high-alkali coals were individually subject to hydrothermal pretreatment (HTP), within a batch-type autoclave at 300 °C 1 h, the treated analyzed, along with oxygen-containing functional groups determined by Fourier transform infrared spectrometer (FT-IR). Then alkali other components quantified X-ray fluorescence (XRF) evaluating tendency. Apart from this, FactSage was adopted...
Lung cancer is the leading cause of cancer-related death worldwide. Epithelial-mesenchymal transition (EMT) promotes lung progression and metastasis, especially in adenocarcinoma. Sex determining region Y-box protein 5 (SOX5) known to stimulate various cancers. Here, we used immunohistochemical analysis reveal that SOX5 levels were increased 90 adenocarcinoma patients. The high expression non-tumor counterparts correlated with patients' poor prognosis. Inhibiting attenuated metastasis cells,...