A5025294353- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Pesticide Exposure and Toxicity
- Advanced NMR Techniques and Applications
- T-cell and B-cell Immunology
- Insect and Pesticide Research
- Systemic Lupus Erythematosus Research
- Animal Ecology and Behavior Studies
- Ginseng Biological Effects and Applications
- Glycogen Storage Diseases and Myoclonus
- Liver Diseases and Immunity
- Biotin and Related Studies
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- Atomic and Subatomic Physics Research
- Glioma Diagnosis and Treatment
- Pancreatic function and diabetes
- Genetics and Neurodevelopmental Disorders
- Traditional Chinese Medicine Studies
- Parkinson's Disease Mechanisms and Treatments
- Diabetes Management and Research
- Cytokine Signaling Pathways and Interactions
- Neonatal Health and Biochemistry
Florida College
2023-2025
University of Florida
2017-2025
Metabolic imaging produces powerful visual assessments of organ function in vivo. Current techniques can be improved by safely increasing metabolic contrast. The gold standard, 2-[ 18 F]fluorodeoxyglucose-positron emission tomography (FDG-PET) imaging, is limited radioactive exposure and sparse assessment metabolism beyond glucose uptake retention. Deuterium magnetic resonance (DMRI) with [6,6- 2 H ]glucose nonradioactive, achieves tumor contrast, but enriched contrast from deuterated water...
Impaired metabolism is recognized as an important contributor to pathogenicity of T cells in Systemic Lupus Erythematosus (SLE). Over the last two decades, we have acquired significant knowledge about signaling and transcriptomic programs related metabolic rewiring healthy SLE cells. However, our understanding network activity derives largely from studying pathways isolation. Here, argue that enzymatic activities are necessarily coupled through mass energy balance constraints with in-built...
The role of ketones in metabolic health has progressed over the past two decades, moving from what was perceived as a simple byproduct fatty acid oxidation to central player multiplicity disease states. Previous work with hyperpolarized (HP) 13 C shown that ketone production can be detected when using precursors labeled acetyl-CoA at C1 position, often tissues are not normally recognized ketogenic. Here, we assay metabolism HP [2- C]pyruvate perfused mouse liver, classic testbed where...
Metabolic dysfunction-associated steatotic liver disease (MASLD) prevalence is increasing annually and affects over a third of US adults. MASLD can progress to metabolic steatohepatitis (MASH), characterized by severe hepatocyte injury, inflammation, eventual advanced fibrosis or cirrhosis. MASH predicted become the primary cause transplant 2030. Although etiology MASLD/MASH incompletely understood, dysregulated fatty acid oxidation implicated in pathogenesis. Here, we develop method for...
Abstract BACKGROUND Diffuse Midline Glioma (DMG) is an inoperable pediatric brain tumor with insufficient treatment options. Our group has developed a novel modality for this disease and others, using mRNA vaccines consisting of derived antigens in unique lipid-nanoparticle (NP). METHODS We have treated mice, canines, human patients primary tumors to define local immunotherapuetic response. our neonatally implanted DMG model NPs generated from total-tumor beginning day 31 used MRI, MR...
Alzheimer’s Disease (AD) is characterized by increased inflammation and glucose utilization as detected FDG-PET. Using a new deuterium MRI method based on [D7]glucose tracer, we can image the brain of mouse model AD with in plane resolution approaching 1 mm. Mass spectrometry imaging same brains shows characteristic changes protein associated glycans that correlate well immunohistochemical staining for inflammation. We believe this research pipeline provide powerful insights into pathophysiology.
Transcriptome data were collected in rat dopamine cells exposed to fipronil for 24 h using microarray analysis. Fipronil is a phenylpyrazole pesticide that acts inhibit gamma-aminobutyric acid (GABA), blocking inhibitory synaptic transmission the central nervous system. subjected pathway analysis and subnetwork enrichment We report 25 µM altered transcriptional networks dopamine-synthesizing are associated with Alzheimer's Disease, Huntington Schizophrenia. Data revealed nerve fibre...
Abstract In a clinical setting, ex vivo perfusions are routinely used to maintain and assess organ viability prior transplants. Organ also model system examine metabolic flux while retaining the local physiological structure, with significant success using hyperpolarized (HP) 13 C NMR in this context. We use novel exocrine pancreas perfusion technique via common bile duct acinar cell metabolism HP [1‐ C]pyruvate. The component of produces digestive enzymes through ductal is often neglected...
Abstract Nonalcoholic fatty liver disease (NAFLD) prevalence is increasing annually and affects over a third of U.S. adults. NAFLD can progress to nonalcoholic steatohepatitis (NASH), characterized by severe inflammation fibrosis. NASH predicted become the primary cause transplant 2030. Although etiology NAFLD/NASH incompletely understood, dysregulated acid oxidation implicated in pathogenesis. Here, we developed method for estimating hepatic β-oxidation from metabolism [D 15 ]octanoate...
Nonalcoholic fatty liver disease (NAFLD) prevalence is increasing annually and affects over a third of U.S. adults. NAFLD can progress to nonalcoholic steatohepatitis (NASH), characterized by severe inflammation fibrosis. NASH predicted become the primary cause transplant 2030. Although etiology NAFLD/NASH incompletely understood, dysregulated acid oxidation implicated in pathogenesis. Here, we developed method for estimating hepatic b-oxidation from metabolism [D15]octanoate deuterated...
The pancreas functions as both an endocrine and exocrine gland. component consists of the islets Langerhans, which contain several pancreatic cell types that produce hormones. In islets, α-cells glucagon β-cells insulin, serve to regulate glucose metabolism. tissue produces digestive enzymes such chymotrypsin is connected through ductal system. Type I Diabetes (T1D) autoimmune disease in immune cells attack β-cells, resulting producing little no insulin. Although vast majority research has...
Non-alcoholic fatty liver disease (NAFLD) is characterized by >5% hepatic fat mass. As one of the most common diseases, with a prevalence roughly 24% in U.S. adult population, NAFLD related morbidities account for striking ~300 billion dollars year healthcare costs alone [Shetty, A., & Syn, W. K. Federal practitioner: health care professionals VA, DoD, and PHS, (2019), 36(1), 14-19]. NAFLD, both humans murine models disease, has been associated increased rates β-oxidation reactive oxygen...