A5057714553- Cancer, Hypoxia, and Metabolism
- Neuroblastoma Research and Treatments
- Ubiquitin and proteasome pathways
- Genetics and Neurodevelopmental Disorders
- Glycogen Storage Diseases and Myoclonus
- Metabolomics and Mass Spectrometry Studies
- Lysosomal Storage Disorders Research
- Mass Spectrometry Techniques and Applications
- Cell Image Analysis Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Proteomics Techniques and Applications
- Nuclear Receptors and Signaling
- Neonatal and fetal brain pathology
- Signaling Pathways in Disease
- Electrolyte and hormonal disorders
- Single-cell and spatial transcriptomics
- Neurological Disease Mechanisms and Treatments
- Immune cells in cancer
- Chaos-based Image/Signal Encryption
- Mitochondrial Function and Pathology
- Coenzyme Q10 studies and effects
University of Florida
2023-2025
University of Kentucky
2024
Florida College
2024
Microglia undergo two-stage activation in neurodegenerative diseases, known as disease-associated microglia (DAM). TREM2 mediates the DAM2 stage transition, but what regulates first DAM1 transition is unknown. We report that glucose dyshomeostasis inhibits and PKM2 plays a role. As tumors, was aberrantly elevated both male female human AD brains, unlike it expressed active tetramers, well among + surrounding plaques 5XFAD mice. snRNAseq analyses of without Pkm2 mice revealed significant...
Abstract Background: Spatial integration of metabolic pathways with single cell-level transcriptomic data in human tumor and normal tissue may elucidate mechanisms supporting tumorigenesis for therapeutic targeting. The suppressive adenosine signaling pathway is an emerging potential target combination radiotherapy. This study aims to create situ map the non-small cell lung cancer (NSCLC) adjacent tissue. We seek define how distinct cellular compartments cooperate contribute within...
MYCN-amplification is a genetic hallmark of ~40% high-risk neuroblastomas (NBs). Altered glycosylation common feature adult cancer progression, but little known about how signatures such as alter profiles. Herein, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) revealed increased core fucosylated glycan abundance within neuroblast-rich regions human MYCN-amplified NB tumors. GDP-mannose 4,6-dehydratase (GMDS) responsible for the first-committed and...
ABSTRACT Metabolites, lipids, and glycans are fundamental biomolecules involved in complex biological systems. They metabolically channeled through a myriad of pathways molecular processes that define the physiology pathology an organism. Here, we present blueprint for simultaneous analysis spatial metabolome, lipidome, glycome from single tissue section using mass spectrometry imaging. Complimenting original experimental protocol, our workflow includes computational framework called Spatial...
Abstract Introduction: MYCN-amplification (MYCN-amp) is a genetic hallmark of ~40% high-risk neuroblastomas (NBs). N-MYC an oncogenic transcription factor and master regulator metabolism. Glycosylation major post-translational modification that critical for cancer progression, spread, immune evasion. How signatures such as MYCN-amp alter glycosylation profiles unknown. Herein, we utilized matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to define the N-linked...
Pompe disease is due to mutations in the acid a-glucosidase (GAA) gene. GAA protein degrades lysosomal glycogen and dysfunctional or absent leads cellular accumulation. In recent years, it has become clear that accumulation central nervous system (CNS) needs be targeted disease. However, standard of care — intravenous enzyme replacement therapy using recombinant does not effectively target CNS. Here, we investigated ability neonatal AAV-GAA neuronal ( Gaa −/− ) rat. On post-natal day one,...
Abstract Parvalbumin-positive interneurons (PV-INs) regulate neuronal and circuit activity, their dysfunction is observed across neurological conditions, including traumatic brain injury (TBI), epilepsy, Alzheimer’s disease, schizophrenia. PV-INs are particularly vulnerable to cell loss, potentially due increased metabolic demands arising from uniquely high level of electrical which render them susceptible pressure. Here, we use single-nucleus RNA-sequencing (snRNAseq) data a rodent model...
<title>Abstract</title> <italic>MYCN-</italic>amplification is a genetic hallmark of ~ 40% high-risk neuroblastomas (NBs). Altered glycosylation common feature adult cancer progression, but little known about how signatures such as <italic>MYCN</italic>-amplification alter profiles. Herein, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) revealed increased core fucosylated glycan abundance within human <italic>MYCN-</italic>amplified NB tumors. GDP-mannose...
Absence of functional acid-α-glucosidase (GAA) leads to early-onset Pompe disease with cardiorespiratory and neuromuscular failure. A novel rat model (
High-resolution spatial imaging is transforming our understanding of foundational biology. Spatial metabolomics an emerging field that enables the dissection complex metabolic landscape and heterogeneity from a thin tissue section. Currently, metabolism highlights remarkable complexity in two-dimensional space poised to be extended into three-dimensional world Here, we introduce MetaVision3D, novel pipeline driven by computer vision techniques for transformation serial 2D MALDI mass...