The current study aims to evaluate the potential roles of taking probiotics postoperatively in attenuating gastrointestinal complications and disturbed gut microbiota colorectal cancer (CRC) patients undergoing chemotherapy. One hundred eligible CRC who were treated with radical surgery needed receive chemotherapy recruited. Half them randomly assigned Probio group take a probiotic combination from post-operation end first chemotherapeutic course. other half placebo instead classified as...

Background Gastrectomy is the most effective treatment to improve clinical survival rate of patients with gastric cancer. However, pathophysiological changes caused by gastrectomy have seriously affected postoperative recovery. Methods In present trial, Ataining (containing C. butyricum , CGMCC0313.1) was applied in after investigate effect on early recovery monitoring inflammatory immune response blood indicators, detecting gut microbiota high-throughput sequencing, and analyzing...

Gastrectomy has been widely used for the treatment of gastric cancer, and severity physiological microbial disorders greatly harmed health patients.In present study, a probiotic combination containing Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis Bacillus cereus was to reduce induced by gastrectomy via monitoring blood index diversity using high-throughput sequencing.Our results indicated that had significantly reduced inflammation indexes (leukocyte) (p<0.05),...

The diamondback moth, Plutella xylostella, is a cosmopolitan pest and the first species to develop field resistance toxins from gram-positive bacterium Bacillus thuringiensis (Bt). Although previous work has suggested that mutations of ATP-binding cassette transporter subfamily C2 (ABCC2) or C3 (ABCC3) genes can confer Cry1Ac resistance, here we reveal P. xylostella requires combined in both PxABCC2 PxABCC3 achieve high-level rather than simply mutation either gene. We identified natural...

Recently, long non-coding RNA (lncRNA) MIAT has been demonstrated as an oncogenic gene in several types of cancer. However, the role and mechanism colorectal cancer (CRC) have not investigated.Real-time PCR was used to measure expression CRC tissues cells. Small interfering specific for (si-MIAT) down-regulate The interaction miR-132 measured by pull-down assay. effect si-MIAT on cells apoptosis metastasis were flow cytometry assay, invasion migration respectively.In present study, we found...

Abstract Understanding the genetic basis of climatic adaptation is essential for predicting species’ responses to climate change. However, intraspecific variation these arising from local remains ambiguous most species. Here, we analyze genomic data diamondback moth ( Plutella xylostella ) collected 75 sites spanning six continents reveal that climate-associated adaptive exhibits a roughly latitudinal pattern. By developing an eco-genetic index combines and physiological responses, predict...

Dysregulated glycolysis is one of the mechanisms employed by cancer cells to facilitate growth and metastasis. Here we aimed characterize PPFIA4 gene, as a glycolysis-related oncogene in promoting proliferation migration colon cells. Using bioinformatical tools including The Cancer Genome Atlas (TCGA) Gene Expression Profiling Interactive Analysis (GEPIA), found that expression methylation levels were higher tissues different stages than normal tissues. Higher level was also positively...

Human plasma and serum samples, including protein peptide biomarkers, are subjected to preanalytical variations instability caused by intrinsic proteases. In this study, we directly investigated the stability of biomarkers spiking an isotopically labeled into human samples then monitoring its time-dependent change. Fibrinogen A (FPA) was used as a model substrate, degradation in conventional either with citrate, heparin, or EDTA anticoagulant, plus protease inhibitors (inhibited plasma),...

Infertility is one of the most common reproductive system diseases, and no effective method available for its treatment. Although in vitro fertilization (IVF) has been widely used to enhance clinical pregnancy outcome infertility, unsatisfied rate with unknown reasons obtained. To identify possible cause IVF failure, 555 patients were enrolled present study determine their relevant characteristics vaginal microbiota. Our results indicated that age endometrium thickness significantly affected...

Notch3 and pS6 play important roles in tumor angiogenesis. To assess the expression of Chinese ovarian epithelial cancer patients, a ten-year follow-up study was performed tissues from 120 specimens human cancer, 30 benign tumors, samples healthy ovaries by immunohistochemistry. The results indicate that higher than normal ovary (p < 0.01). In tissues, were negatively associated with age > 0.05) but positively clinical stage, pathological grading, histologic type, lymph node metastasis,...

Background: Bowel preparation is necessary for successful colonoscopy, while it can seriously affect intestinal microbial composition and damage the mucosal barriers in humans. Methods: To figure out whether probiotics sustain homeostasis guard people's health, probiotic drug of Bifidobacterium Tetragenous viable Bacteria Tablets (P group, n=16) or placebo (C was used volunteers receiving bowel preparation, high-throughput sequencing method applied to monitor their changes. Results: The...

Endometrial injury is the main cause of intrauterine adhesions (IUA), and there currently no effective prevention treatment. Immune cells play an important role in damage repair by sensing change microenvironment. Exogenous CXCL12 can promote tissue regeneration recruiting immune cells, but its effect possible mechanism on endometrial have not been reported. In present study, we constructed engineered a Lactobacillus crispatus strain transforming pMG36e plasmid carrying gene into bacterium,...

Abstract Background Intrauterine adhesion (IUA) is a frequent acquired endometrial condition, for which there no effective preventive or treatment. Previous studies have found that vaginal microbiota dysregulation closely related to fibrosis and IUA. Therefore, we wondered whether restoration of by administration L. crispatus could prevent ameliorate Results First, created mechanically injured mouse model IUA restored the mice’s addition convolvulus. The observations suggested intrauterine...

Six reversed-phase columns from different manufacturers were characterized in terms of adsorbent geometry (e.g., pore volume, surface area, column void and interparticle volume). Measurement the area chemically modified silica-based adsorbents is discussed together with methods for determination amount column. The behavior nearly ideal chromatographic systems was studied. Retention factors alkylbenzenes acetonitrile/water methanol/water compared surface-specific retention factors....

Monochamus alternatus Hope is the main vector in China of Pine Wilt Disease caused by pine wood nematode Bursaphelenchus xylophilus. Although chemical control traditionally used to prevent wilt disease, new strategies based biological are promising ways for management disease. However, there no deep sequence analysis that describes transcriptome and information available about gene function this insect vector. We next generation sequencing technology whole fourth instar larva successfully...

Opsins are crucial for animal vision. The identity and function of opsins in Plutella xylostella remain unknown. aim the research is to confirm which opsin gene(s) contribute phototaxis P. xylostella.LW-opsin, BL-opsin UV-opsin, were identified genome. LW-opsin was more highly expressed than other two genes, all three genes specifically head. Three strains, LW-13 with a 13-bp deletion LW-opsin, BL + 2 2-bp insertion BL-opsin, UV-29 5-bp 34-bp established from strain G88 using CRISPR/Cas9...

Monoclonal antibody technology plays a vital role in biomedical and immunotherapy, which greatly promotes the study of structure function genes proteins. To date, monoclonal antibodies have gone through four stages: murine antibody, chimeric humanised fully human antibody; thousands been used fields biology medicine, playing special pathogenesis, diagnosis treatment disease. In this review, we compare advantages disadvantages hybridoma technology, phage display ribosome transgenic mouse...

Gastrectomy can improve the clinical survival rate of gastric cancer patients, while metabolic disorders caused by gastrectomy, seriously affect postoperative recovery. In this study, probiotics (including Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis and Bacillus cereus) were used before gastrectomy to explore effect on patients. Our outcomes revealed could alleviate inflammation (Leukocyte; P < 0.05), disorder (fasting glucose; fasting insulin; HOMA-IR; reduce...

Juvenile hormone binding protein (JHBP) is a key regulator of JH signaling, and crosstalk between 20-hydroxyecdysone (20E) can activate fine-tune the mitogen-activated kinase cascade, leading to resistance insecticidal proteins from Bacillis thuringiensis (Bt). However, involvement JHBP in Bt Cry1Ac Plutella xylostella remains unclear. Here, we cloned full-length cDNA encoding JHBP, quantitative real-time PCR (qPCR) analysis showed that expression PxJHBP gene midgut Cry1Ac-susceptible strain...

ABSTRACT Growing evidence have indicated the crucial role of intratumor microbiome in a variety solid tumor. However, intratumoral gynecological malignancies is largely unknown. In present study, total 90 Han patients, including 30 patients with cancer cervix, ovary, and endometrium each were enrolled, composition was assessed by 16S rDNA amplicon high throughput sequencing. We found that diversity metabolic potential all three types very similar. Furthermore, shared few taxa collectively...