A5045730521- Microbial Community Ecology and Physiology
- Wastewater Treatment and Nitrogen Removal
- Methane Hydrates and Related Phenomena
- Genomics and Phylogenetic Studies
- Microbial bioremediation and biosurfactants
- Microbial Fuel Cells and Bioremediation
- Soil Carbon and Nitrogen Dynamics
- Gut microbiota and health
- Constructed Wetlands for Wastewater Treatment
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial metabolism and enzyme function
- Pesticide and Herbicide Environmental Studies
- Protist diversity and phylogeny
- Plant-Microbe Interactions and Immunity
- Mycorrhizal Fungi and Plant Interactions
- Water Treatment and Disinfection
- Anaerobic Digestion and Biogas Production
- Fire effects on ecosystems
- Toxic Organic Pollutants Impact
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Biocrusts and Microbial Ecology
- Peatlands and Wetlands Ecology
- Forest Ecology and Biodiversity Studies
- Building materials and conservation
Nanjing Agricultural University
2009-2024
Ministry of Agriculture and Rural Affairs
2022-2024
Huazhong Agricultural University
2023
Chinese Academy of Sciences
2011-2021
Institute of Soil Science
2011-2021
University of Vienna
2019
Rice paddy fields are characterized by regular flooding and nitrogen fertilization, but the functional importance of aerobic ammonia oxidizers nitrite under unique agricultural management is poorly understood. In this study, we report differential contributions ammonia-oxidizing archaea (AOA), bacteria (AOB) nitrite-oxidizing (NOB) to nitrification in four soils from different geographic regions (Zi-Yang (ZY), Jiang-Du (JD), Lei-Zhou (LZ) Jia-Xing (JX)) that representative rice ecosystems...
A novel esterase gene, pytH, encoding a pyrethroid-hydrolyzing carboxylesterase was cloned from Sphingobium sp. strain JZ-1. The gene contained an open reading frame of 840 bp. Sequence identity searches revealed that the deduced enzyme shared highest similarity with many alpha/beta-hydrolase fold proteins (20 to 24% identities). PytH expressed in Escherichia coli BL21 and purified using Ni-nitrilotriacetic acid affinity chromatography. It monomeric structure molecular mass approximately 31...
Long‐term field fertilization experiments may provide useful insight into the relative contributions of NH 3 –oxidizing bacteria (AOB) and archaea (AOA) to soil nitrification. In this study, abundance composition AOB AOA were investigated in bulk from paddy plots (4 by 5 m) that received no (CK), 180 kg urea N ha −1 yr (NPK), or plus 4500 rice ( Oryza sativa L.) straw (NPK/OM) since 1988 (each with three replicated plots). Our results clearly indicate long‐term (22 yr) significantly altered...
Abstract Ammonia-oxidizing archaea (AOA) are among the most abundant and ubiquitous microorganisms in ocean, exerting primary control on nitrification nitrogen oxides emission. Although united by a common physiology of chemoautotrophic growth ammonia, corresponding high genomic habitat variability suggests tremendous adaptive capacity. Here, we compared 44 diverse AOA genomes, 37 from species cultivated samples collected across geographic locations seven assembled metagenomic sequences...
Complete ammonia oxidizers (comammox), able to individually oxidize nitrate, are considered play a significant role in the global nitrogen cycle. However, distribution of comammox Nitrospira estuarine tidal flat wetland and environmental drivers affecting their abundance diversity remain unknown. Here, we present large-scale investigation on geographical along wetlands China, where were successfully detected 9 16 sampling sites. The ranged from 4.15 × 105 6.67 106 copies/g, 2.21- 5.44-folds...
Abstract Thaumarchaeota are responsible for a significant fraction of ammonia oxidation in the oceans and soils that range from alkaline to acidic. However, adaptive mechanisms underpinning their habitat expansion remain poorly understood. Here we show into acidic high pressures hadopelagic zone is tightly linked acquisition variant energy-yielding ATPases via horizontal transfer. Whereas ATPase genealogy neutrophilic congruent with organismal inferred concatenated conserved proteins, common...
Phytopathogenic fungi threaten global food security but the ecological drivers of their diversity and biogeography remain unknown. Here, we construct analyse a atlas potential phytopathogenic from 20,312 samples across all continents major oceanic island regions, eleven land cover types, twelve habitat types. We show peak in mid-latitude contrast to latitudinal gradients observed aboveground organisms. Our study identifies climate as an important driver distribution fungi, our models suggest...
Abstract Ammonia-oxidizing archaea (AOA) are among the most ubiquitous and abundant on Earth, widely distributed in marine, terrestrial, geothermal ecosystems. However, genomic diversity, biogeography, evolutionary process of AOA populations subsurface environments vastly understudied compared to those marine soil systems. Here, we report a novel order Candidatus (Ca.) Nitrosomirales which forms sister lineage thermophilic Ca. Nitrosocaldales. Metagenomic 16S rRNA gene-read mapping...
Bacteria and archaea sustain subsurface cave ecosystems by dominating primary production fueling biogeochemical cyclings, despite the permanent darkness shortage of nutrients. However, heterogeneity underlying mechanism microbial diversity in caves, particular those well connect to surface environment are largely unexplored. In this study, we examined bacterial abundance composition Jinjia Cave, a small shallow limestone located on western Loess Plateau China, enumerating pyrosequencing...
ABSTRACT Both bacteria and archaea potentially contribute to ammonia oxidation, but their roles in freshwater sediments are still poorly understood. Seasonal differences the relative activities of these groups might exist, since cultivated archaeal oxidizers have higher temperature optima than bacterial counterparts. In this study, sediment collected from eutrophic Lake Taihu (China) was incubated at different temperatures (4°C, 15°C, 25°C, 37°C) for up 8 weeks. We examined active microcosms...
Abstract. Pure culture studies have demonstrated that methanotrophs and ammonia oxidizers can both carry out the oxidation of methane ammonia. However, expected interactions resulting from these similarities are poorly understood, especially in complex, natural environments. Using DNA-based stable isotope probing pyrosequencing 16S rRNA functional genes, we report on biogeochemical molecular evidence for growth stimulation methanotrophic communities by ammonium fertilization, modulates...
Abstract Plastic pollution and antibiotic resistance are two emerging environmental human health crises today. Although it was revealed that microplastics can serve as vectors for the dissemination of resistance, is still unclear how nanoplastics influence horizontal transfer genes (ARGs). Herein, we firstly compared effect polystyrene (PS) micro/nanoplastics on transformation plasmid‐borne ARG, using a model consisting plasmid pUC19 ( amp R ) Escherichia coli DH5α (recipient). Due to its...
ABSTRACT Halophyte-based remediation emerges as a novel strategy for ameliorating saline soils, offering sustainable alternative to conventional leaching methods. While bioremediation is recognized its ability energize soil fertility and structure, the complex interplays among plant traits, functions, microbial diversity remain greatly unknown. Here, we conducted 5-year field experiment involving continuous cultivation of annual halophyte Suaeda salsa in soils explore their relationships...
All cultivated ammonia-oxidizing archaea (AOA) within the Nitrososphaera cluster (former soil group 1.1b) are neutrophilic. Molecular surveys also indicate existence of Nitrososphaera-like phylotypes in acidic soil, but their ecological roles poorly understood. In this study, we present molecular evidence for chemolithoautotrophic growth AOA an with pH 4.92 using DNA-based stable isotope probing (SIP). Soil microcosm incubations demonstrated that nitrification was stimulated by urea...