A5043422428- Fungal Infections and Studies
- Immune Response and Inflammation
- Antifungal resistance and susceptibility
- Immune Cell Function and Interaction
- Antimicrobial Peptides and Activities
- Cytomegalovirus and herpesvirus research
- Mycobacterium research and diagnosis
- Wound Healing and Treatments
- Pediatric health and respiratory diseases
- Neutropenia and Cancer Infections
- Influenza Virus Research Studies
- Nail Diseases and Treatments
- Liver Diseases and Immunity
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Escherichia coli research studies
- Gold and Silver Nanoparticles Synthesis and Applications
- Pharmaceutical studies and practices
- Helicobacter pylori-related gastroenterology studies
- Nasal Surgery and Airway Studies
- Oral microbiology and periodontitis research
- Nanopore and Nanochannel Transport Studies
- Psoriasis: Treatment and Pathogenesis
- Inflammasome and immune disorders
- Inflammation biomarkers and pathways
- Asthma and respiratory diseases
Tohoku University
2011-2024
Niigata University
2017-2023
Shikoku Medical Center for Children and Adults
2023
ABSTRACT Dectin-2 is a C-type lectin receptor that recognizes high mannose polysaccharides. Cryptococcus neoformans , yeast-form fungal pathogen, rich in polysaccharides its cell wall and capsule. In the present study, we analyzed role of host defense against C. infection. gene-disrupted (knockout) (Dectin-2KO) mice, clearance this fungus inflammatory response, as shown by histological analysis accumulation leukocytes infected lungs, were comparable to those wild-type (WT) mice. The...
Caspase recruitment domain-containing protein 9 (CARD9) is an adaptor molecule signal that critical for NF-κB activation and triggered through C-type lectin receptors (CLRs), which are pattern recognition recognize carbohydrate structures. Previous studies have reported Cryptococcus neoformans, a fungal pathogen causes meningoencephalitis in AIDS patients, recognized some CLRs, such as mannose or DC-SIGN. However, the role of CARD9 host defense against cryptococcal infection remains to be...
Abstract The wound‐healing process consists of the inflammation, proliferation, and remodeling phases. In chronic wounds, inflammation phase is prolonged with persistent neutrophil infiltration. inflammatory response critically regulated by cytokines chemokines that are secreted from various immune cells. Recently, we showed skin wound healing was delayed impaired under conditions lacking invariant natural killer T (iNKT) cells, an innate lymphocyte potent immuno‐regulatory activity. present...
Acute respiratory distress syndrome (ARDS) is accompanied by severe lung inflammation induced various diseases. Despite the severity of symptoms, therapeutic strategies have been ineffective. High mobility group box 1 (HMGB1), which was identified originally as a DNA binding protein, has proposed mediator acute injury. In addition to its anti-coagulant activity, recombinant thrombomodulin (rTM) possesses an ability suppress inflammatory response through neutralizing HMGB1. T regulatory...
In our study, water dispersible magnetite (Fe3O4) nanoparticles were continuously synthesized in under high temperature and pressure the presence of 3,4-dihydroxyhydrocinnamic acid (DHCA) by using a tubular flow reactor. The prepared Fe3O4 well dispersed because surfaces fully covered DHCA molecules –COOH groups exposed to surrounding water. Cytokines such as IL-12 TNF-α not produced from dendritic cells mice co-incubation with Fe3O4. This indicates that had no immune stimulating property...
Type I interferons (IFNs) are secreted by many cell types upon stimulation via pattern recognition receptors and bind to IFN-α/β receptor (IFNAR), which is composed of IFNAR1 IFNAR2. Although type IFNs well known as anti-viral cytokines, limited information available on their role during fungal infection. In the present study, we addressed this issue examining effect defects host defense response Cryptococcus neoformans. IFNAR1KO mice, number live colonies was lower immune mediated not only...
IL-17A is a proinflammatory cytokine produced by many types of innate immune cells and Th17 involved in the elimination extracellularly growing microorganisms, yet role this host defense against intracellularly microorganisms not well known. Cryptococcus deneoformans an opportunistic intracellular growth fungal pathogen that frequently causes fatal meningoencephalitis patients with impaired responses. In current study, we analyzed C. infection. was quickly γδT at phase infected lungs....
ABSTRACT Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningoencephalitis in immunocompromised patients. Recently, we reported Toll-like receptor 9 (TLR9) involved host defense against C. : specifically, it detects the pathogen's DNA. In present study, aimed to elucidate mechanisms underlying TLR9-mediated activation of innate immune responses by using URA5 gene, which encodes a virulent component this pathogen. A PCR-amplified 345-bp gene fragment induced...
Cryptococcus neoformans is rich in polysaccharides of the cell wall and capsule. Dectin-2 recognizes high-mannose plays a central role immune response to fungal pathogens. Previously, we demonstrated was involved activation dendritic cells upon stimulation with C. neoformans, suggesting existence ligand recognized by Dectin-2. In present study, examined structures contributing Dectin-2-mediated cells. NFAT-GFP reporter assay reported expressing Dectin-2, lysates, but not whole yeast cells,...
Cryptococcus deneoformans is an opportunistic fungal pathogen that frequently causes fatal meningoencephalitis in patients with impaired cell-mediated immune responses such as AIDS. Caspase-associated recruitment domain 9 (CARD9) plays a critical role the host defense against cryptococcal infection, suggesting involvement of one or more C-type lectin receptors (CLRs). In present study, we analyzed macrophage-inducible (Mincle), CLRs, C. infection. Mincle expression lungs wild-type (WT) mice...
Abstract The inflammatory response after skin injury involves the secretion of a variety cytokines and growth factors that are necessary for tissue repair. Caspase recruitment domain‐containing protein 9 ( CARD 9) is an essential signalling adaptor molecule NF ‐κB activation upon triggering through C‐type lectin receptors CLR s), which expressed in macrophages dendritic cells. However, role responses at wound site has not been elucidated. In this study, we analysed healing process wounds....
Chronic infections are considered one of the most severe problems in skin wounds, and bacteria present over 90% chronic wounds. Pseudomonas aeruginosa is frequently isolated from wounds thought to be a cause delayed wound healing. Invariant natural killer T (iNKT) cells, unique lymphocytes with potent regulatory ability various inflammatory responses, accelerate healing process. In study, we investigated contribution iNKT cells host defense against P. inoculation at sites. We analyzed...
Cryptococcus deneoformans is an opportunistic fungal pathogen that infects the lungs via airborne transmission and frequently causes fatal meningoencephalitis. Claudins (Cldns), a family of proteins with 27 members found in mammals, form tight junctions within epithelial cell sheets. Cldn-4 18 are highly expressed airway tissues, yet roles these claudins respiratory infections have not been clarified. In present study, we analyzed lung-specific Cldn-18 (luCldn-18) host defense against C....
DNA from Cryptococcus neoformans activates bone marrow-derived dendritic cells (BM-DCs) in a TLR9-dependent manner. In this study, we examined the effect of culture supernatants C. on activation BM-DCs caused by its own DNA. suppressed IL-12p40, IL-6 production and CD40 expression stimulated with DNA, but not CpG-ODN Candida albicans, Saccharomyces cerevisiae or Escherichia coli. confocal microscopic analysis, was colocalized LAMP-1, late endosomal marker, TLR9. The did show any apparent...
Streptococcus pneumoniae is a major causative bacterium of community-acquired pneumonia. Dendritic cell-associated C-type lectin-2 (dectin-2), one the lectin receptors (CLRs), was previously reported to play pivotal role in host defense against pneumococcal infection through regulating phagocytosis by neutrophils while not being involved neutrophil accumulation. In present study, elucidate possible contribution other CLRs accumulation, we examined caspase recruitment domain-containing...
Antigen-presenting cells express pattern recognition receptors (PRRs), which sense pathogen-associated molecular patterns from microorganisms and lead to the induction of inflammatory responses. C-type lectin (CLRs), representative PRRs, bind microbial polysaccharides, among Dectin-2 Mincle recognize mannose-containing polysaccharides. Because influenza virus (IFV) hemagglutinin (HA) is rich in mannose or may contribute HA. In this study, we addressed possible involvement viral initiation...
is a yeast-type fungus that causes fatal meningoencephalitis in immunocompromised patients and evades phagocytic cell elimination through an escape mechanism. Memory T (Tm) cells play central role preventing the reactivation of this fungal pathogen. Among these cells, tissue-resident memory (T
<b><i>Introduction:</i></b> The enhanced type 2 helper (Th2) immune response is responsible for the pathogenesis of allergic asthma. To suppress Th2 response, activation Th1 has been an alternative strategy anti-asthma therapy. In this context, effective Th1-inducing adjuvants that inhibit development asthma but do not flare side effects primary agent are required in clinical treatment and preventive medicine. <b><i>Objective:</i></b> study, we...
2016 International Conference on Solid State Devices and Materials,Reconstitution of Human Ion Channels into Solvent-Free Lipid Bilayers in MicroFabricated Silicon Chips: Accelerated Vesicle Fusion via Centrifugation