A5038533212- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Nanoplatforms for cancer theranostics
- IL-33, ST2, and ILC Pathways
- Virus-based gene therapy research
- Cancer Cells and Metastasis
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Research and Treatments
- Estrogen and related hormone effects
- Influenza Virus Research Studies
- Biotin and Related Studies
- Neonatal Respiratory Health Research
- Respiratory viral infections research
- S100 Proteins and Annexins
- Ferroptosis and cancer prognosis
- Cytomegalovirus and herpesvirus research
- Immune Response and Inflammation
- Vitamin D Research Studies
- Toxoplasma gondii Research Studies
- RNA Interference and Gene Delivery
Jackson Laboratory
2018-2024
University of California, San Francisco
2014
Texas Oncology
2014
Baylor University
2000-2014
Icahn School of Medicine at Mount Sinai
2014
Baylor University Medical Center
2002-2014
Baylor Institute for Rehabilitation
2007-2012
National Institute of Allergy and Infectious Diseases
2007-2008
The University of Texas Southwestern Medical Center
2003
Texas A&M University System
1999
We previously reported (Bell, D., P. Chomarat, D. Broyles, G. Netto, G.M. Harb, S. Lebecque, J. Valladeau, Davoust, K.A. Palucka, and Banchereau. 1999. Exp. Med. 190: 1417–1426) that breast cancer tumors are infiltrated with mature dendritic cells (DCs), which cluster CD4+ T cells. now show infiltrating secrete type 1 (interferon γ) as well high levels of 2 (interleukin [IL] 4 IL-13) cytokines. Immunofluorescence staining tissue sections revealed intense IL-13 on The expression...
The human breast tumor microenvironment can display features of T helper type 2 (Th2) inflammation, and Th2 inflammation promote development. However, the molecular cellular mechanisms contributing to in tumors remain unclear. Here, we show that cancer cells produce thymic stromal lymphopoietin (TSLP). Breast supernatants, a TSLP-dependent manner, induce expression OX40L on dendritic (DCs). OX40L+ DCs are found primary infiltrates. drive development inflammatory producing interleukin-13...
In comparison to murine dendritic cells (DCs), less is known about the function of human DCs in tissues. Here, we analyzed, by using lung tissues from humans and humanized mice, role CD1c+ CD141+ determining type CD8+ T cell immunity generated live-attenuated influenza virus (LAIV) vaccine. We found that both DC subsets acquired antigens vivo expanded specific cytotoxic vitro. However, lung-tissue-resident DCs, but not were able drive CD103 expression on promoted accumulation epithelia vitro...
Abstract Inflammation affects tumor immune surveillance and resistance to therapy. Here, we show that production of IL1β in primary breast cancer tumors is linked with advanced disease originates from tumor-infiltrating CD11c+ myeloid cells. triggered by cell membrane–derived TGFβ. Neutralizing TGFβ or IL1 receptor prevents progression humanized mouse model. Patients metastatic HER2− display a transcriptional signature inflammation the blood leukocytes, which attenuated after blockade. When...
Our studies showed that tumor-infiltrating dendritic cells (DC) in breast cancer drive inflammatory Th2 (iTh2) and protumor inflammation. Here, we show intratumoral delivery of the β-glucan curdlan, a ligand dectin-1, blocks generation iTh2 prevents progression vivo. Curdlan reprograms DCs via ligation enabling to become resistant cancer-derived thymic stromal lymphopoietin (TSLP), produce IL-12p70, favor Th1 cells. activated but not those with TLR-7/8 or poly I:C, induce CD8+ T express...
Abstract Monocyte-derived dendritic cells (MDDCs) activate naive T lymphocytes to induce adaptive immunity, effecting Th1 polarization through IL-12. However, little is known about other potential DC polarizing mechanisms, or how cell may be affected by DCs differentiating in, exposed to, a proinflammatory environment. Macrophages (MΦs) are precursors abundant in inflamed tissues, lymph nodes, and tumors. Thus we studied the cell-activating -polarizing properties of MΦ-derived (ΦDCs)....
The phytopathogenic fungus Botrytis cinerea can infect an extremely wide range of host plants (tomato, grapevine, strawberry, and flax) without apparent specialization. While studying genetic diversity in this fungus, we found element which is present multiple copies dispersed throughout the genome some its isolates. DNA sequence analysis revealed that contained direct, long-terminal repeats (LTRs) 596 bp whose features were characteristic retroviral retrotransposon LTRs. Within element,...
Abstract Dendritic cells (DCs) play the central role in priming of naive T and differentiation unique effector cells. In this study, using lung tissues blood from both humans humanized mice, we analyzed response human CD1c+ CD141+ DC subsets to live-attenuated influenza virus. Specifically, type CD4+ cell immunity elicited by virus–exposed DCs. Both induce proliferation allogeneic with capacity secrete IFN-γ. However, DCs are uniquely able IL-4– IL-13–producing IL-13–secreting through OX40...
Modulation of immune function at the tumor site could improve patient outcomes. Here, we analyze samples metastatic melanoma, a responsive to T cell-based therapies, and find that tumor-infiltrating cells are primarily juxtaposed CD14+ monocytes/macrophages rather than melanoma cells. Using immunofluorescence-guided laser capture microdissection, transcriptomes CD3+ cells, CD14 + monocytes/macrophages, in non-dissociated tissue. Stromal display specific transcriptional signature distinct...
Dendritic cells ignite adaptive immunity by priming naïve T lymphocytes. Human monocyte-derived dendritic (MDDCs) infected with Toxoplasma gondii induce T-lymphocyte gamma interferon production and may thus activate T. gondii-specific immunity. However, we now demonstrate that gondii-infected MDDCs are poor at activating lymphocytes unable to specific cytotoxic On the other hand, acquiring nonviable antigens directly, or indirectly through captured apoptotic necrotic cell bodies, potent...
Pre-clinical use of humanized mice transplanted with CD34
The COVID-19 pandemic continues to be a health crisis with major unmet medical needs. early responses from airway epithelial cells, the first target of virus regulating progression toward severe disease, are not fully understood. Primary human air-liquid interface cultures representing broncho-alveolar epithelia were used study kinetics and dynamics SARS-CoV-2 variants infection. infection measured by nucleoprotein expression, was late event appearing between day 4–6 post for Wuhan-like...
Metastasis of melanoma significantly worsens prognosis; thus, therapeutic interventions that prevent metastasis could improve patient outcomes. Here, we show using humanized mice colonization distant visceral organs with is dependent upon a human CD33+CD11b+CD117+ progenitor cell subset comprising <4% the CD45+ leukocytes. Metastatic tumor-infiltrating CD33+ cells from patients and (h)NSG-SGM3 showed converging transcriptional profiles. Single-cell RNA-seq analysis identified gene...
Humanized mice, defined as mice with human immune systems, have become an emerging model to study hematopoiesis, infectious disease, and cancer. Here, we describe the techniques generate humanized NSGF6 using adult CD34
Yu, C. I., Becker, P. Metang, F. Marches, Y. Wang, H. Toshiyuki, J. Banchereau, M. Merad, and A. K. Palucka. 2014. Human CD141+ dendritic cells induce CD4+ T to produce type 2 cytokines. Immunol. 193: [4335–4343][1]. The sixth author’s name was published incorrectly. The correct