A5006764099- Nitric Oxide and Endothelin Effects
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Neuroendocrine regulation and behavior
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Birth, Development, and Health
- Electron Spin Resonance Studies
- Cardiac electrophysiology and arrhythmias
- Eicosanoids and Hypertension Pharmacology
- Hormonal Regulation and Hypertension
- Photoreceptor and optogenetics research
- Circadian rhythm and melatonin
- Conducting polymers and applications
- Advanced Memory and Neural Computing
- Neuropeptides and Animal Physiology
- Biochemical effects in animals
- Neonatal and fetal brain pathology
- Neural dynamics and brain function
- Synthesis and Reactions of Organic Compounds
- Ion Channels and Receptors
- Dispute Resolution and Class Actions
- Chemical Synthesis and Analysis
- Electrolyte and hormonal disorders
Monash University
2016-2025
Australian Regenerative Medicine Institute
2006-2025
Clayton Foundation
2021
American Arbitration Association
2020
University of South Australia
2020
Florey Institute of Neuroscience and Mental Health
2018
Australian National University
2002
University of Mississippi Medical Center
2001
Jackson Memorial Hospital
2001
Royal Women's Hospital
1999
Gram-negative bacterial peptidoglycan is specifically recognized by the host intracellular sensor NOD1, resulting in generation of innate immune responses. Although epithelial cells are normally refractory to external stimulation with peptidoglycan, these have been shown respond a NOD1-dependent manner pathogens that can either invade or secrete factors into cells. In present work, we report bacteria deliver cytosolic NOD1 via novel mechanism involving outer membrane vesicles (OMVs). We...
Abstract — The nature of the vasodilator endothelium-derived hyperpolarizing factor (EDHF) is controversial, putatively involving diffusible factors and/or electrotonic spread hyperpolarization generated in endothelium via myoendothelial gap junctions (MEGJs). In this study, we investigated relationship between existence MEGJs, endothelial cell (EC) hyperpolarization, and EDHF-attributed smooth muscle (SMC) two different arteries: rat mesenteric artery, where EDHF-mediated vasodilation...
Increasing evidence links vitamin D deficiency and cardiovascular dysfunction in human adults. There is a worldwide increase the prevalence of women reproductive age, particularly dark-skinned and/or veiled their infants. We used rat model to determine functional impact during intra uterine early life on resistance artery reactivity blood pressure offspring as young Rat dams were maintained deficient or replete chow before pregnancy lactation. The same until studied at 7-8 weeks age....
Electroactive materials have been investigated as next-generation neuronal tissue engineering scaffolds to enhance regeneration and functional recovery after brain injury. Graphene, an emerging scaffold material with charge transfer properties, has shown promising results for cell survival differentiation in vitro. In this vivo work, electrospun microfiber coated self-assembled colloidal graphene, were implanted into the striatum or subventricular zone of adult rats. Microglia astrocyte...
Autism Spectrum Disorder (ASD) is a neurodevelopmental condition characterized by social deficits, repetitive behaviours and lack of empathy. Its significant genetic heritability potential comorbidities often lead to diagnostic therapeutic challenges. This review addresses the biological basis ASD, focusing on sex differences in gene expression hormonal influences. ASD more commonly diagnosed males at ratio 4:1, indicating oversight female-specific research risk underdiagnosis females. We...
1 Membrane currents attributed to endothelium-derived hyperpolarizing factor (EDHF) were recorded in short segments of submucosal arterioles guinea-pigs using single microelectrode voltage clamp. The functional responses and human subcutaneous, rat hepatic guinea-pig coronary arteries also assessed as changes membrane potential simultaneously with contractile activity. 2 current-voltage (I-V) relationship for the conductance due EDHF displayed outward rectification little dependence....
1. Membrane potential was recorded with intracellular microelectrodes from the smooth muscle of coronary arteries guinea‐pigs, and responses to endothelium‐derived relaxants were studied under a variety conditions. 2. Stimulation endothelium brief applications acetylcholine or substance P evoked concentration‐dependent hyperpolarizations that complex in nature. A transient component, which is likely result hyperpolarizing factor (EDHF), followed by slow component resulted production nitric...
The recent introduction of technologies capable reprogramming human somatic cells into induced pluripotent stem (iPS) offers a unique opportunity to study many aspects neurodegenerative diseases in vitro that could ultimately lead novel drug development and testing. Here, we report for the first time dermal fibroblasts from patient with relapsing-remitting Multiple Sclerosis (MS) were reprogrammed pluripotency by retroviral transduction using defined factors (OCT4, SOX2, KLF4, c-MYC). MSiPS...
People with Down syndrome (DS) exhibit abnormal brain structure. Alterations affecting neurotransmission and signalling pathways that govern function are also evident. A large number of genes simultaneously expressed at levels in DS; therefore, it is a challenge to determine which gene(s) contribute specific abnormalities, then identify the key molecular involved. We generated RCAN1-TG mice study consequences RCAN1 over-expression investigate contribution phenotype DS. structural...
High-fat diet (HFD) feeding causes ghrelin resistance in arcuate neuropeptide Y (NPY)/Agouti-related peptide neurons. In the current study, we investigated time course over which this occurs and mechanisms responsible for resistance. After 3 weeks of HFD feeding, neither peripheral nor central increased food intake or activated NPY neurons as demonstrated by a lack Fos immunoreactivity whole-cell patch-clamp electrophysiology. Pair-feeding studies that matched calorie with chow show exposure...
Intrauterine growth restriction (IUGR) is associated with impaired cardiac function in childhood and linked to short- long-term morbidities. Placental dysfunction underlies most IUGR, causes fetal oxidative stress which may impact on development. Accordingly, we investigated whether antenatal melatonin treatment, possesses antioxidant properties, afford cardiovascular protection these vulnerable fetuses. IUGR was induced sheep fetuses using single umbilical artery ligation day 105-110 of...
1. Membrane potential and tension were measured simultaneously in ring segments of main coronary artery guinea‐pigs. The synthetic thromboxane A2 analogue U46619 depolarized the tissues from ‐58 +/‐ 2 to ‐40 1 mV increased by 12 mN mm‐1. Nitric oxide (NO) Iloprost, stable prostacyclin, evoked hyperpolarization relaxation. 2. concentration NO required evoke half‐maximal (EC50 x 10(‐5) M) was 40‐fold higher than that which induce relaxation 5 10(‐7) M). EC50 for Iloprost‐induced (3 10(‐8)...
Abstract The deposition of transthyretin (TTR) amyloid in the PNS is a major pathological feature familial amyloidotic polyneuropathy. aim present study was to examine whether TTR could disrupt cytoplasmic Ca 2+ homeostasis and determine role aggregation this process. amyloidogenic examined by solution turbidity, dynamic light scattering atomic force microscopy. A nucleation‐dependent polymerization process observed which formed low molecular weight aggregates (oligomers < 100 nm...
1. The effects of heavy maternal alcohol consumption during pregnancy on cognitive and behavioural performance craniofacial malformations in the offspring have been studied extensively. In contrast, impact intake cardiovascular system more modest received very scant consideration. 2. Adverse conditions pre- neonatal periods can a profound legacy health, including risk disease. Prenatal exposure modulate vascular reactivity, endothelial smooth muscle function. 3. Other prenatal are emerging,...
Abstract The last pregnancy trimester is critical for fetal brain development but a vulnerable period if the compromised by growth restriction (FGR). impact of FGR on maturational neuronal morphology not known, however, studies in sheep allow longitudinal analysis long gestation species. Here we compared hippocampal neuron dendritogenesis and control at three timepoints equivalent to third pregnancy, complemented magnetic resonance image volume, electrophysiology synaptic function. We...
Endothelium-derived hyperpolarizing factor (EDHF)-attributed hyperpolarizations and relaxations were recorded simultaneously from submucosal arterioles of guinea pigs with the use intracellular microelectrodes a video-based system, respectively. Membrane currents electrically short segments under single-electrode voltage clamp. Substance P evoked an outward current current-voltage relationship that was well described by Goldman-Hodgkin-Katz equation for K + current, consistent involvement...
Glycyrrhetinic acid (GA) derivatives have been used to implicate gap junctions in vasorelaxation attributed endothelium-derived hyperpolarizing factor (EDHF). The aim of this study was assess whether GA compounds affect endothelial cell hyperpolarization. Membrane potentials were recorded from dye-identified and smooth muscle cells guinea pig coronary rat mesenteric arteries. had varied effects on the resting membrane potential: depolarization, hyperpolarization, or no effect, depending...
The role of endothelium‐derived hyperpolarizing factor (EDHF) in the regulation blood flow vivo was examined mesenteric and hindlimb circulations anaesthetized rats. Basal conductance decreased from 57 ± 5 to 20 6 μl min −1 mmHg when nitric oxide (NO) production inhibited, combined blockade intermediate‐ small‐conductance Ca 2+ ‐activated K + (K ) channels with charybdotoxin (ChTx) apamin had no further effect. reduced 39 3 22 2 by NO synthesis inhibition, effect channel blockers....
Invasive neural electrodes prepared from materials with a miniaturized geometrical size could improve the longevity of implants by reducing chronic inflammatory response. Graphene-based microfibers tunable porous structures have large electrochemical surface area (ESA)/geometrical (GSA) ratio that has been reported to possess low impedance and high charge injection capacity (CIC), yet control structure remains be fully investigated. In this study, we introduce wet-spun graphene-based pores...
1. The effects of prostaglandins E2 (PGE) and F2alpha (PGF) on membrane potential isometric tension cytoplasmic free calcium concentration ([Ca2+]i) were studied in strips uterine smooth muscle obtained from women undergoing Caesarean delivery at term during established labour. 2. Prostaglandins (PGs) evoked a biphasic response. excitatory component consisted depolarization the membrane, which initiated spike action potentials, an increase [Ca2+]i development. remained depolarized -19 +/- 1...