A5036945050- Adenosine and Purinergic Signaling
- Sleep and Wakefulness Research
- Medical Imaging Techniques and Applications
- Sleep and Work-Related Fatigue
- Sleep and related disorders
- EEG and Brain-Computer Interfaces
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Radiomics and Machine Learning in Medical Imaging
- Advanced MRI Techniques and Applications
- Bipolar Disorder and Treatment
- Functional Brain Connectivity Studies
- Heart Rate Variability and Autonomic Control
- Glioma Diagnosis and Treatment
- Neuroscience of respiration and sleep
- Neurotransmitter Receptor Influence on Behavior
- Advanced Radiotherapy Techniques
- Child Abuse and Trauma
- Electroconvulsive Therapy Studies
- Child Welfare and Adoption
- Radiopharmaceutical Chemistry and Applications
- Pregnancy-related medical research
- Tryptophan and brain disorders
- Attention Deficit Hyperactivity Disorder
- Optical Imaging and Spectroscopy Techniques
University Hospital Cologne
2025
University of Cologne
2025
Forschungszentrum Jülich
2015-2024
Adenosine and functional A1 adenosine receptor (A1AR) availability are supposed to mediate sleep-wake regulation cognitive performance. We hypothesized that cerebral A1AR after an extended wake period decreases a well-rested state recovery sleep. [18F]CPFPX positron emission tomography was used quantify in 15 healthy male adults 52 h of sleep deprivation following 14 Data were additionally compared with values 8 baseline from earlier dataset. Polysomnography, performance, sleepiness...
Serotonin and its cerebral receptors play an important role in sleep-wake regulation. The aim of the current study is to investigate effect 24-h total sleep deprivation on apparent serotonin 2A receptor (5-HT(2A)R) binding capacity human brain test hypothesis that induces global molecular alterations cortical serotonergic system.Volunteers were tested twice with subtype-selective radiotracer [(18)F]altanserin positron emission tomography (PET) for imaging 5-HT(2A)Rs at baseline after 24 h...
Summary The metabotrophic subtype 5 glutamate receptor ( mG luR5) plays a critical role in synaptic plasticity besides its involvement numerous neurological disorders, such as depression. As luR5 availability humans is altered sleep deprivation, we hypothesized that underlies circadian variation. To investigate whether potential changes measured density randomized fashion at six different daytimes 11 adult Sprague–Dawley rats. was quantified by positron emission tomography PET ) using the...
Trait-like differences in cognitive performance after sleep loss put some individuals more at risk than others, the basis of such disparities remaining largely unknown. Similarly, interindividual impairment response to alcohol intake have been observed. We tested whether impairments due either acute or chronic can be predicted by an individual's vulnerability intake. Also, we used positron emission tomography (PET) test infusion results up-regulation cerebral A1 adenosine receptors (A1ARs),...
The spatial extent of amyloid, tau, and neurodegeneration, derived from PET imaging, has higher diagnostic performance for cognitive impairment severity compared with standardized uptake value ratios.
Summary Although chronic sleep restriction frequently produces long‐lasting behavioural and physiological impairments in humans, the underlying neural mechanisms are unknown. Here we used a rat model of to investigate role brain adenosine noradrenaline systems, known regulate wakefulness, respectively. The density A1 A2a receptors β ‐adrenergic before, during following 5 days was assessed with autoradiography. Rats ( n = 48) were sleep‐deprived for 18 h day −1 consecutive SR 1– 5), followed...
Abstract Sleep deprivation (SD) could amplify the temporal fluctuation of spontaneous brain activities that reflect different arousal levels using a dynamic functional connectivity (dFC) approach. Therefore, we intended to evaluate test–retest reliability dFC characteristics during rested wakefulness (RW), and explore how properties these states were affected by extended durations acute sleep loss (28/52 hr). We acquired resting‐state fMRI neuropsychological datasets in two independent...
Abstract Adenosine, its interacting A 1 and 2A receptors, particularly the variant rs5751876 in gene ADORA2A have been shown to modulate anxiety, arousal, sleep. In a pilot positron emission tomography (PET) study healthy male subjects, we suggested an effect of on vivo brain receptor (A AR) availability. As female sex adenosinergic/dopaminergic interaction partners might impact this AR availability, aimed (1) further investigate male-based findings independent, newly recruited cohort...
The neuromodulator adenosine and its receptors are mediators of sleep-wake regulation which is known to differ between sexes. We, therefore, investigated sex differences in A1 receptor (A1AR) availability healthy human subjects under well-rested conditions using [18F]CPFPX positron emission tomography (PET). PET scans were acquired 50 participants (20 females; mean age ± SD 28.0 5.3 years). Mean binding potential (BPND; Logan's reference tissue model with cerebellum as region) volume...
In vivo imaging of the A1 adenosine receptor (A1AR) using (18)F-8-cyclopentyl-3-(3-fluoropropyl)-1-propylxanthine ((18)F-CPFPX) and PET has become an important tool for studying physiologic pathologic states human brain. However, dedicated experimental settings small-animal studies are still lacking. The aim present study was therefore to develop evaluate suitable pharmacokinetic models quantification cerebral A1AR in high-resolution PET.On a animal scanner, 15 rats underwent (18)F-CPFPX...
Scientists have hypothesized that the availability of phosphocreatine (PCr) and its ratio to inorganic phosphate (Pi) in cerebral tissue form a substrate wakefulness. It follows then, according this hypothesis, exhaustion PCr decline Pi fatigue. We used
Previous resting-state fMRI (Rs-fMRI) and positron emission tomography (PET) studies have shown that sleep deprivation (SD) affects both spontaneous brain activity A1 adenosine receptor (A1AR) availability. Nevertheless, the hypothesis neuromodulatory adenosinergic system acts as regulator of individual neuronal remains unexplored.Therefore, fourteen young men underwent Rs-fMRI, A1AR PET scans, neuropsychological tests after 52 h SD 14 recovery sleep.Our findings suggested higher...
The EEG alpha rhythm (∼ 8-13 Hz) is one of the most salient human brain activity rhythms, modulated by level attention and vigilance related to cerebral energy metabolism. Spectral power in range wakefulness sleep strongly varies among individuals based on genetic predisposition. Knowledge about underlying genes scarce, yet small studies indicated that variant rs5751876 gene encoding A2A adenosine receptors (ADORA2A) may contribute inter-individual variation. neuromodulator directly linked...
Introduction Sleep deprivation and electroconvulsive therapy (ECT) effectively ameliorate symptoms in major depressive disorder (MDD). In rodents, both are associated with an enhancement of cerebral adenosine levels, which turn likely influence adenosinergic receptor expression. The aim the current study was to investigate A 1 (A AR) availability patients MDD as a potential mediating factor antidepressant effects ECT using [ 18 F]CPFPX positron emission tomography (PET). Methods Regional AR...
Since animal experiments cannot be completely avoided, the pain, suffering, and distress of laboratory animals must minimized. To this end, a major prerequisite is reliable assessment pain distress. Usually, evaluation welfare done by visual inspection score sheets. However, relatively little known about whether standardized, but subjective, sheets are able to reliably reflect status animals. The current study aimed compare scores changes in body weight with concentrations fecal...
Objective. In positron emission tomography (PET) rigid motion correction, erroneous tracking information translates into reduced quality in corrected reconstructions. We aim to improve the accuracy of data, reconstructions.Approach. developed a method for correction marker/skin displacement over skull, methods which require multiple markers attached on subject head. Additionally, we correct small magnitude (∼1-2 mm) residual translation errors that can still be present after other...
To prevent motion artifacts in small animal positron emission tomography (PET), animals are routinely scanned under anesthesia or physical restraint. Both may potentially alter metabolism and neurochemistry. This study investigates the feasibility of fully awake acquisition subsequent absolute quantification dynamic brain PET data via pharmacokinetic modelling moving rats using glutamate 5 receptor radioligand [
Motivation: We developed a deep learning model to enhance the image quality of ultra-low dose brain PET. Goal(s): Significantly reducing injected not only minimizes radiation risk in subjects but also provides options for scanning protocols, and more follow-up studies. Approach: proposed 3D-Residual Attention U-Net initially trained on whole-body [18F]FDG PET/MR images. used transfer approach fine-tune our [18F]CPFPX PET/MRI inhouse dataset. Results: achieved improved metrics compared with...
In vivo imaging for the A1 adenosine receptors (A1ARs) with positron emission tomography (PET) using 8-cyclopentyl-3-(3-[18F]fluoropropyl)-1-propylxan- thine ([18F]CPFPX) has become an important tool studying physiological processes quantitatively in mice. However, measurement of arterial input functions (AIFs) on mice is a method restricted applicability because small total blood volume and related difficulties withdrawing blood. Therefore, aim this study was to extract appropriate...