A5048439735- Advanced MRI Techniques and Applications
- Ovarian cancer diagnosis and treatment
- Cardiac Imaging and Diagnostics
- Advanced Neuroimaging Techniques and Applications
- MRI in cancer diagnosis
- PI3K/AKT/mTOR signaling in cancer
- Lanthanide and Transition Metal Complexes
- Trace Elements in Health
- Radiomics and Machine Learning in Medical Imaging
- Radiation Dose and Imaging
- Cardiac electrophysiology and arrhythmias
- Effects of Radiation Exposure
- Medical Imaging Techniques and Applications
- Endometriosis Research and Treatment
- Single-cell and spatial transcriptomics
- Carcinogens and Genotoxicity Assessment
- Cancer Mechanisms and Therapy
- Occupational exposure and asthma
- Tissue Engineering and Regenerative Medicine
- Endometrial and Cervical Cancer Treatments
- Epigenetics and DNA Methylation
- Coronary Interventions and Diagnostics
- Cardiovascular Function and Risk Factors
- Anodic Oxide Films and Nanostructures
- Fungal Infections and Studies
University of Pennsylvania
2024-2025
University of Colorado Denver
2024
Hospital of the University of Pennsylvania
2018-2024
University of Colorado Anschutz Medical Campus
2024
Biomedical Advanced Research and Development Authority
2013-2023
The University of Texas Southwestern Medical Center
2009-2016
University of Michigan
2011-2016
Georgia Institute of Technology
2008-2013
Bipar
2013
Augusta University
2007-2012
Endometrioid carcinoma (EC) is a relatively indolent ovarian subtype that nonetheless deadly if detected late. Existing genetically engineered mouse models (GEMMs) of the disease, based on transformation surface epithelium (OSE), take advantage known EC driver gene lesions, but do not fully recapitulate disease features seen in patients. An model which Apc and Pten tumour suppressor genes are conditionally deleted murine OSE yields tumours biologically more aggressive significantly less...
Abstract Recently the dual properties of manganese ion (Mn 2+ ) as an MRI contrast agent and a calcium analogue to enter excitable cells has been used mark specific in brain potential intracellular cardiac agent. Here hypothesis that vivo manganese‐enhanced (MEMRI) can detect changes inotropy mouse heart tested. T 1 ‐weighted images were acquired every minute during experimental time course 75 min. Varying doses Mn (3.3–14.0 nmoles/min/g BW) infused control altered with dobutamine (positive...
Abstract Purpose To compare atherosclerotic plaque uptake of a first (ferumoxtran‐10) and second generation (ferumoxytol) ultrasmall superparamagnetic iron oxide (USPIO) contrast agent with different pharmacokinetic/pharmacodynamic properties. Materials Methods New Zealand White rabbits maintained on high cholesterol/fat diet were subjected to balloon injury the abdominal aorta. Ferumoxtran‐10 or ferumoxytol (500 μmol/kg) was administered at 2, 4, 8 weeks following injury. In vivo magnetic...
Abstract Purpose: Genetically engineered mouse (GEM) models of ovarian cancer that closely recapitulate their human tumor counterparts may be invaluable tools for preclinical testing novel therapeutics. We studied murine endometrioid adenocarcinomas (OEA) arising from conditional dysregulation canonical WNT and PI3K/AKT/mTOR pathway signaling to investigate response conventional chemotherapeutic drugs mTOR or AKT inhibitors. Experimental Design: OEAs were induced by injection adenovirus...
A dualistic pathway model of ovarian carcinoma (OvCA) pathogenesis has been proposed: type I OvCAs are low grade, genetically stable, and relatively more indolent than II OvCAs, most which high-grade serous carcinomas. Endometrioid OvCA (EOC) is a prototypical tumor, often harboring mutations that affect the Wnt phosphatidylinositol 3-kinase/AKT/mammalian target rapamycin signaling pathways. Molecular histopathologic analyses indicate share overlapping features, subset EOCs may undergo...
Abstract Background: Spatial transcriptomics (ST) is revolutionizing our understanding of tumor heterogeneity by providing high-resolution, location-specific gene expression mapping across the microenvironment (TME). Despite its transformative potential, ST’s high cost limits widespread clinical application, particularly for large-scale biomarker discovery. Methods: To address these challenges, we developed Path2Space, a deep learning model trained on ST data, to predict spatial directly...
Abstract Motivation: The rise of spatial transcriptomics (ST) is transforming our understanding tumor heterogeneity by enabling high-resolution, location-specific mapping gene expression across the microenvironment. However, translational potential still limited its high cost, hindering assembly large patient cohorts needed for robust biomarker discovery. Approach: Here we present Path2Space, a deep learning model trained on data to predict directly from histopathology slides. Studying...
Abstract To evaluate first‐pass renal perfusion with ultrasmall superparamagnetic iron oxide (USPIO) particles by MRI, 40 normal rats (20 Dark Agouti (DA) and 20 Brown Norway (BN) rats) 16 transplanted (12 allografts four isografts) were studied on day 4 post‐transplantation different USPIO doses (3.0–18.1 mg Fe/kg/body weight). All animals underwent 128 consecutive snapshot fast low‐angle shot (FLASH) coronal dynamic studies in 43 s. In the rats, a larger maximum signal decrease (MSD)...
At this time, the pathophysiology of macrophage involvement and their role in stroke progression are poorly understood. Recently, T2- T2*-weighted magnetic resonance imaging (MRI), after intravenous administration iron-oxide particles, have been used to understand inflammatory cascade. Earlier studies report that image enhancement is from iron-laden macrophages; however, they do not account for potential blood-brain barrier disruption nonspecific contrast enhancement. In study, spontaneously...
Abstract Micrometer‐sized iron oxide particles (MPIO) are a more sensitive MRI contrast agent for tracking cell migration compared to ultrasmall particles. This study investigated the temporal relationship between inflammation and tissue remodeling due myocardial infarction (MI) using MPIO‐enhanced MRI. C57Bl/6 mice received an intravenous MPIO injection labeling, followed by surgically induced MI seven days later ( n = 7). For controls, two groups underwent either sham‐operated surgery...
Previously, it was shown that selective deletion of peroxisome proliferator activated receptor delta (PPARdelta) in the heart resulted a cardiac lipotoxicity, hypertrophy, and failure. The aim present study to determine effects chronic pharmacological activation PPARdelta model congestive PPARdelta-specific agonist treatment (GW610742X at 30 100 mg/kg/day for 6-9 weeks) initiated immediately postmyocardial infarction (MI) Sprague-Dawley rats. Magnetic resonance imaging/spectroscopy used...
Abstract Manganese has been used as a T 1 ‐weighted MRI contrast agent in variety of applications. Because manganese ions (Mn 2+ ) enter viable myocardial cells via voltage‐gated Ca channels, manganese‐enhanced is sensitive to the viability and inotropic state heart. In spite established importance regulation heart both before after injury, monitoring strategies assess homeostasis affected cardiac tissues are limited. This study implements ‐mapping method obtain quantitative information...
Abstract Owing to its signal‐enhancing characteristics in viable well‐perfused tissue, divalent manganese (Mn 2+ ) has been used as a myocardial imaging contrast agent. Because Mn can enter excitable cells through the voltage‐gated L‐type calcium channels, manganese‐enhanced MRI (MEMRI) determine viability and inotropic state of heart. In this study, we examined correlation between left ventricular infarction zone assessed by cardiac MEMRI function mice with permanent coronary artery...
Abstract The ability of MnCl 2 to enhance canine myocardium and delineate ischemic areas is demonstrated. A dose–response curve was measured using T 1 weighted images in 11 dogs. (36, 113, 360, 3600 μmol) infused over a period 3 min. Signal intensity increased linearly with dose. At 113 μmol (∼10 μmol/kg) the steady‐state increase averaged 212 ± 34%. No significant physiologic effects due were detected except at highest dose where there cardiac depressive effect. Ischemia induced by...
Abstract The sodium–calcium exchanger (NCX) is one of the transporters contributing to control intracellular calcium (Ca 2+ ) concentration by normally mediating net Ca efflux. However, reverse mode NCX can cause overload, which exacerbates myocardial tissue injury resulting from ischemia. Although inhibitor SEA0400 has been shown therapeutically reduce injury, no in vivo technique exists monitor fluctuations produced this drug. Cardiac manganese‐enhanced MRI (MEMRI) may indirectly assess...
Abstract The rise of spatial transcriptomics (ST) is transforming our understanding tumor heterogeneity by enabling high-resolution, location-specific mapping gene expression across the microenvironment. However, translational potential still limited its high cost, hindering assembly large patient cohorts needed for robust biomarker discovery. Here we present Path2Space , a deep learning model trained on data to predict directly from histopathology slides. Studying breast cancer, was first...
Prolonged ischemia causes cellular necrosis and myocardial infarction (MI) via intracellular calcium (Ca(2+)) overload. Manganese-enhanced MRI indirectly assesses Ca(2+) influx movement in vivo as manganese (Mn(2+)) is a analog. To characterize Mn(2+) efflux properties, T(1)-mapping manganese-enhanced studies were performed on adult male C57Bl/6 mice which was altered using pharmacological intervention agents or MI-inducing surgery. Results showed that (1) rate increased exponentially with...