A5044496792- Mesenchymal stem cell research
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Skin Protection and Aging
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Silk-based biomaterials and applications
- Wound Healing and Treatments
- Virus-based gene therapy research
- Estrogen and related hormone effects
- Bone and Joint Diseases
- Hip disorders and treatments
- Metabolism, Diabetes, and Cancer
- Microfluidic and Bio-sensing Technologies
- Viral Infectious Diseases and Gene Expression in Insects
- Periodontal Regeneration and Treatments
- CAR-T cell therapy research
- Cancer-related Molecular Pathways
- Dendrimers and Hyperbranched Polymers
- Endometriosis Research and Treatment
- T-cell and B-cell Immunology
- TGF-β signaling in diseases
- Cancer Genomics and Diagnostics
- Muscle Physiology and Disorders
Cell Biotech (South Korea)
2024
Biotechnology Research Center
2021
Origin Energy (Australia)
2019
Korea University
2003-2011
Seoul National University of Science and Technology
2011
Abstract Background Recently, investigators have been trying to apply the by-products as well stem cells themselves various fields such pharmaceuticals, medical devices, quasi-drug, cosmetis, etc. We aimed comfirm anti-senescence effect of 3D cultured adipose tissue-derived mesenchymal cell–conditioned medium (3D ADMSCs-CM) and develop them cosmetic raw materials for anti-aging purposes. Methods investigated ADMSCs-CM on collagen production performed efficacy tests evaluate a cream-based...
Aims: Dental tissue has been the focus of attention as an easily accessible postnatal source high-quality stem cells. Since first report on dental pulp cells (DPSCs) from permanent third molar teeth, human exfoliated deciduous teeth (SHED) were identified a population distinct DPSCs. In this study, we compared DPSCs supernumerary and SHED in three age- sex-matched patients. Patients & methods: samples obtained patients, who 6 years old male, with parental consent donors, then isolated for...
The nanoscale spatiotemporal resolution of single-particle tracking (SPT) renders it a powerful method for exploring single-molecule dynamics in living cells or tissues, despite the disadvantages using traditional organic fluorescence probes, such as weak fluorescent signal against strong cellular autofluorescence background coupled with fast-photobleaching rate. Quantum dots (QDs), which enable targets multiple colors, have been proposed an alternative to dyes; however, they are not ideally...
Abstract Life-long regeneration of healthy muscle by cell transplantation is an ideal therapy for patients with degenerative diseases. Yet, obtaining stem cells from very limited due to their exhaustion in disease condition. Thus, development a method obtain myogenic required. Here, we showed that the four transcription factors, Six1, Eya1, Esrrb, and Pax3, converts fibroblasts into induced (iMSCs). The iMSCs effective differentiation multinucleated myotubes also higher proliferation...
Insulin resistance is a metabolic state where insulin sensitivity lower than normal condition and strongly related to type 2 diabetes. However, an in vitro model mimicking rare thus screening drugs for severely depends on vivo model. Here, increase anti-diabetic drug selectivity humans, 3D ADMSCs macrophages were co-cultured with in-house fabricated co-culture plates.3D plates designed load RAW264.7 cells containing hydrogels separate wells while allowing cell-cell interaction co-culturing...
In previous work, we established an equine induced pluripotent stem cell line (E-iPSCs) from adipose-derived cells (ASCs) using a lentiviral vector encoding four transcription factors: Oct4, Sox2, Klf4, and c-Myc. the current study, attempted to differentiate these E-iPSCs into mesenchymal (MSCs) by serial passaging MSC-defined media for expansion. Differentiation of MSCs was confirmed analyzing expression levels MSC surface markers CD44 CD29, pluripotency Nanog Oct4. Results indicated that...
High-functional cosmetic products combined with the concept of "treatment" cosmetics are being introduced to market. Cosmetic containing a skin-derived microbiome, three-dimensional (3D) stem cell culture medium, and low-molecular-weight collagen introduced, these leading cosmeceutical We aimed confirm potential 3D medium-containing cream as skin-whitening moisturizing product.To determine enhancing effects adipose tissue-derived mesenchymal cell-conditioned media (3D ADMSC-CM) on whitening...
The complex process of bone regeneration is influenced by factors such as inflammatory responses, tissue interactions, and progenitor cells. Currently, multiple traumas can interfere with fracture healing, causing the prolonging or failure healing. In these cases, grafting most effective treatment. However, there are several drawbacks, morbidity at donor site availability suitable materials. Advantages have been provided in this field a variety stem cell types. Adipose-derived cells (ASCs)...
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This study was performed to evaluate the anticancer effects of tolerable doses metformin with or without medroxyprogesterone (MPA) in endometrial cancer cells. Cell viability, cell invasion, and levels matrix metallopeptidase (MMP) transforming growth factor (TGF)-β1 were analyzed using three human adenocarcinoma lines (Ishikawa, KLE, uterine serous papillary (USPC)) after treatment different dose combinations MPA metformin. Combining (0, 100, 1000 µM) 10 µM induced significantly decreased...
The promyelocytic leukemia gene (PML) encodes a growth/tumor suppressor protein that is essential for the induction of apoptosis in response to various apoptotic signals. mechanism by which PML plays role regulation cell death still unknown. In current study, we demonstrate negatively regulated SAPK2/p38 signaling pathway sequestering p38 from its upstream kinases, MKK3, MKK4, and MKK6, whereas did not affect SAPK1/c-Jun NH(2)-terminal kinase pathway. associated with both vitro vivo carboxyl...
Although mesenchymal stem cells (MSCs) insertion has gained recent attention as a joint-preserving procedure, no study conducted direct intralesional implantation of human umbilical cord-derived MSCs (hUCMSCs) in patients with ONFH. This is protocol for phase 1 clinical trial designed to assess the safety and exploratory efficacy osteoblasts (hUC-Os), osteogenic differentiation-induced from hUCMSCs, early-stage Nine Association Research Circulation Osseous (ARCO) stage or 2 will be assigned...
Although biologiocal ancillay materials (AMs) have specific risk associated with their derivations, it plays key role to manufature cell and gene therapy (CGT) products. It is important understand the regulation relevant AMs for developers.The authors investigated guidelines pharmacopeia (hereinafter referred as "guidelines") biological used manufacture of CGT products in Asia (China, India, Japan, Korea Taiwan). In addition, benchmarked United States (US) European Union (EU).The could be...
Abstract With advances in biotechnology, the field of cryopreservation has been continuously developed and improved. Typical cryo-container was designed with minimal flow to avoid possible structural defects LN2 tank, which a higher thermal conductivity than vapor nitrogen tank. If cells are placed typical stored VN2 cross-contamination can be prevented, but cell viability after thawing may reduced. The structure cryo-containers is not optimized for vaporized quickly into container its...