A5101739607- Mass Spectrometry Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Sulfur Compounds in Biology
- Epigenetics and DNA Methylation
- Advanced Proteomics Techniques and Applications
- Metabolism and Genetic Disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Folate and B Vitamins Research
- Glycosylation and Glycoproteins Research
- Retinoids in leukemia and cellular processes
- Cardiac Ischemia and Reperfusion
- Advanced biosensing and bioanalysis techniques
- Neonatal and fetal brain pathology
- Monoclonal and Polyclonal Antibodies Research
- High Altitude and Hypoxia
- Neuroscience and Neuropharmacology Research
- Vitamin D Research Studies
- Polyamine Metabolism and Applications
- Eicosanoids and Hypertension Pharmacology
- Identification and Quantification in Food
- Histone Deacetylase Inhibitors Research
- Nitrogen and Sulfur Effects on Brassica
- Bone health and osteoporosis research
- Heart Failure Treatment and Management
Shimadzu (Japan)
2005-2023
Keio University
2012-2017
Kyoto Prefectural University of Medicine
2010
Mitsubishi Electric (Japan)
2009
Research Center for Eco-Environmental Sciences
2009
Mukogawa Women's University
2009
Peking University
2009
Osaka University
2001
Tokai University
1999
UCLA Medical Center
1986
Enhancement of cerebral blood flow by hypoxia is critical for brain function, but signaling systems underlying its regulation have been unclear. We report a pathway mediating hypoxia-induced vasodilation in studies monitoring vascular disposition cerebellar slices and intact mouse brains using two-photon intravital laser scanning microscopy. In this cascade, elicits via the coordinate actions H 2 S formed cystathionine β-synthase (CBS) CO generated heme oxygenase (HO)-2. Hypoxia diminishes...
Haem oxygenase (HO)-1/carbon monoxide (CO) protects cancer cells from oxidative stress, but the gas-responsive signalling mechanisms remain unknown. Here we show using metabolomics that CO-sensitive methylation of PFKFB3, an enzyme producing fructose 2,6-bisphosphate (F-2,6-BP), serves as a switch to activate phosphofructokinase-1, rate-limiting glycolytic enzyme. In human leukaemia U937 cells, PFKFB3 is asymmetrically di-methylated at R131 and R134 through modification by protein arginine...
Local responses of energy metabolism during brain ischemia are too heterogeneous to decipher redox distribution between anoxic core and adjacent salvageable regions such as penumbra. Imaging mass spectrometry combined by capillary electrophoresis/mass providing quantitative metabolomics revealed spatio-temporal changes in adenylates NADH a mouse middle-cerebral artery occlusion model. Unlike the where ATP decreased, penumbra displayed paradoxical elevation despite constrained blood supply....
Despite an increasing interest in vitamin D status, a reference range of the nutrient has not been fully established. This is partly due to paucity standardized measuring systems with high throughput. In addition, may vary by populations and change modernization lifestyles.This study aims calculate current concentration 25-hydroxyvitamin (25(OH)D) among healthy people living urban area Japan.A newly developed automated liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) system...
Loss of prolyl hydroxylase 2 (PHD2) activates the hypoxia-inducible factor-dependent hypoxic response, including anaerobic glycolysis, which causes large amounts lactate to be released from cells into circulation. We found that Phd2-null mouse embryonic fibroblasts (MEFs) produced more than wild-type MEFs, as expected, whereas systemic inactivation PHD2 in mice did not cause hyperlacticacidemia. This unexpected observation led us hypothesize response activated liver enhances Cori cycle, a...
Abstract Histone acetylation has been linked to cardiac hypertrophy and heart failure. However, the pathological implications of changes in histone methylation effects interventions with methyltransferase inhibitors for failure have not fully clarified. Here, we focused on H3K9me3 status investigated H3K9 inhibitor chaetocin prognoses Dahl salt-sensitive rats, an animal model chronic Chaetocin prolonged survival restored mitochondrial dysfunction. ChIP-seq analysis demonstrated that stress...
Retinoic acid receptor (RAR) agonists are speculated to be one possible cause for the widely observed frog deformities in North America, although little is known about specific RAR aquatic environments. We identified sewage treatment plants (STPs) and receiving rivers using an yeast two-hybrid bioassay. Water samples were extracted by solid-phase extract cartridges, which successively eluted hexane, ethyl acetate, methanol Among three fractions, acetate fraction showed highest agonistic...
Although therapeutic hypothermia is known to improve neurologic outcomes after perinatal cerebral hypoxia-ischemia, etiology remains unknown. To decipher the mechanisms whereby regulates metabolic dynamics in different brain regions, we used a two-step approach: metabolomics target pathways responding cooling, and quantitative imaging mass spectrometry reveal spatial alterations targeted metabolites brain. Seven-day postnatal rats underwent permanent ligation of left common carotid artery...
We constructed a system for the microscale identification of membrane-blotted proteins by proteolytic digestion using an instrument developed with piezoelectric chemical inkjet technology and MS/MS analyses resulting peptides matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight tandem mass spectrometer (MALDI-QIT-TOF MS). Using this system, bovine serum albumin was clearly identified at levels less than 100 fmol, from Escherichia coli extract were also search.
Bile acids have recently emerged as versatile signaling molecules, and their pathway is a promising target for the treatment of metabolic diseases. Here, we developed highly sensitive high-throughput quantification method six taurine- glycine-conjugated bile using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry after solid-phase extraction (SPE-MALDI-TOF MS). In carbon tetrachloride (CCl4)-induced liver injury/regeneration model in mice, serum acid profiles were...
It is difficult to isolate peptide ligands that bind saccharides from phage display r｛mdom library since an interaction between pephdes and likely be restricted hydrogen bond．We perβormed a screening against neutral polysaccharide， chitin in the media consisting of orgImic solvent re(luce non-specmc hydrophobic interachon Imd emphasize bond．As result，the converged
Although therapeutic hypothermia has been shown to improve neurologic outcomes after the perinatal cerebral hypoxia‐ischemia, etiology remains unknown. To decipher mechanisms whereby regulates metabolism in different regions of brain, we took a two‐step approach; metabolomics target metabolic pathways responding lowering temperature, and quantitative imaging mass spectrometry (Q‐IMS) reveal spatial alterations targeted metabolites at specific brain. 7‐day postnatal rats underwent permanent...
Although it has been known that brain generates carbon monoxide (CO) via heme oxygenase (HO) catalyzed reactions, physiologic roles of CO in the central nervous system remain elusive. Previous study showed HO-2 an O2-dependent manner. By acting as acute O2 sensor within neurovascular unit, contributes to maintenance cerebral ATP levels against global hypoxia (Morikawa et al., PNAS, 109, 1293-1298). In this study, we examined if deletion exacerbates metabolism upon focal ischemia. We compared...