The latent membrane protein (LMP1) of Epstein-Barr virus (EBV) is expressed in EBV-associated nasopharyngeal carcinoma, which notoriously metastatic. Although it established that LMP1 represses E-cadherin expression and enhances the invasive ability carcinoma cells, mechanism underlying this repression remains to be elucidated. In study, we demonstrate induces activity DNA methyltransferases 1, 3a, 3b, using real-time reverse transcription-PCR enzyme assay. This results hypermethylation...

Abstract Objective Angiogenesis plays a part in the pathogenesis of rheumatoid arthritis (RA), and nanogold inhibits activity an angiogenic factor, vascular endothelial growth factor (VEGF). We therefore investigated whether intraarticular delivery ameliorates collagen‐induced (CIA) rats. Methods Binding 13‐nm to VEGF human RA synovial fluid (SF) its effects on SF–induced cell proliferation migration were assessed. Nanogold was administered intraarticularly rats with CIA before onset...

The miRNA participates in a variety of biologic processes, and dysregulation is associated with malignant transformation. In this study, we determined specific profile oral cancer by using array screening method. There were 23 miRNAs found considerably differential expressions between six cell lines five normal keratinocytes, which, 10 showed the highest significant difference after independent examination reverse transcription quantitative PCR. Eight molecules upregulated, miR-10b,...

Norovirus and rotavirus cause outbreaks of diarrheal disease worldwide. This prospective observational study was undertaken to investigate the clinical characteristics complications, with a focus on convulsive disorders, gastroenteritis caused by norovirus in hospitalized pediatric patients northern Taiwan.Children acute Chang Gung Children's Hospital from August 2004 through January 2007 were enrolled study. Rotavirus detected reverse-transcriptase polymerase chain reaction fecal specimens...

// Hung-Chih Hsu 1, 2 , Tan Kien Thiam 3 Yen-Jung Lu Chien Yuh Yeh 2, 4 Wen-Sy Tsai Jeng Fu You Hsin Yuan Hung Chi-Neu 5 An Hua-Chien Chen Shu-Jen Tsai-Sheng Yang 1 Division of Hematology-Oncology, Chang Gung Memorial Hospital, Kwei-Shan, Tao-Yuan 333, Taiwan College Medicine, University, ACT Genomics, Neihu Dist., Taipei 114, Colon and Rectal Surgery, Graduate Institute Clinical Correspondence to: Chen, e-mail: sjchen@actgenomics.com Yang, a481124@cgmh.org.tw Keywords: BRAF, metastatic...

Backgrounds Glucagon-like peptide-1 receptor agonist (GLP-1 RA) is probably one of more effective antidiabetic agents in treatment type 2 diabetes mellitus (T2D). However, the heterogenicity responses to GLP-1 RA may be potentially related gut microbiota, although no human evidence has been published. This pilot study aims identify microbial signatures associated with glycemic RA. Materials and Methods Microbial compositions 52 patients T2D receiving were determined by 16S rRNA amplicon...

Abstract The aim of the present study was to evaluate microbiota children with severe or complicated acute viral gastroenteritis (AGE). To that end, next-generation sequencing (NGS) technology used sequence 16S ribosomal RNA ( rRNA ) gene in 20 hospitalized pediatric patients AGE and a further otherwise healthy children; fecal microbiome then assessed. Comparative metagenomics data were analyzed by Wilcoxon rank–sum test hierarchical clustering analysis bacterial reads. statistical analyses...

By reverse transcription-PCR or PCR, among 257 children with nonbacterial acute gastroenteritis (AGE), rotavirus, norovirus, astrovirus, enteric adenovirus, and multiple viruses were identified in 78 (30.4%), 21 (8.2%), 7 (2.7%), 51 (19.8%), 53 (20.6%) patients, respectively. Higher disease severity was found for AGE caused by rotavirus alone. The majority of rotaviruses isolated from 2004 to 2006 belonged genotypes G1 (20.4%), G2 (16.5%), G3 (27.2%), G9 (21.4%).

Abstract CD4 + CD25 T cells have been shown to play a regulatory or suppressive role in the immune response and are possibly relevant pathogenesis of autoimmune diseases. In present study, we attempted investigate levels peripheral blood (PB) synovial fluid (SF) patients with rheumatoid arthritis (RA) effects on vitro cytokine production by stimulated SF mononuclear (SFMC). The results showed that RA had similar frequencies PB, expressed as percentages lymphocyte population, did healthy...

Background. Glioblastoma multiforme (GBM) is the most aggressive type of primary brain tumor. It a rapidly progressive, highly recurrent, fatal intracranial neoplasm, and demand for novel treatment urgent. Valproic acid (VPA) potential anticancer agent that belongs to class histone deacetylase (HDAC) inhibitors, targeting epigenetic control gene functions in cancer cells. This drug has been administered prevention or seizure disorder GBM patients; therefore, retrospective analysis may...

The direct sequencing of PCR products generates heterozygous base-calling fluorescence chromatograms that are useful for identifying single-nucleotide polymorphisms (SNPs), insertion-deletions (indels), short tandem repeats (STRs), and paralogous genes. Indels STRs can be easily detected using the currently available In d elligent or ShiftDetector programs, which do not search reference sequences. However, detection other genomic variants remains a challenge due to lack appropriate tools...

Mutations in the human telomerase reverse transcriptase (TERT) promoter contribute to increased TERT activity. The purpose of present study was assess prevalence mutations oral cavity squamous cell carcinoma (SCC).Total DNA extracted from 201 SCC tumors and adjacent normal tissues. Primers were used amplify sequence region containing 2 (C228T C250T) that then sequenced using Sanger method.Sequencing revealed 52.5% (104/201) 12.9% (26/201) tumor tissues 6.0% (12/201) 2.5% (5/201) contained...

Aim . The relationship between genetic polymorphisms of the glucagon-like peptide-1 (GLP-1) receptor ( GLP1R ) gene and unresponsiveness to GLP-1 analogue treatment in patients with poorly controlled type 2 diabetes mellitus (DM) is unclear. Methods Thirty-six DM were enrolled they received six days continuous subcutaneous insulin infusion for this study. After normalization blood glucose first 3 days, then a combination therapy injections analogue, exenatide, another days. All 13 exons...

Abstract Objectives IFI 27 is highly expressed in psoriatic lesions but its function has not been known. The present study aimed to explore role proliferation of epidermal keratinocytes. Materials and methods knockdown over‐expression keratinocytes were used compare their proliferation, by MTT assay, apoptosis (by annexin V binding) cell cycle progression flow cytometry. Formation cyclin A/ CDK 1 complex was examined a co‐immunoprecipitaion method. Anti‐proliferation effects also vivo...

The posttransplantation recurrence rate of hepatitis B virus (HBV) infection in patients with chronic (CHB) is underestimated and linked unfavorable outcomes. We investigated HBV by serum assays CHB following liver transplantation. enrolled who underwent transplantation between March 2001 July 2021 to participate cross-sectional testing for HBV-related markers, including biochemical analysis HBsAg core-related antigen (HBcrAg) real-time RT-PCR/PCR RNA DNA, 2022. this study was defined as...

Although human embryonic stem cells (ESCs) and mesenchymal (MSCs) from various sources display immunomodulatory effects, direct comparisons among these classes of have not been reported.Peripheral blood mononuclear cell suppression assays carboxyfluorescein diacetate succinimidyl ester were used to assess the immunosuppressive effects cells. Gene expression was measured using DNA microarrays. Enzyme-linked immunosorbent determine interleukin (IL)-6 levels.We found that fetal-type MSCs...