A5015347918- Genomics and Phylogenetic Studies
- Microbial Natural Products and Biosynthesis
- Microbial Community Ecology and Physiology
- Fire effects on ecosystems
- Analytical Chemistry and Chromatography
- Force Microscopy Techniques and Applications
- Actinomycetales infections and treatment
- Plant Pathogens and Fungal Diseases
- Protist diversity and phylogeny
- Microbial Fuel Cells and Bioremediation
- Viral Infections and Vectors
- Analytical chemistry methods development
- Soil Carbon and Nitrogen Dynamics
- Estrogen and related hormone effects
- Legume Nitrogen Fixing Symbiosis
- Wastewater Treatment and Nitrogen Removal
- Biochemical and Structural Characterization
- Advanced Proteomics Techniques and Applications
- Geochemistry and Geologic Mapping
- Marine Sponges and Natural Products
- Slime Mold and Myxomycetes Research
- Medical Education and Admissions
- Historical Medical Research and Treatments
- Antimicrobial Peptides and Activities
- Algal biology and biofuel production
Swansea University
2010-2024
John Innes Centre
2005-2010
University of Groningen
1994-2005
Norwich Research Park
2005
Clemson University
1998
University College Dublin
1998
Streptomycetes are exploited for production of a wide range secondary metabolites, and there is much interest in enhancing the level these metabolites. Secondary metabolites synthesized dedicated biosynthetic routes, but precursors co-factors derived from primary metabolism. High antibiotics streptomycetes therefore requires engineering Here we demonstrate this by targeting key enzyme glycolysis, phosphofructokinase, leading to improved antibiotic Streptomyces coelicolor A3(2). Deletion...
OPINION article Front. Microbiol., 10 February 2016Sec. Terrestrial Microbiology Volume 7 - 2016 | https://doi.org/10.3389/fmicb.2016.00073
The filamentous bacteria Streptomyces coelicolor and lividans exhibit a complex life cycle. After branched submerged mycelium has been established, aerial hyphae are formed that may septate to form chains of spores. structures possess several surface layers unknown nature make them hydrophobic, one which is the rodlet layer. We have identified two homologous proteins, RdlA RdlB, involved in formation layer both streptomycetes. rdl genes expressed growing but not Immunolocalization showed...
Streptomyces coelicolor A3(2) synthesizes three membrane-associated respiratory nitrate reductases (Nars). During aerobic growth in liquid medium the bacterium was able to reduce 50 mM stoichiometrically nitrite. Construction and analysis of a mutant which all narGHJI operons were deleted showed that it failed nitrate. Deletion gene encoding MoaA, catalyses first step molybdenum cofactor biosynthesis, also prevented reduction, consistent with Nars being molybdoenzymes. In contrast...
Summary The actinomycete Streptomyces coelicolor is an obligate aerobe that found in soil and aqueous habitats. levels of oxygen these environments can vary considerably, which raises the question how bacteria survive during periods anaerobiosis. Although S. cannot grow complete absence oxygen, we demonstrate here it capable microaerobic growth maintaining viability through several weeks strict Both resting germinated spores are able to abrupt exposure anaerobiosis, contrasts situation with...
ABSTRACT Autotrophic growth of Xanthobacter flavus is dependent on the fixation carbon dioxide via Calvin cycle and oxidation simple organic inorganic compounds to provide cell with energy. Maximal induction cbb gap-pgk operons encoding enzymes occurs in absence multicarbon substrates presence methanol, formate, hydrogen, or thiosulfate. The LysR-type transcriptional regulator CbbR regulates expression operons, but it unknown what cellular signal responds. In order study effects...
Phylogenetic reconstruction revealed that most Actinobacterial orthologs of S. coelicolor SCO2837, encoding a metal-dependent galactose oxidase-like protein, are found within Streptomyces and were probably acquired by horizontal gene transfer from fungi. Disruption SCO2837 (glxA) caused conditional bld phenotype could not be reversed extracellular complementation. Studies aimed at characterising the regulation expression glxA showed it is target for other genes. We provide evidence required...
We report the 4,385,577-bp high-quality draft assembly of bacterial symbiont Rhodococcus rhodnii strain LMG5362, isolated from gut Rhodnius prolixus (Hemiptera, Reduviidae, Triatominae), principle vector protozoan Trypanosoma cruzi, etiological agent Chagas disease. This sequence might provide useful information for subsequent studies symbiotic relationship between Rd. and Rc. rhodnii, while also providing a starting point development biotechnological applications control prolixus.
ABSTRACT The LysR-type transcriptional regulator CbbR controls the expression of cbb and gap-pgk operons in Xanthobacter flavus , which encode majority enzymes Calvin cycle required for autotrophic CO 2 fixation. operon promoter this chemoautotrophic bacterium contains three potential binding sites, two partially overlap. Site-directed mutagenesis subsequent analysis DNA by activity were used to show that sequences are functional. Inverted repeat IR 1 is a high-affinity site. main function...
Streptomyces coelicolor differentiates on solid agar media by forming aerial hyphae that septate into spores. We here show differentiation also occurs in standing liquid minimal media. After a period of submerged growth, migrate to the air interface, where they become fixed rigid reflecting film. Colonies result from these form sporulating hyphae. In addition, medium may attach surface. Liquid cultures easily anoxic only 1 2 mm below Yet, biomass increases, implying existence metabolic...
Environmental pollution has strong links to adverse human health outcomes with risks of through production, use, ineffective wastewater (WW) remediation, and/or leachate from landfill. 'Fit-for-purpose' monitoring approaches are critical for better control and mitigation harm, current sample preparation methods complex environmental matrices typically time-consuming labour intensive, unsuitable high-throughput screening. This study shown that a modified 'Quick Easy Cheap Effective Rugged...
Streptomyces coelicolor is an obligate aerobic, filamentous soil-dwelling bacterium. Remarkably, the genome of S. has three copies narGHJI operon that encodes respiratory nitrate reductase. This review summarizes our current views on requirements for multiple reductases in coelicolor.
Multiple-choice question (MCQ) examinations are increasingly used as the assessment method of theoretical knowledge in large class-size modules many life science degrees. MCQ-tests can be to objectively measure factual knowledge, ability and high-level learning outcomes, but may also introduce gender bias performance dependent on topic, instruction, scoring difficulty. The 'Single Answer' (SA) test is often which students choose one correct answer, they unable demonstrate partial knowledge....
Variations at the nanoscale in soil and organic matter distribution are critical to understanding factors involved composition turnover. Atomic Force Microscopy describes physical topographical properties nanoscale, thus represents an important tool nanoscience.
Binding-protein-dependent secondary transporters make up a unique transport protein family. They use solute-binding in proton-motive-force-driven transport. Only few systems have been functionally analysed. The yiaMNO genes of Escherichia coli K-12 encode one family member that transports the rare pentose l -xylulose. Its physiological role is unknown, since wild-type E. does not utilize -xylulose as sole carbon source. Deletion strain MC4100 resulted remarkable changes transition from...
The expression of the cbb and gap–pgk operons Xanthobacter flavus encoding enzymes Calvin cycle is regulated by transcriptional regulator CbbR. In order to identify other genes involved in regulation these operons, a mutant was isolated with lowered activity fusion between promoter operon reporter gene lacZ. This unable grow autotrophically had reduced growth rate medium supplemented gluconate or succinate. indistinguishable from wild-type strain, but induction upon transition autotrophic...
ABSTRACT The levels of reduced and oxidized nicotinamide adenine dinucleotides were determined in Xanthobacter flavus during a transition from heterotrophic to autotrophic growth. Excess reducing equivalents are rapidly dissipated following induction the Calvin cycle, indicating that cycle serves as sink for excess equivalents. physiological data support conclusion previously derived molecular studies expression genes is controlled by intracellular concentration NADPH.
Abstract Accurate measurement of the composition complex samples is key for safety and efficacy a range products used in daily life, with sample preparation critical step this workflow. QuEChERS one such method, however published protocols do not explicitly address acidic, basic, neutral, amphiphilic species single protocol often use extra steps or an alternative to recover breadth chemical types. Our work addresses need by investigating monitoring wide chemistries within environmental...
Although the Park Grass Experiment is an important international reference soil for temperate grasslands, it still lacks direct extraction of its metaproteome. The identification these proteins can be crucial to our understanding ecology and major biogeochemical processes. However, protein from a technically fraught process due difficulties with co-extraction humic material lack compatible databases identify proteins. To address issues, we combined two techniques on experiment soil, one...