A5081448915- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Epilepsy research and treatment
- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Neural dynamics and brain function
- Memory and Neural Mechanisms
- Adenosine and Purinergic Signaling
- Neural Networks and Reservoir Computing
- EEG and Brain-Computer Interfaces
- Retinal Development and Disorders
- Nerve injury and regeneration
- Nicotinic Acetylcholine Receptors Study
- Mitochondrial Function and Pathology
- Eicosanoids and Hypertension Pharmacology
- Advanced Memory and Neural Computing
- Functional Brain Connectivity Studies
- Biochemical effects in animals
- Receptor Mechanisms and Signaling
- Anesthesia and Neurotoxicity Research
Ghent University
2018-2025
Ghent University Hospital
2021-2024
The intrahippocampal kainic acid (IHKA) mouse model is an extensively used in vivo to investigate the pathophysiology of mesial temporal lobe epilepsy (mTLE) and develop novel therapies for drug-resistant epilepsy. It characterized by profound hippocampal sclerosis spontaneously occurring seizures with a major role injected damaged hippocampus, but little known about excitability specific subregions. purpose this study was electrophysiologically characterize subregions chronic phase induced...
Up to 30 % of epilepsy patients suffer from drug-resistant (DRE). The search for innovative therapies is therefore important close the existing treatment gap in these patients. adenosinergic system possesses potent anticonvulsive effects, mainly through adenosine A1 receptor (A1R). However, clinical application A1R agonists hindered by severe systemic side effects. To achieve local modulation A1Rs, we employed a photopharmacological approach using caged version agonist...
Epilepsy is a neurological disorder characterized by recurrent epileptic seizures. Electrophysiological and neuroimaging studies in patients with epilepsy suggest that abnormal functional brain networks play role the development of epilepsy, i.e. epileptogenesis, resulting generation spontaneous seizures cognitive impairment. In this longitudinal study, we investigated changes during epileptogenesis intraperitoneal kainic acid (IPKA) rat model temporal lobe (TLE) using resting state magnetic...
Selective neuromodulation using designer receptors exclusively activated by drugs (DREADDs) has become an increasingly important research tool, as well emerging therapeutic approach. However, the safety profile of DREADD expression is unknown. Here, different titers adeno-associated viral (AAV) vector were administered in attempt to vary total levels inhibitory hM4D(Gi) excitatory hippocampal neurons. Male Sprague Dawley rats injected with AAV2/7 encoding DREADD-mCherry, DREADD, or mCherry....
Abstract Adenosine is a potent regulator of neurotransmission and neuronal excitability through activation G i protein-coupled adenosine A 1 receptors (A Rs). It has gained interest as potential anticonvulsant due to its endogenous involvement in ending ongoing seizure activity. recently developed coumarin-caged derivative the R agonist N 6 -cyclopentyl-adenosine (CPA), cCPA, was used for photo-uncaging CPA with millisecond flashes 405 nm light. At population level, reduces Schaffer...
We report the design, synthesis, and validation of novel compound photocaged N6-cyclopentyladenosine (cCPA) to achieve precisely localized timed release parent adenosine A1 receptor agonist CPA using 405 nm light. Gi protein-coupled receptors (A1Rs) modulate neurotransmission via pre- post-synaptic routes. The dynamics CPA-mediated effect on neurotransmission, characterized by fast activation slow recovery, make it possible implement a closed-loop control paradigm. strength is monitored as...
Abstract Up to 30% of epilepsy patients suffer from drug-resistant (DRE). The search for innovative therapies is therefore important close the existing treatment gap in these patients. adenosinergic system possesses potent anticonvulsive effects, mainly through adenosine A 1 receptor (A R). However, clinical application R agonists hindered by severe systemic side effects. To achieve local modulation Rs, we employed a photopharmacological approach using caged version agonist N 6...
Event Abstract Back to Effect of chemogenetic modulation hippocampal neurons on depression and spatial memory impairment in a rat model for temporal lobe epilepsy. Erine Craey1*, Marie-Gabrielle Goossens1, Jana Desloovere1, Caroline Merckx1, Chris Van Den Haute2, Veerle Baekelandt2, Dimitri De Bundel3, Ilse J. Smolders3, Kristl Vonck1, Paul Boon1 Robrecht Raedt1 1 Department Head Skin, Ghent University, Belgium 2 Neurosciences, KU Leuven, 3 Vrije University Brussel, RATIONALE Major are the...
Event Abstract Back to In vivo effect of activating optogenetic chloride channel on hippocampal evoked potentials Anirudh Acharya1*, Latoya Stevens1, Charlotte Germonpré1, Marie-Gabrielle Goossens1, Wouter Van Lysebettens1, Erine Craey1, Wytse Wadman1, Veerle Baekelandt2, Chris Den Haute2*, Paul Boon1, Alfred Meurs1 and Robrecht Raedt1 1 4Brain, Ghent University Hospital, Belgium 2 Research Group for Neurobiology Gene Therapy, KU Leuven, Background aims: inhibition can be achieved by the...
Event Abstract Back to Evoked hippocampal seizures are associated with bursts of locus coeruleus neuronal activity in the anaesthetized rat: a mechanism ‘pathological memory formation’ epilepsy? Lars E. Larsen1, 2*, Latoya Stevens2, Anirudh Acharya2, Charlotte Bouckaert2, Erine Craey2, Jana Desloovere2, Marie-Gabrielle Goossens2, Germonpré2, Silke Proesmans2, Paul Boon2, Kristl Vonck2, Chris Vanhove1, Pieter Van Mierlo1 and Robrecht Raedt2 1 Medical Imaging Signal Processing, Ghent...