A5101737679- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Clostridium difficile and Clostridium perfringens research
- Bacteriophages and microbial interactions
- Bone fractures and treatments
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Orthopedic Surgery and Rehabilitation
- Helicobacter pylori-related gastroenterology studies
- Biopolymer Synthesis and Applications
- Reproductive Biology and Fertility
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Sperm and Testicular Function
- Gut microbiota and health
- Reproductive System and Pregnancy
- Transport Systems and Technology
- Spine and Intervertebral Disc Pathology
- Hypothalamic control of reproductive hormones
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Flow Measurement and Analysis
- Advancements in Transdermal Drug Delivery
- Regulation of Appetite and Obesity
- Connective tissue disorders research
University of Cincinnati Medical Center
2015-2024
Cadila Healthcare (India)
2021-2023
Babasaheb Bhimrao Ambedkar University
2019-2023
International Centre for Genetic Engineering and Biotechnology
2010-2014
University of Allahabad
2014
Jawaharlal Nehru University
2013
Sarojini Naidu Medical College
1982-2009
University of Delhi
2009
Institute of Genomics and Integrative Biology
2004-2005
Central Drug Research Institute
1967-1974
ZyCoV-D is a DNA vaccine candidate, which comprises plasmid carrying spike-S gene of SARS-CoV-2 virus along with coding for signal peptide. The spike(S) region includes the receptor-binding domain (RBD), binds to human angiotensin converting Enzyme (ACE)-2 receptor and mediates entry inside cell.We conducted single-center, open-label, non-randomized, Phase 1 trial in India between July 2020 October 2020. Healthy adults aged 18 55 years were sequentially enrolled allocated one four treatment...
Pathobionts are opportunistic microbes that emerge as a result of perturbations in the healthy microbiome due to complex interactions various genetic, exposomal, microbial, and host factors lead their selection expansion. Their proliferations can aggravate inflammatory manifestations, trigger autoimmune diseases, severe life-threatening conditions. Current surge research is unwinding these interplays between disease development protection against pathobionts. This review summarizes current...
The apprehension of needles related to injection site pain, risk transmitting bloodborne pathogens, and effective mass immunization have led the development a needle-free system (NFIS). Here, we evaluated efficacy NFIS needle (NIS) for delivery immunogenicity DNA vaccine candidate ZyCoV-D in rhesus macaques against SARS-CoV-2 infection. Briefly, 20 were divided into 5 groups (4 animals each), that is, I (1 mg dose by NIS), II (2 III NFIS), IV NFIS) V (phosphate-buffer saline [PBS])....
ABSTRACT Protein kinases have a diverse array of functions in bacterial physiology, with distinct role the regulation development, stress responses, and pathogenicity. pknF , one 11 Mycobacterium tuberculosis encodes an autophosphorylating, transmembrane serine/threonine protein kinase, which is absent fast-growing, nonpathogenic smegmatis . Herein, we investigate physiological PknF using antisense strategy M. expressing its kinase mutant (K41M) Expression led to reduction growth rate...
Pathogenic strains of mycobacteria produce copious amounts glutamine synthetase (GS) in the culture medium. The enzyme activity is linked to synthesis poly-α-l-glutamine (PLG) cell walls. This study describes a glnA-1 mutant Mycobacterium bovis that produces reduced levels GS. was able grow enriched 7H9 medium without supplementation. strain contained no detectable PLG walls and showed marked sensitivity different chemical physical stresses such as lysozyme, SDS sonication. two...
ABSTRACT The virulence of Mycobacterium tuberculosis is intimately related to its distinctive cell wall. biological significance poly-α- l -glutamine (PLG), a component in the wall virulent mycobacteria, has not been explored adequately. focus this study investigate role locus, Rv0574c, coding for polyglutamate synthase-like protein, synthesis context mycobacterial virulence. Evaluation Rv0574c gene expression M. demonstrated growth-phase-linked induction with concomitant accumulation was...
Abstract Vaccines remain the key protective measure to achieve herd immunity control disease burden and stop COVID-19 pandemic. We have developed assessed immunogenicity efficacy of two formulations (1mg 2mg) ZyCoV-D (a plasmid DNA based vaccine candidates) administered through Needle Free Injection System (NFIS) syringe-needle (intradermal) in rhesus macaques with three dose regimens. The candidate 2mg using elicited a significant immune response development SARS-CoV-2 S1 spike region...
Serine/threonine protein kinases (STPKs) represent a burgeoning concept in prokaryotic signaling and have been implicated range of control mechanisms. This paper describes the enzymatic molecular characterization PknH, mycobacterial STPK. After cloning expression as Glutathione-S-transferase fusion E. coli, PknH was found to phosphorylate itself exogenous substrates like myelin basic histone. The kinase activity inhibited by inhibitors staurosporine H-7. results confirmed that is...
99m Tc‐Diethylene triamine pentaacetic acid‐bis (amide) conjugates have been synthesized and evaluated as a potential radiopharmaceutical for tumor imaging. The compounds were by the condensation reaction of DTPA bis(anhydride) with different l ‐amino acids (methyl tryptophan, 5‐hydroxy tryptophan) characterized on basis IR, NMR, Mass spectroscopy. Tc‐labeled found stable about 24 h under physiological conditions more than 95% radiolabeling yield. Blood kinetic studies all these complexes...
Abstract Background The cell wall of pathogenic mycobacteria is known to possess poly-L-glutamine (PLG) layer. PLG synthesis has been directly linked glutamine synthetase (GS) enzyme. glnA1 gene encodes for GS enzyme in mycobacteria. layer absent avirulent Mycobacterium smegmatis , although M. strain expressing can synthesize the wall. role extensively studied tuberculosis however, little about other mycobacterial species. bovis, as an intracellular pathogen encounters nitrogen stress inside...
Abstract Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), initially originated in China year 2019 and spread rapidly across the globe within 5 months, causing over 96 million cases of infection deaths. Huge efforts were undertaken to bring COVID-19 vaccines clinical development, so that it can be made available at earliest, if found efficacious trials. We developed a candidate vaccine ZyCoV-D comprising DNA plasmid vector carrying gene encoding spike protein (S) SARS-CoV-2...
A novel Valine coated magnetic nano-particles (MNPs-Val) has been synthesized for the removal of Cd(II) ions from aqueous solution. The MNPs-Val were developed by electrostatic attraction valine (C5H11NO2) on bare surface Fe3O4 nanoparticles and characterized using FT-IR, XRD, SEM, TEM analysis. morphology average particles size 15-27 nm analyzed SEM TEM. MNPs applied adsorptive solutions. Factors affecting adsorption such as pH, temperature, adsorbent dosage, contact time investigated which...
To assess the outcome of unreamed intramedullary nailing through lateralised entry point using oblique proximal and biplanar distal interlocking screws.15 men 3 women aged 25 to 58 (mean, 37) years underwent with screws for third metaphyseal tibial fractures. The was kept tuberosity slightly lateral midline. Proximal locking at 45 degrees coronal sagittal planes. Biplanar in planes.16 patients had bone union within 20 17; range, 14-27) weeks; 2 dynamisation delayed union. Three valgus...
The emerging lung pathogen Mycobacterium abscessus is understudied for its virulence determinants and molecular targets diagnosis therapeutics. Here, we report a comprehensive secretome (600 proteins) of this species, which was identified using multipronged strategy based on genetic/genomic, proteomic, bioinformatic approaches. In-solution digested bottom-up proteomics from various growth phases total 517 proteins, while 2D-GE 33 proteins. A reporter-gene-fusion-based genomic library that...
Background: Occupational exposure to industrial Metalworking Fluid (MWF) colonized by Mycobacterium immunogenum (MI) has been associated with immune lung disease hypersensitivity pneumonitis (HP) in machinists. This warrants regular fluid monitoring for early detection of mycobacterial proteins, especially those antigenic potential. Objective: To detect and identify dominant MI proteins antigens directly from the field-drawn in-use MWF using an integrated immunoproteomic immunoinformatic...
A study dealing with the uptake of zinc-65 by different segments epididymis and vas deferens rhesus monkey (Macaca mulatta) in presence or absence spermatozoa is reported. It was determined that control side significantly higher than epididymal (vas vs. caput, proximal distal corpus, cauda, p less .01). Amon g themselves there no significant difference . Efferent duct ligation consistently reduced all portions (control ligated: vas, The percentage reduction remained virtually constant...