A5100319688- Pancreatic function and diabetes
- Liver Disease and Transplantation
- Organ Transplantation Techniques and Outcomes
- Endoplasmic Reticulum Stress and Disease
- Chronic Kidney Disease and Diabetes
- Cholesterol and Lipid Metabolism
- Hormonal Regulation and Hypertension
- Diabetes and associated disorders
- Kidney Stones and Urolithiasis Treatments
- Chronic Myeloid Leukemia Treatments
- Phytoestrogen effects and research
- Food Quality and Safety Studies
- Caveolin-1 and cellular processes
- Acute Myeloid Leukemia Research
- Pediatric health and respiratory diseases
- Magnesium in Health and Disease
- Acute Lymphoblastic Leukemia research
- Ginseng Biological Effects and Applications
- Hepatitis B Virus Studies
- Anesthesia and Pain Management
- Porphyrin Metabolism and Disorders
- Hereditary Neurological Disorders
- Birth, Development, and Health
- RNA regulation and disease
- Salivary Gland Disorders and Functions
Shenyang Medical College
2019-2025
Liaoning University
2011-2023
Children's Hospital of Fudan University
2023
Weatherford College
2020
Peking University
2020
Center for Life Sciences
2020
Southern Medical University
2017
Lanzhou University
2017
Zhujiang Hospital
2017
Nephrolithiasis is a complicated disease affected by various environmental and genetic factors. Crystal-cell adhesion critical initiation process during kidney stone formation. However, genes regulated factors in this remain unclear. In the present study, we integrated gene expression profile data whole-exome sequencing of patients with calcium stones, found that ATP1A1 might be key susceptibility involved The study showed T-allele rs11540947 5'-untranslated region was associated higher risk...
3β-Hydroxysteroid-Δ24 reductase (DHCR24) is an endoplasmic reticulum (ER)-localized multifunctional enzyme that possesses anti-apoptotic and cholesterol-synthesizing activities. Accumulating evidence suggests ER stress involved in the pathogenesis of neurodegenerative disease. In this study, we investigated whether DHCR24 may function as a neuroprotective protein under stress. Neuroblastoma N2A cells were infected with adenovirus expressing myc-tagged (Ad-DHCR24) or lacZ (Ad-lacZ, serving...
Hyperlipidemia is a risk factor for the development of fatty liver and cardiovascular diseases such as atherosclerosis coronary heart disease, hence, cholesterol-lowering drugs are considered important effective in preventing diseases. Thus, researchers field new drug endeavoring to identify types drugs. 3β-hydroxysterol-Δ(24)-reductase (DHCR24) catalyzes conversion desmosterol cholesterol, which last step cholesterol biosynthesis pathway. We speculated that blocking catalytic activity...
The streptozotocin (STZ) model is widely used in diabetes research. However, the cellular and molecular states of pancreatic endocrine cells this remain unclear. This study explored characteristics islet treated with STZ re-evaluated β-cell dysfunction regeneration model. We performed single-cell RNA sequencing from STZ-treated mice. High-quality data 2,999 were to identify clusters via Louvain clustering analysis. Principal component analysis (PCA), t-distributed stochastic neighbor...
Crystal-cell interactions are a vital step toward kidney stone formation. However, its mechanisms remained unclear. Here, protein-protein interaction (PPI) network analysis of revealed that the proteins were enriched in posttranslational protein modification process endoplasmic reticulum (ER). The vitro study showed markers ER stress, including Bip and CHOP, upregulated, PERK ATF6 activated, XBP-1 mRNA was spliced. An stress-specific protein, caspase-12, activated apoptotic cells induced by...
Bioartificial liver holds special position in the field of regenerative medicine, and cold environment at 4℃ is widely used for short storage both organ cell later application. However, disadvantages such could influence viability lead to apoptosis different degrees. In this study, we mainly explore pre-protective effect mild hypothermia against low temperature (4℃)-induced rat injury vitro. Our results indicated that precondition with increase viability, as proliferation, LDH regulation...
There is accumulating evidence showing that apoptosis induced by endoplasmic reticulum (ER) stress plays a key role in pancreatic β cell dysfunction and insulin resistance. 3 -Hydroxysteroid- Δ 24 Reductase (DHCR24) multifunctional enzyme located the (ER), which has been previously shown to protect neuronal cells from ER stress-induced apoptosis. However, of DHCR24 type 2 diabetes only incompletely understood so far. In present study, we tunicamycin (TM) treatment showed infection MIN6 with...
Some relationship between abnormal cholesterol content and impairment of insulin/insulin-like growth factor I (IGF-1) signaling has been reported in the pathogenesis Alzheimer's disease (AD). However, underlying mechanism this correlation remains unclear. It is known that 3-β hydroxycholesterol Δ 24 reductase (DHCR24) catalyzes last step biosynthesis. To explore function AD, we depleted cellular by targeting DHCR24 with siRNA (siDHCR24) or U18666A, an inhibitor DHCR24, studied effect loss on...
The growing evidences demonstrated hyperlipidemia in obesity and type 2 diabetes is characterized by high levels of free fatty acids, low-density lipoprotein (LDL), triglyceride, cholesterol. We investigated the effect LDL particles (LDLs) loading on MIN6 cells derived from pancreatic β cells. Exposure to LDLs induced apoptosis dose time-dependent manner, TUNEL situ apoptotic assay. immunocytochemical analysis Western blotting revealed that LDLs-induced associated with activation caspase 3...
Hepatic ischemia-reperfusion injury is one of the most important factors for prognosis liver transplantation and hepatic surgery. It was reported that glycogen synthase kinase-3 (GSK-3) regulated response during ischemia-reperfusion. In this study, we investigated protective effects GSK-3 inhibitor CHIR99021 against injury. A H2O2-induced oxidative model using LO2 cells established. were divided into four groups, including blank control group, group treated with alone, H2O2-injury H2O2...
Background: Kawasaki disease (KD) is an acute vasculitis, that is, the leading cause of acquired heart in children, with approximately 10%–20% patients KD suffering intravenous immunoglobulin (IVIG) resistance. Although underlying mechanism this phenomenon remains unclear, recent studies have revealed immune cell infiltration may associate its occurrence. Methods: In study, we downloaded expression profiles from GSE48498 and GSE16797 datasets Gene Expression Omnibus database, analyzed...
Abstract Since the outbreak of novel Corona Virus Disease 2019 (COVID-19) infected by SARS-CoV-2 at end 2019, clinical specific antiviral drugs have been lacking. A Chinese patent medicine called ‘Toujiequwen Granules’ has promoted in treatment COVID-19. The present study was designed to reveal molecular mechanism Toujiequwen Granules against network pharmacological method applied screen main active ingredients Tongjiequwen Granules. Network analysis 149 and 330 drug targets showed most...