PIF3 is a phytochrome-interacting basic helix-loop-helix transcription factor that negatively regulates light responses, including hypocotyl elongation, cotyledon opening, and negative gravitropism. However, the role of in chlorophyll biosynthesis has not been clearly defined. Here, we show also by repressing biosynthetic genes dark. Consistent with gene expression patterns, etiolated pif3 mutant accumulated higher amount protochlorophyllide was bleached severely when transferred into light....

Summary Phytochromes are red/far‐red light receptors that regulate various responses by initiating the transcriptional cascades responsible for changing expression patterns of 10–30% entire plant transcriptome. Several transcription factors thought to participate in this process have been identified, but functional relationships among them not yet fully elucidated. Here we investigated relationship between two such factors, PIF3 and HY5, their effects on anthocyanin biosynthesis. Our results...

Plant photoreceptors regulate various developmental processes. Among the photoreceptors, phytochromes, red and far-red light receptors, responses through many signaling components, including phytochrome-interacting proteins. The functional relationships among phytochromes their interacting proteins, however, have not been clearly established. Here, we sought to identify a relationship between phytochrome factor 3 (PIF3). We demonstrate that PIF3 is polyubiquitinated rapidly subsequently...

We surveyed differential gene expression patterns during early photomorphogenesis in both wild-type and mutant Arabidopsis defective HY5, an influential positive regulator of the responses to a light stimulus, identify light-responsive genes whose was HY5 dependent. These gene-expression data identified light-regulated zinc finger protein 1 (LZF1), encoding previously uncharacterized C2C2-CO B-box transcriptional regulator. has trans-activating activity toward LZF1 binding affinity promoter...

Plants are confronted with predictable daily biotic and abiotic stresses that result from the day–night cycle. The circadian clock provides an anticipation mechanism to respond these stress signals increase fitness. Jasmonate (JA) is a phytohormone mediates various growth responses. Here, we found circadian-clock component TIME FOR COFFEE (TIC) acts as negative factor in JA-signaling pathway. We showed tic mutant hypersensitive growth-repressive effects of JA displays altered JA-regulated...

Developmental endothelial locus–1 (DEL-1) is an endogenous homeostatic regulator of osteoclasts that blocks inflammatory bone loss in nonhuman primates.

Phytochromes are red and far-red light photoreceptors that regulate various aspects of plant development. One the less-understood roles phytochromes is inhibition hypocotyl negative gravitropism, which refers to loss gravitropism resulting random growth direction in or light. This response allows seedlings curve toward blue after emergence from soil enhances seedling establishment presence mulch. inhibit by inhibiting four phytochrome-interacting factors (PIF1, PIF3, PIF4, PIF5), as shown...

Abstract Background Rheumatoid arthritis (RA) is an autoimmune disease of inflammatory joint damage, wherein C-reactive protein and autoantibodies including rheumatoid factor (RF) anti-cyclic citrullinated peptide (anti-CCP) are rapidly elevated. These serological factors diagnostic markers RA; however, their sensitivity specificity for prediction warrant improvement early accurate diagnosis. Methods We aimed to identify alternative biomarkers by serum profiling using LC-MS/MS. performed...

Summary Plants often respond to environmental changes by reprogramming metabolic and stress‐associated pathways. Homeostatic integration of signaling is a central requirement for ensuring stability in living organisms. Under diurnal conditions, properly timed rhythmic metabolism provides fitness benefits plants. TIME FOR COFFEE ( TIC ) circadian regulator known be involved clock resetting at dawn. Here we explored the mechanism influence plant growth development, as initiated microarray...

Plants generate rhythmic metabolism during the repetitive day/night cycle. The circadian clock produces internal biological rhythms to synchronize numerous metabolic processes such that they occur at required time of day. Metabolism conversely influences function by controlling period and phase expression core-clock genes. Here, we show AKIN10, a catalytic subunit evolutionarily conserved key energy sensor sucrose non-fermenting 1 (Snf1)-related kinase (SnRK1) complex, plays an important...

Abstract Objectives Histone deacetylase (HDAC) 6 promotes inflammation. We investigated the anti-arthritic effects of CKD-506, a novel HDAC6 inhibitor, in vitro and murine model arthritis as treatment option for rheumatoid (RA). Methods was overexpressed mouse peritoneal macrophages RAW 264.7 cells, inhibitor CKD-506 on cytokine production activity NF-κB AP-1 signaling were examined. Peripheral blood mononuclear cells (PBMCs) from RA patients fibroblast-like synoviocytes (FLS) activated...

Cryptochromes (CRY) are one of the two major classes photoreceptors that perceive light stimuli in UV-A to blue region and they involved multiple aspects plant growth development. However, knowledge regarding their signaling transduction components mechanisms remains limited. Here, we report a MYB transcription factor Blue Insensitive Trait 1 (BIT1), plays an important role controlling responses. Hypocotyl responses indicate BIT1 functions as positive element signaling, since antisense...

Abstract The glycoprotein milk fat globule–epidermal growth factor 8 (MFG-E8) is expressed in several tissues and mediates diverse homeostatic functions. However, whether it plays a role bone homeostasis has not been established. In this study, we show for the first time, to our knowledge, that osteoclasts express are regulated by MFG-E8. Bone marrow–derived osteoclast precursors from MFG-E8–deficient (Mfge8−/−) mice underwent increased receptor activator of NF-κB ligand–induced...

AbstractVascular endothelial growth factor (VEGF) activates the VEGF-VEGF receptor (VEGFR) signaling pathway in angiogenesis. Some cancer cell lines show decreased expression of two VEGFRs, Flt-1 and KDR, even though VEGF is uniformly expressed lines. Promoter methylation a well-known cause epigenetic gene silencing cells. Although VEGF, KDR have typical CpG islands their promoter regions, transcriptional alterations these genes not yet been described. The present study evaluated VEGFR...

Developmental endothelial locus-1 (Del-1) is an cell-secreted protein that limits the recruitment of neutrophils by antagonizing interaction between LFA-1 integrin on and intercellular adhesion molecule (ICAM)-1 cells. Mice with genetic or age-associated Del-1 deficiency exhibit increased neutrophil infiltration in periodontium resulting inflammatory bone loss. Here we investigated additional novel mechanisms whereby could interfere inflammation. Treatment human cells did not affect...

The purpose of this study is to confirm the health behavior performance rate and cardiovascular disease-related indicators according subjective perception prediabetic diabetic patients using 2016-2019 National Health Nutrition Examination Survey (NHANES) data. This classified hemoglobin A1c ≥ 6.5% as diabetes 5.7-6.4% prediabetes among 2485 adults over 40 years age data from Survey. In addition, was divided into 'good' 'bad' then cross-classified four groups (Good/PDM, Bad/PDM, Good/DM,...

We evaluated whether the inhibitory effects of vascular endothelial growth factor (VEGF)-targeted drugs on proliferation cancer cells differed according to VEGF receptor (VEGFR) genes, Flt1 and KDR, promoter methylation status. Five hyper-VEGFR-methylation six no-VEGFR-methylation were used for present study, together with human umbilical (HUVECs) as a control. No-VEGFR-methylation showed higher expression KDR than cells. Hyper-VEGFR-methylation only increased protein levels after treatment...

Abstract Background To investigate the effects of inhibiting histone deacetylase (HDAC) 6 on inflammatory responses and tissue-destructive functions fibroblast-like synoviocytes (FLS) in rheumatoid arthritis (RA). Methods FLS from RA patients were activated with interleukin (IL)-1β presence increasing concentrations M808, a novel specific HDAC6 inhibitor. Production ILs, chemokines, metalloproteinases (MMPs) was measured ELISAs. Acetylation tubulin expression ICAM-1 VCAM-1 assessed by...

Abstract The Arabidopsis HY5 protein is a basic leucine zipper (bZIP) transcription factor that promotes photomorphogenesis. binds directly to the promoters of light responsible element containing G‐box and thus regulates their transcriptional activity. level activity are negatively regulated, in light‐dependent manner, by interaction with COP1 protein, which targets for proteasome‐mediated degradation nucleus. Despite its essential roles plant development, no structural information exists...

The secreted protein developmental endothelial locus 1 (DEL-1) regulates inflammatory cell recruitment and protects against pathologies in animal models. Here, we investigated DEL-1 arthritis using collagen-induced (CIA) collagen Ab-induced (CAIA) In both models, mice with endothelium-specific overexpression of were protected from relative to WT controls, whereas was exacerbated DEL-1-deficient mice. Compared VI promoter-driven mesenchymal cells CIA but not CAIA, suggesting a role for the...