Codelivery of multiple drugs with one kind drug carriers provided a promising strategy to suppress the resistance and achieve synergistic therapeutic effect in cancer treatment. In this paper, we successfully developed both methotrexate (MTX) mitomycin C (MMC) loaded PEGylated chitosan nanoparticles (CS-NPs) as delivery systems, which MTX, folic acid analogue, was also employed tumor-targeting ligand. The new systems can coordinate early phase targeting late-phase anticancer effect....

Most present drug-phospholipid delivery systems were based on a water-insoluble complex but rarely water-soluble complex. Mitomycin C (MMC) is anticancer drug extensively used in first-line chemotherapy limited by its poor aqueous stability vitro, rapid elimination from the body, and lack of target specificity. In this article, we report MMC-soybean phosphatidylcholine complex-loaded PEG-lipid-PLA hybrid nanoparticles (NPs) with Folate (FA) functionalization (FA-PEG-PE-PLA NPs@MMC-SPC) for...

Integrating advantages of mitomycin C (MMC)–phospholipid complex for increased drug encapsulation efficiency and reduced premature release, DSPE-PEG-folate (DSPE-PEG-FA) specific tumor targeting, we reported a simple one-pot self-assembly route to prepare the MMC–phospholipid complex-loaded DSPE-PEG-based nanoparticles (MP-PEG-FA NPs). Both confocal imaging flow cytometry demonstrated that MMC was distributed into nuclei after cellular uptake intracellular delivery. More importantly,...

Both folic acid (FA)- and methoxypoly(ethylene glycol) (mPEG)-conjugated chitosan nanoparticles (NPs) had been designed for targeted prolong anticancer drug delivery system. The NPs were prepared with combination of ionic gelation chemical cross-linking method, followed by conjugation both FA mPEG, respectively. FA-mPEG-NPs compared either or mPEG-/FA-NPs in terms their size, targeting cellular efficiency tumor tissue distribution. specificity the mPEG-FA-NPs cancerous cells was demonstrated...

We constructed 10-hydroxycamptothecin (CPT) "nanodrugs" with functionalization of lipid-PEG-methotrexate (MTX) to prepare high-drug-loaded, and sustained/controlled-release MTX-PEG-CPT nanorods (NRs), in which MTX drug itself can serve as a specific "targeting ligand". The self-targeted nanodrug codeliver both CPT drugs distinct anticancer mechanisms. Furthermore, NRs significantly reduced burst release, improved blood circulation tumor accumulation, enhanced cellular uptake, synergistically...

A mitomycin C (MMC)-soybean phosphatidyhlcholine complex loaded in phytosomes was previously reported for the purpose of developing a MMC drug delivery system (Mol. Pharmaceutics 2013, 10, 90-101), but this approach limited by rapid elimination from body and lack target specificity. In article, to overcome these limitations, MMC-soybean complex-loaded (MMC-loaded phytosomes) as carriers were surface-functionalized with folate-PEG (FA-PEG) achieve reduced toxicity superior MMC-mediated...

In the last decade, biosynthesis of metal nanoparticles using organisms have received more and considerations. However, complex composition adds up to a great barrier for characterization biomolecules involved in synthesis process their biological mechanisms.In this research, we biosynthesized kind flower-shaped Au nanoclusters (Au NCs) one definite component-epigallocatechin gallate (EGCG), which was main green tea polyphenols. Possessing good stability 6 weeks size 50 nm, NCs might be...

Mitomycin C is one of the most effective chemotherapeutic agents for a wide spectrum cancers, but its clinical use still hindered by mitomycin (MMC) delivery systems. In this study, MMC-loaded polymer-lipid hybrid nanoparticles (NPs) were prepared single-step assembly (ACS Nano 2012, 6:4955 to 4965) MMC-soybean phosphatidyhlcholine (SPC) complex (Mol. Pharmaceutics 2013, 10:90 101) and biodegradable polylactic acid (PLA) polymers intravenous MMC delivery. The advantage MMC-SPC on NPs was...

Exploration of efficient dual-drug nanohybrids, particularly those with high drug loading, specific targeting property, and long-termed stability, is highly importance in cancer therapy. A pH-driven coprecipitation was performed the aqueous phase to obtain a nanoformulation, composed 10-hydroxycamptothecine (HCPT) nanoneedles integrated an exterior thin layer methotrexate (MTX)–chitosan conjugate. The stability nanohybrids water property provided by MTX ingredient function synergistically...

The particle shape of the drug delivery systems had a strong impact on their in vitro and vivo performance, but there was limited availability techniques to produce specific shaped carriers. In this article, novel methotrexate (MTX) decorated MPEG-PLA nanobacillus (MPEG-PLA-MTX NB) prepared by self-assembly technique followed extrusion through SPG membrane with high N2 pressure for targeted delivery, which Janus-like MTX not only used as anticancer could also be served tumor-targeting...

We present a dialysis technique to direct the self-assembly of paclitaxel (PTX)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(d,l-lactide) (MPEG-PLA) and PLA, respectively. The composition, morphology, particle size zeta potential, drug loading content, encapsulation efficiency both PTX-PLA NPs PTX-MPEG-PLA were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, dynamic light scattering, electrophoretic...

Abstract Recently, methotrexate (MTX) has been used to target folate (FA) receptor-overexpressing cancer cells for targeted drug delivery. However, the systematic evaluation of MTX as a Janus-like agent not reported before. Here, we explored validity using playing an early-phase cancer-specific targeting ligand cooperated with late-phase therapeutic anticancer based on PEGylated chitosan (CS) nanoparticles (NPs) carriers. Some advantages these nanoscaled delivery systems are follows: (1) NPs...

Leading to more and deaths disabilities, stroke has become a serious threat human health. What's more, few effective drugs are available in clinic till now.In this research, we prepared novel neuroprotective nanoformation (OEA-SPC NPs) via the combination of nanoparticle drug delivery system with endogenous N-oleoylethanolamine (OEA). By forming hydrogen bond between OEA carrier-soybean phosphatidylcholine (SPC), form was turned into amorphus state when loading nanoparticles, which greatly...

Since the anticancer drugs have diverse inhibited mechanisms to cancer cells, use of two or more kinds agents may achieve excellent therapeutic effects, especially drug-resistant tumors.In this study, we developed a kind dual drug [methotrexate (MTX) and 10-hydroxycamptothecine (HCPT)] loaded nanoneedles (DDNDs) with pronounced targeting property, high loading prolonged release. The anti-solvent precipitation HCPT MTX modified PEG-b-PLGA (PEG-b-PLGA-MTX, PPMTX) leads nucleation...

A bio-inspired approach was used to integrate an anti-tumor drug into nanocrystalline assemblies for sustained release.

Polymer-controlled crystallization is becoming an increasingly important approach to achieve functional materials precipitated from the solution phase. Nevertheless, there exist multiple pathways under control of yet unpredictable kinetic factors, which significantly hinder mechanistic understanding. Herein, study polymer-controlled precipitation a typical drug compound, indomethacin, was performed. The presence nonionic additive poly(vinylpyrrolidone) induces heterogeneous nucleation...

Although the preparation of lipid nanoparticles (LNPs) achieves great success, their retention highly hydrophobic drugs is still problematic.Herein, we report a novel strategy for efficiently loading to LNPs stroke therapy. Oleoylethanolamide (OEA), an endogenous molecule with outstanding neuroprotective effect, was successfully loaded OEA-SPC&DSPE-PEG (OSDP LNPs) drug 15.9 ± 1.2 wt%. Efficient in OSDP greatly improved pharmaceutical property and enhanced effect OEA.Through data positron...

Polymer–lipid hybrid micelles co-delivered hydrophilic mitomycin C and hydrophobic 10-hydroxycamptothecin showed improved cellular uptake cytotoxicity <italic>in vitro</italic> enhanced tumor accumulation antitumor activity vivo</italic>.

The high-aspect-ratio nanoparticles were proved to be internalized much more rapidly and efficiently by cancer cells than the with an equal aspect ratio. Herein, a kind of high-aspect ratio, pointed-end nanoneedles (NDs) high drug loading (15.04 %) prolonged release profile fabricated anti-tumor drug—10-hydroxycamptothecin (HCPT)—via ultrasound-assisted emulsion crystallization technique. It is surprising see that cellular internalization NDs average length 5 μm ratio about 12:1 was even...