A novel formulation system of phytosomes loaded with mitomycin C-soybean phosphatidylcholine (MMC-SPC) complex (MMC-loaded phytosomes) was prepared by a solvent evaporation method combined nanoprecipitation technique for the purpose development an MMC drug delivery system. The MMC-loaded were evaluated average particle size, zeta-potential, and residual drug-loading content as well in vitro release profile. Furthermore, stability tests vitro/vivo biological evaluations performed. DSC, FTIR,...

Regular exercise maintains a healthy metabolic profile, while the underlying mechanisms have not been fully elucidated. Extracellular vesicles serve as an important mediator in intercellular communication. In this study, we aimed to explore whether exercise-induced extracellular (EVs) of skeletal muscle origins contribute exercise-related protective effects on metabolism. We found that twelve weeks swimming training improved glucose tolerance, reduced visceral lipid accumulation, alleviated...

Both folic acid (FA)- and methoxypoly(ethylene glycol) (mPEG)-conjugated chitosan nanoparticles (NPs) had been designed for targeted prolong anticancer drug delivery system. The NPs were prepared with combination of ionic gelation chemical cross-linking method, followed by conjugation both FA mPEG, respectively. FA-mPEG-NPs compared either or mPEG-/FA-NPs in terms their size, targeting cellular efficiency tumor tissue distribution. specificity the mPEG-FA-NPs cancerous cells was demonstrated...

At present, there are few reports on the glycosylation modification of soybean proteins under irradiation. In this paper, a protein isolate and maltose were used as raw materials to prepare glycosylated gamma-ray treatment improve functional properties evaluate changes in structure. The results analysis graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum ultraviolet modified product showed that Maillard reaction between occurred...

Extracellular vesicles (EV)-based delivery of therapeutic mRNAs is challenged by the low loading efficiency. In this study, we designed a DNA aptamer consisting two parts: single strand part recognized AUG region target mRNA, preventing mRNA from translation and ribosome assembly; double containing elements CD9-ZF (zinc finger) motifs, sorting aptamer-mRNA complex into engineered EVs. vitro in vivo studies revealed that system could efficiently load functional to Furthermore, adipose...

Trehalose is an important carbohydrate substance in insect hemolymph. Chitin the main component of cuticle and peritrophic matrix insects. Trehalase (Tre) catalyzes decomposition trehalose. Few studies trehalase lepidopteran insects have been conducted. Here, functions soluble Tre (Tre1) membrane-bound (Tre2) growth development Mythimna separata were investigated. We cloned identified Tre1 Tre2 cDNA sequences M. separata. Analysis expression revealed that MsTre1 MsTre2 highly expressed...

Krüppel-like Factor 9 (KLF9) is a transcription factor that regulates multiple disease processes. Studies have focused on the role of KLF9 in redox system. In this study, we aimed to explore effect diabetic cardiomyopathy.Cardiac-specific overexpression or silencing C57BL/6 J mice was induced with an adeno-associated virus (AAV9) delivery Mice were also subjected streptozotocin injection establish cardiomyopathy model. addition, neonatal rat cardiomyocytes used assess possible vitro by...

Abstract Background: Personalized therapy is an emerging approach in cancer treatment. Melanoma, known for its poor prognosis, the deadliest form of skin cancer, with current treatments lacking precise guidance individualized care. Methods: We conducted analysis RNA-seq and microarray data obtained from TCGA GEO databases, alongside single-cell RNA sequencing (scRNA-seq) glioma patients within repository. This comprehensive investigation, augmented by experimental studies, concentrated on...

The significantly rising incidence of oral cancer worldwide urgently requires the identification novel, effective molecular targets to inhibit progression malignancy. DNA topoisomerase I (Topo I) is a well-established target for treatment, and many studies have shown that different cell genes could be targeted more selectively with one type Topo inhibitor. In this report, new scaffold pyridothieno[3,2-c]isoquinoline 11,11-dioxide was designed via combination key fragment or bioisoster...

In this study, tea polyphenols (TP) was added to a soy protein isolate (SPI) prepare nanoemulsions by ultra-high pressure homogenization (UHPH). The were characterized confocal laser scanning electron microscopy, infrared spectroscopy, dynamic rheometer and size-potential analyzer. effects of TP on the hydrophobicity, emulsifiability, particle size, potential antioxidant capacity prepared investigated. properties with different concentrations analyzed. results indicated that treatment...

Cytochrome P450 (CYP) is a group of important detoxification enzymes found in insects related to their resistance insecticides. To elucidate the CYP6 family genes P450, which are potentially imidacloprid

The estimation of yeast viability with B- and N-doped carbon dots (BN-CDs) was investigated in this paper. BN-CDs a fluorescent quantum yield 65.47% were prepared by one-step hydrothermal method. size distribution relatively narrow, the majority falling within 7.5-8.5 nm, they mainly composed carbon, oxygen, nitrogen, boron. shown to have strong stable fluorescence. They exhibited excitation-independent photoluminescence property, which could avoid autofluorescence limitation excitation...

In this work, the effect of inorganic–organic corrosion inhibitor composition on inhibition rate was studied through electrochemical accelerated experiments and ambient atmosphere experiments. The performance analyzed to establish relationship between composition, steel temperature, treatment time rate. nano-emulsion type reinforcing shows that different types nano-emulsions can increase self-corrosion potential reduce current steel, improving resistance steel. highest 72.1% could be...

Blue cone monochromacy (BCM) is a congenital vision disorder affecting both middle-wavelength (M) and long-wavelength (L) photoreceptors of the human retina. BCM results from abolished expression green red light-sensitive visual pigments expressed in M- L-cones, respectively. Previously, we showed that gene augmentation therapy to deliver either L- or M-opsin rescues dorsal dominant structurally functionally treated knockout (Opn1mw−/−) mice. Although Opn1mw−/− mice represent disease model...

Arterial stiffness due to the vessel remodeling is closely linked raised blood pressure, and its physiopathologic mechanism still not fully understood. We here aimed explore whether extracellular vesicle (EV) mediated intercellular communication between endothelium smooth muscle cell contribute under hypertension. revealed that arterial endothelial cells robustly secreted EV, which in turn could be circulated and/or directly taken up by subendothelial (SMC). Under hypertension, EV secretion...

To explore the traumatic endophthalmitis in young children and outcome of pars plana vitrectomy (PPV).Twenty-two eyes 22 cases consecutive pediatric treated followed up between September 2014 May 2018 were included. Aqueous humor or vitreous samples taken for bacterial culture sensitivity tests. Intravitreal antibiotics (norvancomycin ceftazidime) injection, combined with 23-gauge PPV, administered eyes. Silicone oil (SO; 5000 centistoke) tamponade perfluoropropane gas (C3F8) was used all...

Background: The relationship between fruit, whole grain, and total energy consumption the gut microbiome in Chinese population remains unclear. Objective: We investigated intakes of fruits, grains, energy, diversity composition microbiota. Design: This cross-sectional study included 167 subjects aged 40-75 years who underwent colonoscopy at Nankai Hospital Tianjin, China. Each participants completed a personal history questionnaire, 7-day dietary record, donated fecal sample....

Abstract Hydroxycamptothecin (HCPT)‐loaded PLA nanoparticles were prepared by a one‐step method using the direct dialysis technique, and examined for particle characteristics, in vitro drug release, cytotoxicity, as well antitumor efficiency. Three main influential factors based on results of single‐factor test, i.e., concentration, ratio HCPT to (wt/wt), bags with different molecule weight cutoffs, evaluated an orthogonal design, giving average diameter ∼226.8 nm smooth surface, modest...

Purpose Drawing on the work design growth model (WDGM), this paper aims to explore relationship between job complexity and employee creativity through feedback-seeking moderating effect of team leaders with a creative mindset. Design/methodology/approach The authors used an online survey test hypotheses. Data was collected in three waves from 74 supervisors 349 paired employees China. Findings Job had positive association employees’ feedback-seeking, which further linked creativity. This...