A5101889762- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- Photoacoustic and Ultrasonic Imaging
- Microbial Metabolites in Food Biotechnology
- Food composition and properties
- Advanced biosensing and bioanalysis techniques
- Sexual function and dysfunction studies
- Chemical Synthesis and Analysis
- Ultrasound and Hyperthermia Applications
- Immune Cell Function and Interaction
- Click Chemistry and Applications
- Machine Learning in Materials Science
- Radiopharmaceutical Chemistry and Applications
- Natural Compound Pharmacology Studies
- Antimicrobial Peptides and Activities
- RNA regulation and disease
- Sexuality, Behavior, and Technology
- Plant Stress Responses and Tolerance
- Autophagy in Disease and Therapy
- Tryptophan and brain disorders
- Immune cells in cancer
- Polysaccharides Composition and Applications
- Cancer Treatment and Pharmacology
- Block Copolymer Self-Assembly
Air Force Medical University
2020-2024
Tang Du Hospital
2024
State Key Laboratory of Rice Biology
2023-2024
Zhejiang University
2023-2024
Northeast Forestry University
2022-2023
China National Rice Research Institute
2023
Ministry of Agriculture and Rural Affairs
2023
Shantou University
2021
First Affiliated Hospital of Shantou University Medical College
2021
Xi'an Medical University
2020
Abstract Immunotherapy holds great promise for the treatment of malignant cancer. However, lack sufficient tumor neoantigens and incomplete dendritic cell (DC) maturation compromise efficacy immunotherapy. Here, a modular hydrogel‐based vaccine capable eliciting powerful sustained immune response is developed. Briefly, CCL21a Exo GM‐CSF+Ce6 (tumor cell‐derived exosomes with granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) mRNA encapsulated inside sonosensitizer chlorin e6 (Ce6)...
The identification of new genes involved in regulating cold tolerance rice is urgent because low temperatures repress plant growth and reduce yields. Cold controlled by multiple loci involves a complex regulatory network. Here, we show that jacalin-related lectin (OsJRL) modulates rice. loss OsJRL gene functions increased phenylalanine metabolism flavonoid biosynthesis under stress. knock-out (KO) lines had higher ammonia-lyase (PAL) activity greater accumulation than the wild-type rice,...
Regular exercise maintains a healthy metabolic profile, while the underlying mechanisms have not been fully elucidated. Extracellular vesicles serve as an important mediator in intercellular communication. In this study, we aimed to explore whether exercise-induced extracellular (EVs) of skeletal muscle origins contribute exercise-related protective effects on metabolism. We found that twelve weeks swimming training improved glucose tolerance, reduced visceral lipid accumulation, alleviated...
PDL1 blockade therapy holds great promise in cancer immunotherapy. Ultrasound imaging of expression the tumor is importance predicting therapeutic efficacy. As a proof-of-concept study, novel ultrasound contrast agent has been innovated here to image and block tissue. Briefly, extracellular vesicles (EVs) are engineered display truncated PD1 (tPD1) on surface bind with high affinity by fusion EV-abundant transmembrane protein PTGFRN. The EVs then encapsulated Ca(HCO
Extracellular vesicles (EV)-based delivery of therapeutic mRNAs is challenged by the low loading efficiency. In this study, we designed a DNA aptamer consisting two parts: single strand part recognized AUG region target mRNA, preventing mRNA from translation and ribosome assembly; double containing elements CD9-ZF (zinc finger) motifs, sorting aptamer-mRNA complex into engineered EVs. vitro in vivo studies revealed that system could efficiently load functional to Furthermore, adipose...
Abstract Accurately evaluating tumor neoangiogenesis and conducting precise interventions toward an immune‐favorable microenvironment are of significant clinical importance. In this study, a novel nanodroplet termed as the nanodroplet‐based ultrasound contrast agent therapeutic (NDs UCA/Tx ) is designed for imaging neoangiogenesis. Briefly, NDs shell constructed from engineered CMs containing antigen, vascular endothelial growth factor receptor 1 (VEGFR1) extracellular domain 2–3, CD93...
Immune suppressive microenvironment in tumor emerges as the main obstacle for cancer immunotherapy. In this study, we identified that HIF1α was activated associated macrophages and acted an important factor immune microenvironment. Epigenetically silencing of Hif1α via histone H3 methylation promoter region achieved by CRISPR/dCas9-EZH2 system, which methylase EZH2 recruited to specifically. The silenced macrophage, namely HERM (Hif1α Repressed Macrophage) manifested inheritable suppressing...
Polymeric nanoparticles with multifunctional capabilities, including surface functionalization, hold great promise to address challenges in targeted drug delivery. Here, we describe a concise, robust synthesis of heterofunctional polyethylene glycol (PEG), HO-PEG-azide. This macromer was used synthesize polylactide (PLA)-PEG-azide, functional diblock copolymer. Rapid precipitation this copolymer hydrophobic cargo resulted the generation monodisperse azides corona. To demonstrate conjugation...
Parkinson's disease (PD) is the second most common neurodegenerative disease, while there still no effective way to stop its progress. Early detection crucial for prevention and treatment of disease. However, current diagnosis mainly depends on symptoms, so it necessary establish a reliable imaging modality PD progression monitoring. Others' our previous studies demonstrated that substantia nigra hyperechogenicity (SNH) was detected by transcranial sonography (TCS) in ventral midbrain...
The crosslinking of the core nanoparticles composed polystyrene-block-poly(ethylene oxide) copolymers can be achieved through encapsulation a small molecule aryl diazide, 4,4′-diazidobiphenyl, and subsequent photolysis. core-crosslinked exhibited high stability under thermal challenge. These stabilized have potential to serve as nanobead for polymerase chain reaction (PCR). We also demonstrated that this crosslinker endow thin films polystyrene with solvent resistance. NMR studies on these...
“Stapling” proteins using the copper-catalyzed azide–alkyne click reaction endows these with improved properties, including thermostability and binding affinity.
The content of resistant starch (RS) was considered positively correlated with the apparent amylose (AAC). Here, we analyzed two Indica rice mutants, RS111 and Zhedagaozhi 1B, similar in high AAC found that their RS differed remarkably. had higher RS3 but lower RS2 than 1B; correspondingly, cooked showed slower digestibility. smaller irregular oval granules when compared 1B wild type. a B-type pattern, different from type, which A-type starch. Meantime, more fa fb1 less fb3 1B. Both mutants...
Journal Article Bcl-2 family interactome analysis using bacterial surface display Get access Siyan Zhang, Zhang Department of Chemical and Biological Engineering, A207 Engineering Quadrangle, Princeton University, Princeton, NJ 08544. Fax: (609) 258-0211; Tel: 258-7191 Search for other works by this author on: Oxford Academic Google Scholar A. James Link 258-7191Department Molecular Biology, 08544 E-mail: ajlink@princeton.edu Integrative Volume 3, Issue 8, August 2011, Pages 823–831,...
Engineered aminoacyl-tRNA synthetases have been used to enable the incorporation of many unnatural amino acids into recombinant proteins in vivo. In majority these studies, engineered synthetase is harbored on a plasmid while host retains wild-type copy its genome. Herein, we construct strain carrying single genomic methionyl-tRNA (MetRS) gene, metG*, azidonorleucine (ANL) proteins. The resulting strain, M15MA capable both supporting robust cell growth and enabling production >20 mg/L...
New sequencing technologies based on massively parallel signature (MPSS) have been developed to reduce the cost of genome sequencing. In some current MPSS platforms, DNA-modified micrometer-scale beads are used template polymerase chain reaction (PCR). Reducing size nanoscale can lead significant improvements in throughput. To this end, we assembled polymeric nanobeads that efficiently PCR, resulting DNA-decorated "nanobeads" with a high extent functionalization.
A PHQ-9 score ≥ 15, represented as PHQ-9+ , indicates major depressive disorder (MDD). On using PHQ-9, the psychological burden of several patients with lifelong premature ejaculation (LPE) gets aggravated, which may lead to LPE development. We aim construct a nomogram for predicting individual risk in and discerning those low risks, who should avoid PHQ-9.The was constructed by analysing data 802 from Xijing Hospital Northwest Women's & Children's Hospital. The LASSO multivariable logistic...
At present, increasing importance has been attracted to healthy food enriched in resistant starch (RS), which great benefits health-promoting. Raw potato rich RS2, whereas most RS2 may become digestible after gelatinization, resulting few RS being left processed potato. Breeding potatoes with high or RS3 both can meet the demand for various products.There were apparent discrepancies among three contrast and content thermal properties, viscosity digestibility. ZS-5 had highest 50.17% but...
Abstract Purpose: The relationship between chronic obstructive pulmonary disease (COPD) and hospitalized prognosis, in ischemic stroke patients, as well complications is not understood. This study aimed to investigate the influence of COPD on inpatient outcomes among patients. Methods: A retrospective investigation was conducted 9260 patients with confirmed stroke, First Affiliated Hospital Shantou University Medical College, from 2013 2020. After excluding missing data or hospital discharge...