A5102016389- Enzyme Catalysis and Immobilization
- Protein Hydrolysis and Bioactive Peptides
- Proteins in Food Systems
- Electrochemical sensors and biosensors
- Meat and Animal Product Quality
- Food Chemistry and Fat Analysis
- Food composition and properties
- Analytical Chemistry and Chromatography
- Antioxidant Activity and Oxidative Stress
- Enzyme Production and Characterization
- Mass Spectrometry Techniques and Applications
- Hydrogels: synthesis, properties, applications
- Microencapsulation and Drying Processes
- RNA and protein synthesis mechanisms
- Lipid metabolism and biosynthesis
- Insect Utilization and Effects
- Microbial Metabolism and Applications
- Electrospun Nanofibers in Biomedical Applications
- Catalysis for Biomass Conversion
- Radiation Effects and Dosimetry
- Phytase and its Applications
- Gut microbiota and health
- Pesticide Residue Analysis and Safety
- Carcinogens and Genotoxicity Assessment
- Cholesterol and Lipid Metabolism
Northeast Agricultural University
2012-2025
Hainan University
2025
Science North
2013-2017
Center for Food Safety and Applied Nutrition
2013
In this study, enzymatic hydrolysis was used to fabricate wheat gliadin hydrolysates (WGHs) for the encapsulation and protection of naringin. The exposure hydrophilic amino acids decreased critical micelle concentration (from 0.53 ± 0.02 mg/mL 0.35 0.03 mg/mL) improved solubility, which provided amphiphilic conditions delivery with a degree (DH) 9 % had strongest binding affinity naringin, exhibited smallest particle size (113.7 1.1 nm) highest rate (83.2 1.3 %). storage, heat photochemical...
Abstract To address the unique challenges of diabetic wound healing, dressings, particularly multifunctional hydrogels have garnered considerable interest. For first time, a novel environmentally friendly soy protein‐based hydrogel is developed to accelerate healing chronic wounds. Specifically, this framework in direct formation through dynamic Schiff base between oxidized guar gum and epigallocatechin‐3‐gallate (EGCG)‐modified protein isolate. Meantime, addition Ag + enhances cross‐linking...
In the present study, a novel angiotensin I-converting enzyme inhibitory (ACE inhibitory) peptide, EPNGLLLPQY, derived from walnut seed storage protein, fragment residues 80-89, was identified by ultra-high performance liquid chromatography electrospray ionization quadrupole time of flight mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) protein hydrolysate. The IC50 value peptide 233.178 μM, which determined high method measuring amount hippuric acid (HA) generated ACE decomposition substrate...
At present, there are few reports on the glycosylation modification of soybean proteins under irradiation. In this paper, a protein isolate and maltose were used as raw materials to prepare glycosylated gamma-ray treatment improve functional properties evaluate changes in structure. The results analysis graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum ultraviolet modified product showed that Maillard reaction between occurred...
Phospholipase A1 (PLA1) was immobilized onto magnetic Fe3O4/SiOx-g-P(GMA) nanoparticles. The properties of PLA1 were compared to free in order assess the feasibility utilizing enzyme for degumming soybean oil. process retained 2066.67 μ/g activity with 64.7% immobilization efficiency. Compared process, had a broader pH-activity profile pH 4.5–6.5 and remarkably stable at 45 °C–55 °C 7 h. optimum temperature 60 50 °C, respectively. After 10 cycles through oil 55 still possessed more than 80%...
Biomolecular condensates are key features of intracellular compartmentalization. As the most prominent nuclear condensate in eukaryotes, nucleolus is a layered multiphase liquid-like structure and site ribosome biogenesis. In nucleolus, ribosomal RNAs (rRNAs) transcribed processed, undergoing multiple maturation steps that ultimately result formation small subunit (SSU) large (LSU). However, how rRNA processing coupled to nucleolar organization poorly understood due lack tools precisely...
Walnut proteins and peptides have been reported to great zinc‐carrying activity. In this study, the decapeptide EPNGLLLPQY (WP‐10) derived from walnut protein has prepared figure out zinc binding mechanisms structure–activity relationships. The space‐conformation of peptide converted after being chelated with Zn(II). As secondary structure, β ‐sheet structure turned into α ‐helix ‐turn structure. According atomic absorption spectra analysis, one could bind Zn(II) via four different sites....
High-purity insoluble dietary fiber from okara (okara-HPIDF) is a raw material with potentially positive effect on colon health. However, the mechanisms of are far clear. In this study, okara-HPIDF and low-purity (okara-LPDF) were fed to C57BL/6 mice acute ulcerative colitis induced by DSS. The levels inflammatory factors, bacterial 16S rDNA sequencing, short-chain fatty acids (SCFAs), bioinformatics analyzed colonic tissue status. results showed that intake HPIDF affected proliferation key...
Abstract This paper presents a method for extracting rice bran oil using magnetic immobilized cellulase (MIC) in fluidized bed (MFB). Cellulase was on Fe 3 O 4 /SiO x -g-P (glycydylmethacrylate) with an average grain size of 120 nm. The hydrolyzed MIC combined alkaline protease to extract oil. Under intermittent conditions, the concentration 1.6 mg/g, liquid material ratio 4:1, enzymatic hydrolysis time 150 min, and yield as high 85.6 ± 1.20% at 55 °C. fluid MFB had field strength 0.022 T,...
The nanomagnetic carrier (Fe 3 O 4 @SiO 2 @p(GMA)) was prepared by atom transfer radical polymerization, and then, the free phospholipase C (PLC) immobilized on it proved results of FT-IR analysis. enzyme loading 135.64 mg/g, activity 8560.7 U/g, average particle size 99.86 ± 0.80 nm, specific saturation magnetization 16.00 0.20 emu/g. PLC-Fe @p(GMA) showed highest activities at pH 7.5, tolerance temperature raised to 65°C in soybean lecithin emulsion. Enzymatic degumming with under...
The aim of this study was evaluation stability immobilized phospholipase A2 (PLA2) for soybean oil degumming. Also, the effect reaction time on residual phosphorus levels investigated according to optimum pH and temperature. free PLA2 three demonstrated significant differences in operation conditions. pH, temperature increased upon immobilization different (PLA2-CA, PLA2-CAC PLA2-CAG). Immobilized showed enhanced thermal retained more than 74% relative activity after 1 h incubation at 60°C,...