A5101581029- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Graphene and Nanomaterials Applications
- Advanced Drug Delivery Systems
- Osteoarthritis Treatment and Mechanisms
- Nanoplatforms for cancer theranostics
- Advanced Nanomaterials in Catalysis
- Lipid Membrane Structure and Behavior
- Connective tissue disorders research
- Advanced biosensing and bioanalysis techniques
- Bone Tumor Diagnosis and Treatments
- Dendrimers and Hyperbranched Polymers
- Chemokine receptors and signaling
- Gold and Silver Nanoparticles Synthesis and Applications
- Calcium Carbonate Crystallization and Inhibition
- Nanocluster Synthesis and Applications
- Advancements in Transdermal Drug Delivery
- Bone Tissue Engineering Materials
- Bone Metabolism and Diseases
- Cancer Immunotherapy and Biomarkers
- Protease and Inhibitor Mechanisms
- Lysosomal Storage Disorders Research
- Metastasis and carcinoma case studies
- Fibroblast Growth Factor Research
Fujian Medical University
2024
Union Hospital
2024
Wayne State University
2020-2024
First Affiliated Hospital of Hebei Medical University
2024
Hebei Medical University
2024
Central South University
2021-2022
Xiangya Hospital Central South University
2021-2022
National Clinical Research
2021
Stomatology Hospital
2021
Liaocheng People's Hospital
2015-2020
Most present drug-phospholipid delivery systems were based on a water-insoluble complex but rarely water-soluble complex. Mitomycin C (MMC) is anticancer drug extensively used in first-line chemotherapy limited by its poor aqueous stability vitro, rapid elimination from the body, and lack of target specificity. In this article, we report MMC-soybean phosphatidylcholine complex-loaded PEG-lipid-PLA hybrid nanoparticles (NPs) with Folate (FA) functionalization (FA-PEG-PE-PLA NPs@MMC-SPC) for...
Abstract Background Due to the lack of research on pathological mechanism temporomandibular joint osteoarthritis (TMJOA), there are few effective treatment measures in clinic. In recent years, microRNAs (miRs) have been demonstrated play an important role pathogenesis (OA) by regulating a variety target genes, and latest evidence shows that miR-21-5p is specifically overexpressed OA. The purpose this project was clarify whether can regulate TMJOA process targeting Spry1. Methods induced...
We constructed 10-hydroxycamptothecin (CPT) "nanodrugs" with functionalization of lipid-PEG-methotrexate (MTX) to prepare high-drug-loaded, and sustained/controlled-release MTX-PEG-CPT nanorods (NRs), in which MTX drug itself can serve as a specific "targeting ligand". The self-targeted nanodrug codeliver both CPT drugs distinct anticancer mechanisms. Furthermore, NRs significantly reduced burst release, improved blood circulation tumor accumulation, enhanced cellular uptake, synergistically...
Abstract Background Due to the unclear pathogenesis of osteoarthritis (OA), effective treatment for this ailment is presently unavailable. Accumulating evidence points chondrocyte senescence as a key driver in OA development. This study aims identify OA-specific microRNAs (miRNAs) targeting alleviate progression. Methods We screened and identified miRNAs differentially expressed normal cartilage, then confirmed impact miR-653-5p on functions phenotypes through vitro experiments with...
In the last decade, biosynthesis of metal nanoparticles using organisms have received more and considerations. However, complex composition adds up to a great barrier for characterization biomolecules involved in synthesis process their biological mechanisms.In this research, we biosynthesized kind flower-shaped Au nanoclusters (Au NCs) one definite component-epigallocatechin gallate (EGCG), which was main green tea polyphenols. Possessing good stability 6 weeks size 50 nm, NCs might be...
Mitomycin C is one of the most effective chemotherapeutic agents for a wide spectrum cancers, but its clinical use still hindered by mitomycin (MMC) delivery systems. In this study, MMC-loaded polymer-lipid hybrid nanoparticles (NPs) were prepared single-step assembly (ACS Nano 2012, 6:4955 to 4965) MMC-soybean phosphatidyhlcholine (SPC) complex (Mol. Pharmaceutics 2013, 10:90 101) and biodegradable polylactic acid (PLA) polymers intravenous MMC delivery. The advantage MMC-SPC on NPs was...
Exploration of efficient dual-drug nanohybrids, particularly those with high drug loading, specific targeting property, and long-termed stability, is highly importance in cancer therapy. A pH-driven coprecipitation was performed the aqueous phase to obtain a nanoformulation, composed 10-hydroxycamptothecine (HCPT) nanoneedles integrated an exterior thin layer methotrexate (MTX)–chitosan conjugate. The stability nanohybrids water property provided by MTX ingredient function synergistically...
The particle shape of the drug delivery systems had a strong impact on their in vitro and vivo performance, but there was limited availability techniques to produce specific shaped carriers. In this article, novel methotrexate (MTX) decorated MPEG-PLA nanobacillus (MPEG-PLA-MTX NB) prepared by self-assembly technique followed extrusion through SPG membrane with high N2 pressure for targeted delivery, which Janus-like MTX not only used as anticancer could also be served tumor-targeting...
Leading to more and deaths disabilities, stroke has become a serious threat human health. What's more, few effective drugs are available in clinic till now.In this research, we prepared novel neuroprotective nanoformation (OEA-SPC NPs) via the combination of nanoparticle drug delivery system with endogenous N-oleoylethanolamine (OEA). By forming hydrogen bond between OEA carrier-soybean phosphatidylcholine (SPC), form was turned into amorphus state when loading nanoparticles, which greatly...
Aims The study aimed to determine whether the microRNA miR21-5p (MiR21) mediates temporomandibular joint osteoarthritis (TMJ-OA) by targeting growth differentiation factor 5 (Gdf5). Methods TMJ-OA was induced in MiR21 knockout (KO) mice and wild-type (WT) a unilateral anterior crossbite (UAC) procedure. Mouse tissues exhibited histopathological changes, as assessed by: Safranin O, toluidine blue, immunohistochemistry staining; western blotting (WB); quantitative real-time polymerase chain...
Background: Mutations in the β-glucocerebrosidase gene (GBA) have been implicated as a risk factor for Parkinson's disease (PD). However, GBA mutations PD patients of different ethnic origins were reported to be inconsistent. Methods: We sequenced all exons 225 and 110 control individuals from Eastern Canada. Result: Two novel variants c.-119 A/G S(-35)N, five known R120W, N370S, L444P, RecNciI RecTL mutation (del55/D409H/RecNciI) well two non-pathological E326K T369M identified while only...
Since the anticancer drugs have diverse inhibited mechanisms to cancer cells, use of two or more kinds agents may achieve excellent therapeutic effects, especially drug-resistant tumors.In this study, we developed a kind dual drug [methotrexate (MTX) and 10-hydroxycamptothecine (HCPT)] loaded nanoneedles (DDNDs) with pronounced targeting property, high loading prolonged release. The anti-solvent precipitation HCPT MTX modified PEG-b-PLGA (PEG-b-PLGA-MTX, PPMTX) leads nucleation...
A bio-inspired approach was used to integrate an anti-tumor drug into nanocrystalline assemblies for sustained release.
Abstract Yin Yang 1 (YY1) regulates gene transcription in a variety of biological processes. In this study, we aim to determine the role YY1 vascular smooth muscle cell (VSMC) phenotypic modulation both vivo and vitro. Here show that injury rodent carotid arteries induces expression along with reduced differentiation markers carotids. Consistent finding, is induced differentiated VSMCs response serum stimulation. To underlying molecular mechanisms, found suppresses CArG box-dependent...
Although the preparation of lipid nanoparticles (LNPs) achieves great success, their retention highly hydrophobic drugs is still problematic.Herein, we report a novel strategy for efficiently loading to LNPs stroke therapy. Oleoylethanolamide (OEA), an endogenous molecule with outstanding neuroprotective effect, was successfully loaded OEA-SPC&DSPE-PEG (OSDP LNPs) drug 15.9 ± 1.2 wt%. Efficient in OSDP greatly improved pharmaceutical property and enhanced effect OEA.Through data positron...
The high-aspect-ratio nanoparticles were proved to be internalized much more rapidly and efficiently by cancer cells than the with an equal aspect ratio. Herein, a kind of high-aspect ratio, pointed-end nanoneedles (NDs) high drug loading (15.04 %) prolonged release profile fabricated anti-tumor drug—10-hydroxycamptothecin (HCPT)—via ultrasound-assisted emulsion crystallization technique. It is surprising see that cellular internalization NDs average length 5 μm ratio about 12:1 was even...
We present a dialysis technique to prepare the 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(D,L-lactide) (PEG-b-PLA) and PLA, respectively. Both HCPT-loaded PEG-b-PLA NPs PLA were characterized by differential scanning calorimetry (DSC), dynamic light scattering (DLS), transmission electron microscopy (TEM), (SEM) confocal laser (CLSM). The results showed that presented hydrodynamic particle size of 120.1 226.8 nm, with polydispersity index...
Causing more and deaths, stroke has been a leading cause of death worldwide. However, success in clinical trials remained elusive. N-oleoylethanolamine (OEA) was an endogenous highly hydrophobic molecule with outstanding neuroprotective effect. In this article, hydrogen bonds were successfully formed between OEA soybean phosphatidylcholine (SPC). The synthetic OEA-SPC complex DSPE-PEG self-assembled into liposomes (OEA NPs), loaded the core PEG hydrophilic shell. Hence, as amorphous state...
Due to the complexity of cancer, combination therapy has been widely used in clinic. The use two kinds anticancer agents with a synergistic effect may achieve excellent therapeutic effects, especially against drug-resistant tumours. In this paper, MTX and HCPT loaded pullulan nanoparticles (MHNPs) were prepared for tumour-targeted therapy. nanoparticles, could serve as both targeting ligand cytotoxic agent. MHNPs possessed size 185.7 ± 16.7 nm sustained release profile dual drug over period...
Since the hydrophobic group is always essential to synthesis of drug-loaded nanoparticles, a majority methods rely heavily on organic solvent, which may not be completely removed and might potential threat patients. In this study, we "green" synthesized 10-hydroxycamptothecine (HCPT) loaded, folate (FA)-modified nanoneedles (HFNDs) for highly efficient cancer therapy with high drug loading, targeting property, imaging capability. It should noted that no solvent was used in preparation...
With high mortality, stroke has become a serious threat to human health. Nevertheless, the strategy for therapy is quite limited in clinic till now. In this research, we prepared novel neuroprotective nanoformulation (OEA Liposomes) via encapsulating endogenous N-oleoylethanolamine (OEA) liposomes intravenous administration. The formulation largely increased solubility and bioavailability of OEA. Then following systematic experiments stated excellent effect OEA Liposomes vivo. survival rate...
The advance of new treatment strategies for more effective management oral cancer requires identification novel biological targets. Therefore, the purpose this study is to identify biomarkers associated with tumorigenesis and prognostic signature by comparing gene expression profile squamous cell carcinomas (OSCCs).Four datasets including GSE25099, GSE30784, GSE37991, GSE41613 were collected from Gene Expression Omnibus (GEO) database. Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG)...