Glioblastoma multiforme (GBM) is the most common primary malignant brain tumor and has a poor prognosis. We, here, report potent antitumor effect of berberine, an isoquinoline alkaloid, on GBM. Berberine was found to have IC50 that much lower than temozolomide in vitro U87, U251, U118 glioblastoma cells. Although previous studies showed berberine primarily exerts its anticancer by inducing cell-cycle arrest, apoptosis, autophagy, we observed cells achieved through induction cellular...

PARP inhibitors have been widely tested in clinical trials, especially for the treatment of breast cancer and ovarian cancer, were shown to be highly successful. Because primarily functions sensing repairing DNA strand breaks, therapeutic effect inhibition is generally believed attributed impaired repair. We here report that oxidative stress also increased by mediates antitumor effect. showed PARP1 expressed specimens high grade serous carcinoma its activity required unperturbed...

Abstract Resveratrol (RSV) acts either as an antioxidant or a pro-oxidant depending on contexts. RSV-treated cancer cells may experience replication stress that can lead to cellular senescence apoptosis. While both oxidative and stresses mediate the anti-proliferation effect of RSV, what extent each contributes impaired proliferation in response RSV remains uncharacterized. We here report study roles mediating treated with RSV. induced S-phase arrest dose-dependent manner U2OS A549 well...

Due to increased glycolysis and poor local perfusion, solid tumors are usually immersed in an acidic microenvironment. While extracellular acidosis is cytotoxic, cancer cells eventually become acclimated it. previous studies have addressed the acute effect of on cells, little known about how survive chronic acidosis. In this study we exposed colorectal (CRC) (HCT15, HCT116 LoVo) pH (pH 6.5) continuously for over three months obtained CRC that pH, designated as CRC-acidosis-acclimated or...

Abstract Activation of p53 may induce apoptosis or cellular senescence in stressed cells. We here report that epidermal growth factor receptor (EGFR) is downregulated by activation a subset cancer cell lines, and this EGFR downregulation mediates caused activation. confers resistance to sustaining the ERK signaling. DYRK1A (dual-specificity tyrosine-phosphorylated tyrosine-regulated kinase 1A), an EGFR-stabilizing kinase, and, when ectopically expressed, can attenuate activation-induced...

Objective To evaluate the risk factors for different types of pregnancy losses after embryo transfer (ET). Design Retrospective cohort study. Setting Reproductive medicine center. Participants A total 15,210 pregnancies fresh and frozen-thawed between January 2014 June 2019. Main Outcome Measures The primary outcome was loss (PL) throughout entire pregnancy. Secondary outcomes were non-visualized PL, early miscarriage, late stillbirth. Methods effect patients’ baseline characteristics...

The recombinase RAD51 catalyzes the DNA strand exchange reaction in homologous recombination (HR) during both mitosis and meiosis. However, physiological role of spermatogenesis remains unclear since null mutation is embryonic lethal mice. In this study, we generated a conditional knockout mouse model to study spermatogenesis. Conditional disruption germ cells by Vasa-Cre led spermatogonial loss Sertoli cell-only syndrome. Furthermore, tamoxifen-inducible UBC-CreERT2 confirmed that deletion...

Abstract Background More than 70% of leiomyomas (LM) harbor MED12 mutations, primarily in exon 2 at c.130-131(GG). The cause mutations myometrial cells remains largely unknown. We hypothesized that increased ROS promotes through the oxidation guanine nucleotides followed by misrepair. Methods Genomic oxidative burden (8-OHdG) was evaluated vitro and vivo immunohistochemistry. were examined Sanger sequencing deep sequencing. Transcriptome RNA-seq performed myometrium with without LM, primary...

Leiomyoma with bizarre nuclei (LM-BN), is a variant of uterine smooth muscle tumor atypical histologic features. Although some LM-BN share several significant genetic alterations leiomyosarcoma, including p16 and p53, the underlying tumorigenesis remains largely unknown. As we previously reported, can be divided into 2 subtypes, type I II, based on different nuclear Type have similar features as tumors fumarate hydratase (FH) alterations. In this study, examined FH expression mutations in 77...

Hypoxia induces a series of cellular adaptive responses that enable promotion inflammation and cancer development. Hypoxia-inducible factor-1α (HIF-1α) is involved in the hypoxia response promotion, it accumulates degraded under normoxic conditions. Here we identify prostate associated transcript-1 (PCAT-1) as hypoxia-inducible long non-coding RNA (lncRNA) regulates HIF-1α stability, crucial for progression. Extensive analyses clinical data indicate PCAT-1 elevated breast patients with...

Polycystic ovary syndrome (PCOS) is a condition in which woman's levels of the sex hormones (estrogen and progesterone) are out balance, leading to growth ovarian cysts. PCOS can affect menstrual cycle, fertility, cardiac function even appearance women. Therefore, we aimed explore genetic polymorphism melatonin receptors 1A 1B obese patients with identify new theoretical basis for its treatment. Patients presenting (n=359) were enrolled classified into an OB‑PCOS group [body mass index (BMI)...

Peanut allergy is becoming a life-threatening disease that could induce severe allergic reactions in modern society, especially for children. The most promising method applied deallergization heating pretreatment. However, the mechanism from view of spectroscopy has not been illustrated. In this study, near-infrared (NIRS) combined with aquaphotomics was introduced to help us understand detailed structural changes information during process. First, (NIR) spectra Ara h1 were acquired 25 80°C....

Objective To evaluate the pregnancy outcomes of progestin-primed ovarian stimulation (PPOS) protocol for patients with endometrioma underwent in vitro fertilization/intra-cytoplasmic sperm injection embryo transfer (IVF/ICSI-ET). Design Observational retrospective cohort study. Setting University affiliated reproductive center. Study Participants 605 infertile IVF/ICSI-ET from January 2016 to March 2021 were included this Methods Multivariable logistic regression analyses conducted determine...

To investigate the effect of two treatments on outcome freeze-thaw embryo transfer for pregnancy assistance in thin endometrium. A retrospective study was conducted 66 patients who failed first cycle treated reproductive medicine center Second Hospital Hebei Medical University from January 2018 to December 2019. Granulocyte colony stimulating factor (G-CSF) used through cavity infusion one group (n=25, and growth hormone (GH) subcutaneously injected (n=41). The clinical data groups were...

The sperm DNA fragmentation index (DFI) is a metric used to assess within sperm. During in vitro fertilization-embryo transfer (IVF-ET), high DFI can lead low fertilization rate, poor embryo development, early miscarriage, etc. A kinase anchoring protein (AKAP) scaffold that bind (PKA) subcellular sites of specific substrates and protects the biophosphorylation reaction. Sperm antigen 17 (SPA17) also AKAP. This study intends explore reason for decreased rate observed (H-DFI) patients during...

Previous studies have indicated a negative correlation between GRK2 expression and pain development transmission. Here, we investigated whether G-protein-coupled receptor kinase 2 (GRK2) was involved in regulating diabetic mechanical hyperalgesia (DMH).The adeno-associated viral vectors containing the gene (AAV-GRK2) were used to up-regulate protein expression. The of exchange directly activated by cyclic adenosine monophosphate 1 (Epac1) dorsal root ganglion (DRG) lumbar 4-6 detected via...