A5056920431- Tryptophan and brain disorders
- Metabolomics and Mass Spectrometry Studies
- Stress Responses and Cortisol
- Advanced Proteomics Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Cancer-related molecular mechanisms research
- Mass Spectrometry Techniques and Applications
- Alzheimer's disease research and treatments
- Circular RNAs in diseases
- Cancer Cells and Metastasis
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Mitochondrial Function and Pathology
- Bioinformatics and Genomic Networks
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- Sleep and Wakefulness Research
- Olfactory and Sensory Function Studies
- Biochemical Acid Research Studies
- Machine Learning in Bioinformatics
- Biotin and Related Studies
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
Chongqing Medical University
2015-2024
Energy Storage Systems (United States)
2022
Beijing University of Chinese Medicine
2022
Zhujiang Hospital
2022
Southern Medical University
2022
Wind Power Engineering (Japan)
2022
Jiangxi Provincial People's Hospital
2021
Southern University of Science and Technology
2021
Nanchang University
2021
Gansu Provincial Hospital of TCM
2019-2020
Abstract Chronic stressful occurrences are documented as a vital cause of both depression and anxiety disorders. However, the stress-induced molecular mechanisms underlying common distinct pathophysiology these disorders remains largely unclear. We utilized chronic mild stress (CMS) rat model to differentiate subgroup depression-susceptible, anxiety-susceptible, insusceptible rats. The hippocampus was analyzed for differential proteomes by combining mass spectrometry isobaric tags relative...
Detergents have been widely used for the solubilization of membrane proteins and improvement their digestion. In this paper, we evaluated application sodium deoxycholate (SDC) to digestion rat hippocampal plasma (PM) proteins. For in-solution digestion, PM fraction from sucrose-density gradient centrifugation was solubilized by boiling in 1.0% SDC, directly digested without dilution. During in-gel separated SDS-PAGE, 0.1% SDC added. Before analysis peptide mixture liquid chromatography...
Major depressive disorder (MDD) is a highly prevalent, debilitating mental illness of importance for global health. However, its molecular pathophysiology remains poorly understood. Combined proteomics and metabolomics approaches should provide comprehensive understanding MDD's etiology. The present study reports novel “-omics” insights from rodent model MDD. Cerebellar samples chronic mild stressed (CMS)-treated depressed rats controls were compared with focus on the differentially...
MicroRNA (miR)‑214 has been demonstrated to suppress gluconeogenesis by targeting activating transcription factor 4 (ATF4), which regulates affecting the transcriptional activity of forkhead box protein O1 (FoxO1). Our previous study revealed that upregulation maternally expressed gene 3 (MEG3), a long noncoding RNA, enhanced hepatic insulin resistance via increased FoxO1 expression. The present aimed explore whether miR‑214 and ATF4 were involved in MEG3‑mediated increase MEG3, expression...
Abstract Chronic stress is a significant risk factor for depression as well anxiety disorders. Yet, the stress-induced specific and common molecular dysregulations of these disorders have not been fully understood. Previously, we constructed chronic mild (CMS) rat model to separate obtain depression-susceptible, anxiety-susceptible, insusceptible groups. In this study, prefrontal cortical proteomes three stressed groups were comparatively profiled utilizing isobaric tags relative absolute...
The hippocampus reconstructs past experiences by integrating sensory, perceptual, and conceptual information across a cortico-hippocampal autobiographical memory network (AMN). Here, in 18 human participants with amnesia, we decoded the effects of bilateral focal hippocampal damage on distinct representations using representational dissimilarity matrices (RDMs). Hippocampal pathology resulted in: (1) impaired generalised episodic retrieval RDM model fit left angular gyrus; and, (2) reduced...
Abstract MicroRNAs (miRNAs) have been reported to play critical roles in the occurrence, progression, and treatment of many cardiovascular diseases. However, molecular mechanism by which miRNA regulates target gene expression ischemia‐reperfusion (I/R) injury acute myocardial infarction (AMI) is not entirely clear. MiR‐340‐5p was be downregulated ischemic stroke. it still remains unknown whether miR‐340‐5p mediated pathogenesis process I/R after AMI. In present study, male C57BL/6 J mice...
Caveolin-1 (Cav-1) was originally identified as a structural protein of caveolae, which is plasma membrane domain that regulates variety signaling pathways involved in cell growth and migration. Here, we show expression Cav-1 the Cav-1-deficient human prostate cancer line LNCaP both stimulates proliferation promotes tumor nude mice. Unexpectedly, expressing (LNCaP(Cav-1)) cells injected into one side mouse promoted negative on contralateral same animal. The tumors were positive for Cav-1,...
Major depressive disorder (MDD) is a highly prevalent mental affecting millions of people worldwide. However, clear causative etiology MDD remains unknown. In this study, we aimed to identify critical protein alterations in plasma from patients with and integrate our proteomics previous metabolomics data reveal significantly perturbed pathways MDD. An isobaric tag for relative absolute quantification (iTRAQ)-based quantitative approach was conducted compare expression between depression...
While stressful events are recognized as an important cause of major depressive disorder, some individuals exposed to life stressors maintain normal psychological functioning. The molecular mechanism(s) underlying this phenomenon remain unclear. Abnormal transmission and plasticity hippocampal synapses have been implied play a key role in the pathoetiology disorder.A chronic mild stress protocol was applied separate susceptible unsusceptible rat subpopulations. Proteomic analysis using...
The enormous depth complexity of the human plasma proteome poses a significant challenge for current mass spectrometry-based proteomic technologies in terms detecting low-level proteins plasma, which is essential successful biomarker discovery efforts. Typically, single-step analytical approach cannot reduce this intrinsic complexity. Current simplex immunodepletion techniques offer limited capacity low-abundance proteins, and integrated strategies are thus desirable. In respect, we...
BACKGROUND Curcumin is a polyphenol compound extracted from the root of herb Curcuma longa, which used in traditional Chinese medicine (TCM). Worldwide, colorectal carcinoma (CRC) an increasing cause morbidity and mortality. This study aimed to investigate effects concentrations curcumin on cell viability, proliferation, apoptosis SW620 human colonic adenocarcinoma cells cultured vitro, signaling pathways involved. MATERIAL AND METHODS were at 0, 4, 8, 16, 32 μmol/l for 48 hours. Specific...
Despite studies providing insight into the neurobiology of chronic stress, depression and anxiety, long noncoding RNA (lncRNA)-mediated mechanisms underlying common distinct pathophysiology these stress-induced disorders remain nonconclusive. In a previous study, we used mild stress paradigm to separate depression-susceptible, anxiety-susceptible insusceptible rat subpopulations. current lncRNA messenger (mRNA) expression was comparatively profiled in hippocampus three groups using...