A5064714800- Transplantation: Methods and Outcomes
- Organ Transplantation Techniques and Outcomes
- Mechanical Circulatory Support Devices
- Mesenchymal stem cell research
- Renal Transplantation Outcomes and Treatments
- Respiratory Support and Mechanisms
- Extracellular vesicles in disease
- Cardiac Ischemia and Reperfusion
- Cardiac Arrest and Resuscitation
- Inhalation and Respiratory Drug Delivery
- Tissue Engineering and Regenerative Medicine
- Neonatal Respiratory Health Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- Respiratory viral infections research
- Immune Response and Inflammation
- Abdominal Surgery and Complications
- Organ Donation and Transplantation
- Microbial infections and disease research
- MicroRNA in disease regulation
- Pneumocystis jirovecii pneumonia detection and treatment
- Antifungal resistance and susceptibility
- Cardiac Structural Anomalies and Repair
- Boron Compounds in Chemistry
Lund University
2020-2025
Johnson University
2020-2025
Rutgers, The State University of New Jersey
2020-2025
New York University
2024-2025
Skåne University Hospital
2022-2025
Robert Wood Johnson University Hospital
2024
Wallenberg Wood Science Center
2024
Despite improvements, lung transplantation remains hampered by both a scarcity of donor organs and mortality following primary graft dysfunction (PGD). Since acute respiratory distress syndrome (ARDS) limits lungs utilization, we investigated cytokine adsorption as means treating ARDS lungs. We induced mild to moderate using lipopolysaccharide in 16 pigs. Lungs were then treated with or without during ex vivo perfusion (EVLP) and/or post-transplantation extracorporeal hemoperfusion. The...
Abstract Acute lung injury (ALI) represents an aetiologically diverse form of pulmonary damage. Part the assessment and diagnosis ALI depends on skilled observer-based scoring brightfield microscopy tissue sections. Although this readout is sufficient to determine gross alterations in structure, its categorical scores lack sensitivity describe more subtle changes morphology. To generate a sensitive alveolar perturbation we carried out high resolution immunofluorescence imaging 200 μm...
Elevated levels of neutrophil extracellular traps (NETs) have been reported in primary graft dysfunction, making methods to reduce or remove them highly valuable. The mechanisms behind dysfunction (PGD) remain rudimentarily understood but its relation higher rates acute and chronic rejection necessitates the development preventative treatments. This case series explores use a cytokine adsorber during lung transplantation with focus reducing circulating nucleosome as measure NETs. Treated...
IntroductionLong-term survival following lung transplantation (LTx) faces impediments due to chronic allograft dysfunction (CLAD), while infections hinder short-term survival. Fungal colonization and invasive fungal (IFI) are common within the first year after LTx. There is ongoing debate regarding impact of such events on CLAD development mortality. This study aims investigate this matter further.MethodsA total 134 LTx recipients transplanted between 2011 – 2020 were included. The median...
Background. Lung transplantation remains hampered by a scarcity of viable donor lungs, partially attributed to lung injuries. Methods. Three porcine injury models were studied: infection-induced using lipopolysaccharide (n = 7), aspiration-induced endotracheal gastric content and lavage harmful ventilation (ventilator-induced injury; n 7). Molecular functional changes from before after the establishment examined with histopathology, immunohistochemistry, cytokine levels, hemodynamics, mass...
Precision-cut lung slices (PCLS) have gained increasing interest as a model to study biology/disease and screening novel therapeutics. In particular, PCLS derived from human tissue can better recapitulate some aspects of compared with animal models. Several experimental readouts been established for use PCLS, but obtaining high-yield -quality RNA downstream analysis has remained challenging. This is particularly problematic utilizing the power next-generation sequencing techniques, such...
Ischemia-reperfusion injury (IRI) plays a crucial role in the development of primary graft dysfunction (PGD) following lung transplantation. A promising novel approach to optimize donor organs before transplantation and reduce incidence PGD is mitochondrial In this study, we explored delivery isolated mitochondria 4 hour ex vivo perfusion (EVLP) as means mitigate IRI. To provide fresh viable source mitochondria, well streamline workflow without need for muscle biopsies, investigated impact...
Lung transplantation is hindered by low donor lung utilization rates. Infectious complications are reasons to decline grafts due fear of post-transplant primary graft dysfunction. Mesenchymal stem cells a promising therapy currently investigated in treating injury. Full-term amniotic fluid-derived lung-specific mesenchymal cell treatment may regenerate damaged lungs. These have previously demonstrated inflammatory mediation other respiratory diseases, and we hypothesized that would improve...
In recent years, the field of graft preservation has made considerable strides in improving outcomes related to solid organ restoration and regeneration. Ex vivo lung perfusion (EVLP) line with devices treatments yielded promising results within preclinical clinical studies, potential improve quality. Its main benefit is render marginal declined donor lungs suitable for transplantation, ultimately increasing pool available transplantation. addition, using such therapies machine could also...
In severe acute respiratory distress syndrome (ARDS), extracorporeal membrane oxygenation (ECMO) is a life-prolonging treatment, especially among COVID-19 patients. Evaluation of lung injury progression challenging with current techniques. Diagnostic imaging or invasive diagnostics are risky given the difficulties intra-hospital transportation, contraindication biopsies, and potential for spread infections, such as in We have recently shown that particle flow rate (PFR) from exhaled breath...
Mechanical ventilation can be monitored by analysing particles in exhaled air as measured particle flow rate (PFR). This could a potential method of detecting ventilator-induced lung injury (VILI) before changes conventional parameters detected. The aim this study was to investigate PFR during different modes patients without pathology.A prospective conducted on mechanical the cardiothoracic intensive care unit (ICU). A PExA 2.0 device connected expiratory limb ventilator for continuous...
Lung transplantion (LTx) recipients have low long-term survival and a high incidence of bronchiolitis obliterans syndrome (BOS), an inflammation the small airways in chronic rejection lung allograft. There is great clinical need for minimally invasive biomarker BOS. Here, 644 different proteins were analyzed to detect biomarkers that distinguish BOS grade 0 from grades 1-3. The plasma 46 double transplant patients was using high-component, multiplex immunoassay enables analysis protein...
Primary graft dysfunction (PGD) remains a challenge for lung transplantation (LTx) recipients as leading cause of poor early outcomes. New methods are needed more detailed monitoring and understanding the pathophysiology PGD. The measurement particle flow rate (PFR) in exhaled breath is novel tool to monitor understand disease at proteomic level. In total, 22 recipient pigs underwent orthotopic left LTx were evaluated PGD on postoperative day 3. Exhaled particles (EBPs) by mass spectrometry...