A5052107377- Tissue Engineering and Regenerative Medicine
- Nanoparticle-Based Drug Delivery
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Neonatal Respiratory Health Research
- Respiratory Support and Mechanisms
- Peptidase Inhibition and Analysis
- Nanoplatforms for cancer theranostics
- Advanced X-ray Imaging Techniques
- Tracheal and airway disorders
- Inhalation and Respiratory Drug Delivery
- Diverse Scientific Research Studies
- Protease and Inhibitor Mechanisms
- Lung Cancer Research Studies
- Mesenchymal stem cell research
- Metal-Organic Frameworks: Synthesis and Applications
- Pulmonary Hypertension Research and Treatments
- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Cancer Cells and Metastasis
- Mechanical Circulatory Support Devices
- Healthcare and Environmental Waste Management
- RNA Interference and Gene Delivery
- Water Quality and Pollution Assessment
- Extracellular vesicles in disease
Lund University
2018-2023
Skåne University Hospital
2023
Alligator Bioscience (Sweden)
2022
Medicon Village
2022
Wallenberg Wood Science Center
2020-2021
Helmholtz Zentrum München
2014-2020
Ludwig-Maximilians-Universität München
2013-2020
German Center for Lung Research
2013-2020
Center for Molecular Medicine and Immunology
2020
Bioengineering Center
2019
Abstract Recent advances in 3D bioprinting allow for generating intricate structures with dimensions relevant human tissue, but suitable bioinks producing translationally tissue complex geometries remain unidentified. Here, a tissue‐specific hybrid bioink is described, composed of natural polymer, alginate, reinforced extracellular matrix derived from decellularized (rECM). rECM has rheological and gelation properties beneficial while retaining biologically inductive supporting maturation ex...
Metal-organic frameworks (MOFs) are promising platforms for the synthesis of nanoparticles diverse medical applications. Their fundamental design principles allow significant control framework architecture and pore chemistry, enabling directed functionalization nanomedical However, before applying novel nanomaterials to patients, it is imperative understand their potential health risks. In this study, nanosafety different MOF analyzed comprehensively The authors first evaluate effects MOFs...
Nanoparticles allow for controlled and targeted drug delivery to diseased tissues therefore bypass systemic side effects. Spatiotemporal control of release can be achieved by nanocarriers that respond elevated levels disease-specific enzymes. For example, matrix metalloproteinase 9 (MMP9) is overexpressed in tumors, known enhance the metastatic potency malignant cells, has been associated with poor prognosis lung cancer. Here, we report synthesis mesoporous silica nanoparticles (MSNs)...
Fibrotic disorders account for over one third of mortalities worldwide. Despite great efforts to study the cellular and molecular processes underlying fibrosis, there are currently few effective therapies. Dual-stage polymerization reactions an innovative tool recreating heterogeneous increases in extracellular matrix (ECM) modulus, a hallmark fibrotic diseases vivo. Here, we present clickable decellularized ECM (dECM) crosslinker incorporated into dynamically responsive poly(ethylene...
Accounting for about 1.5 million deaths annually, lung cancer is the prevailing cause of worldwide, mostly associated with long-term smoking effects. Numerous small-animal studies are performed currently in order to better understand pathogenesis disease and develop treatment strategies. Within this letter, we propose exploit X-ray dark-field imaging as a novel diagnostic tool detection on projection radiographs. Here, demonstrate living mice bearing tumors, that radiography provides...
Mesoporous silica nanoparticles (MSNs) exhibit unique drug delivery properties and are thus considered as promising candidates for next generation nano-medicines. In particular, inhalation into the lungs represents a direct, non-invasive route treating lung disease. To assess MSN biocompatibility in lung, we investigated bioresponse of avidin-coated MSNs (MSN-AVI), well aminated (uncoated) MSNs, after direct application mice. We quantified distribution, clearance rate, cell-specific uptake,...
Background Indications with poor T-cell infiltration or deficiencies in priming and associated unresponsiveness to established immunotherapies represent an unmet medical need oncology. CD40-targeting therapies designed enhance antigen presentation, generate new tumor-specific T cells, activate tumor-infiltrating myeloid cells remodel the tumor microenvironment, a promising opportunity meet this need. In study, we present first vivo data supporting role for tumor-associated (TAA)-mediated...
Non-invasive magnetic resonance imaging (MRI) is gaining significant attention in the field of tissue engineering, since it can provide valuable information on vitro production parameters and vivo performance. It e.g. be used to monitor morphology, location function regenerated tissue, integrity, remodeling resorption scaffold, fate implanted cells. Since cells are not visible using conventional MR techniques, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles routinely employed...
Abstract Herein is described the switchable fluorescence response of poly(methyl methacrylate) (PMMA) brushes. Chain end fluorescein labeled PMMA brushes are prepared by combining surface‐initiated atom transfer radical polymerization (SI‐ATRP) with a copper‐catalyzed alkyne‐azide cycloaddition (CuAAC) click reaction. Successful attachment confirmed measuring as‐prepared films. Utilizing co‐solvency in isopropanol‐water mixtures, responsive behavior end‐functionalized demonstrated changes...
Acute respiratory distress syndrome (ARDS) is a common cause of death in the intensive care unit, with mortality rates ~30–40%. To reduce invasive diagnostics such as bronchoalveolar lavage and time-consuming in-hospital transports for imaging diagnostics, we hypothesized that particle flow rate (PFR) pattern from airways could be an early detection method contribute to improving optimizing personalized therapies. Porcine models were ventilated mechanically. Lipopolysaccharide (LPS) was...
Acute respiratory distress syndrome (ARDS) is a life-threatening, high mortality pulmonary condition characterized by acute lung injury (ALI) resulting in diffuse alveolar damage. Despite progress regarding the understanding of ARDS pathophysiology, there are presently no effective pharmacotherapies. Due to complexity and multiorgan involvement typically associated with ARDS, animal models remain most commonly used research tool for investigating potential new therapies. Experimental...
Engineered nanomaterials (ENMs) are increasingly produced and used today, but health risks due to their occupational airborne exposure incompletely understood. Traditionally, nanoparticle (NP) toxicity is tested by introducing NPs cells through suspension in the growth media, this does not mimic respiratory exposures. Different methods introduce aerosolized cultured at air-liquid-interface (ALI) have been developed, require specialized equipment associated with higher cost time. Therefore,...
Abstract Mechanical ventilation is often required in patients with pulmonary disease to maintain adequate gas exchange. Despite improved knowledge regarding the risks of over ventilating lung, ventilator induced lung injury (VILI) remains a major clinical problem due inhomogeneities within diseased itself as well need increase pressure or volume oxygen life-saving measure. VILI characterized by increased physical forces exerted which results cell death, inflammation and long-term fibrotic...
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Nanoparticle-based targeted drug delivery holds promise for treatment of cancers. However, most approaches fail to be translated into clinical success due ineffective tumor targeting in vivo. Here, the potential mesoporous silica nanoparticles (MSN) functionalized with ligands EGFR and CCR2 is explored lung tumors. The addition active on MSNs enhances their uptake vitro but fails promote specific tumors vivo, when administered systemically via blood or locally immunocompetent murine cancer...
In severe acute respiratory distress syndrome (ARDS), extracorporeal membrane oxygenation (ECMO) is a life-prolonging treatment, especially among COVID-19 patients. Evaluation of lung injury progression challenging with current techniques. Diagnostic imaging or invasive diagnostics are risky given the difficulties intra-hospital transportation, contraindication biopsies, and potential for spread infections, such as in We have recently shown that particle flow rate (PFR) from exhaled breath...
<b>Background and aims:</b> The majority of current organoids are grown within Matrigel, a protein mixture secreted by mouse sarcoma cells, due to the fact that it mimics basement membrane contains growth factors support organoid formation. However, concerns have arisen as suitability Matrigel for studying normal regeneration stem cell behavior its cancerous origin. Hydrogels generated from pepsin extracted, decellularized extracellular matrix (dECM) solutions recently emerged potential...
Purpose: The purpose of the present study was to evaluate if x-ray dark-field imaging can help visualize lung cancer in mice. Materials and Methods: experiments were performed using mutant mice with high-grade adenocarcinomas. Eight animals pulmonary carcinoma eight control imaged radiography mode a prototype small-animal scanner three cancerous ones additionally CT mode. After imaging, lungs harvested for histological analysis. To determine their diagnostic value, conventional attenuation...
<b>Background:</b> Lung transplantation is the only curative approach for end stage lung disease, but tissue shortage remains a problem. Generating ex vivo could overcome shortages. Both synthetic and biologic scaffolds have been explored, yet both limitations. Biologic low reproducibility while lack biologically inductive properties. Hybrid materials, comprised of both, used engineering, limited to simple shapes. Recent advances in 3D printing may permit complex shapes, potential bioinks...
Abstract Mesenchymal stem cells (MSCs) have been studied for their potential benefits in treating acute respiratory distress syndrome (ARDS) and reported mild effects when trialed within human clinical trials. MSCs investigated preclinical models with efficacy administered at the time of lung injury. Human integrin α10β1-selected adipose tissue-derived (integrin α10β1-MSCs) shown immunomodulatory regenerative various disease models. We hypothesized that α10β1 selected-MSCs can be used to...
<b>Background:</b> The concept of transplantable, bioengineered lungs has been proposed as a solution to meet growing transplantation needs. One the ways potentially build complex lung structure is 3D bioprinting. Decellularized extracellular matrix (dECM) based materials are considered potential novel source material for bioinks because they have shown in other contexts provide suitable microenvironment regeneration. We hypothesized that using dECM porcine bioink would recapitulate...