A5044512795- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Effects of Radiation Exposure
- Nanoparticles: synthesis and applications
- Air Quality and Health Impacts
- Radiation Therapy and Dosimetry
- Energy and Environment Impacts
- Graphene and Nanomaterials Applications
- Cancer-related Molecular Pathways
- Synthesis and biological activity
- Metal complexes synthesis and properties
- Bacterial biofilms and quorum sensing
- Anesthesia and Neurotoxicity Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Plant Genetic and Mutation Studies
- Chemotherapy-induced organ toxicity mitigation
- Microfluidic and Bio-sensing Technologies
- Poxvirus research and outbreaks
- Polysaccharides and Plant Cell Walls
- bioluminescence and chemiluminescence research
- Cancer therapeutics and mechanisms
- Veterinary medicine and infectious diseases
- Cell Image Analysis Techniques
- RNA Interference and Gene Delivery
- Seaweed-derived Bioactive Compounds
Jan Kochanowski University
2016-2025
Instytut Chemii i Techniki Jądrowej
2010-2024
Instytut Medycyny Wsi im. Witolda Chodźki
2024
National Institute of Public Health
2024
Stockholm University
2010-2023
Kielce University of Technology
2022
Saponins are detergent-like substances showing antibacterial as well anticancer potential. In this study, the effects of saponins from Quillaja saponaria were analyzed against prokaryotic and eukaryotic cells. Multidrug-resistant clinical E. coli strains isolated human urine. As cells, CHO-K1 cell lines applied. Antibacterial effect ampicillin, streptomycin, ciprofloxacin in presence was measured by cultivation methods. Properties cells MTT test, hemolysis assay flow cytometry. Saponin has a...
Silica nanoparticles have an interesting potential in drug delivery, gene therapy and molecular imaging due to the possibility of tailoring their surface reactivity that can be obtained by modification. Despite these benefits, there is concern exposure humans certain types silica nanomaterials may lead significant adverse health effects. The motivation this study was determine kinetics cellular binding/uptake vinyl- aminopropyl/vinyl-modified into peripheral blood lymphocytes vitro, explore...
The physicochemical properties of metal complexes determine their potential applications as antitumor agents. In this study, the mononuclear cobalt(II) and copper(II) coordination compounds (stoichiometry: [Co(iaa)2(H2O)2]·H2O (iaa = imidazole-4-acetate anion), [Co(1-allim)6](NO3)2 (1-allim 1-allylimidazole), [Cu(iaa)2H2O] [Cu(1-allim)4(NO3)2]) ligands have been evaluated on human lung carcinoma A549 cells normal bronchial BEAS-2B cells. Designing chemical structure new agents possible...
Biodiesels represent more carbon-neutral fuels and are introduced at an increasing extent to reduce emission of greenhouse gases. However, the potential impact different types blend concentrations biodiesel on toxicity diesel engine emissions still relatively scarce some contradictory. The objective present work was compare exhaust particles (DEP) from combustion two 1st-generation fuels: 7% fatty acid methyl esters (FAME; B7) 20% FAME (B20) a 2nd-generation FAME/HVO (synthetic hydrocarbon...
Epidemiological data indicate that exposure to diesel exhaust particles (DEPs) from traffic emissions is associated with higher risk of morbidity and mortality related cardiovascular pulmonary diseases, accelerated progression atherosclerotic plaques, possible lung cancer. While the impact DEPs combustion fossil fuel on human health has been extensively studied, current knowledge biofuels provides limited inconsistent information about its mutagenicity genotoxicity, as well adverse risks....
Abstract We studied the effects of Aeroxide P25 titanium dioxide nanoparticles (TiO 2 NPs) with a diameter 21 nm on induction DNA damage and long-term survival three human cell lines: hepatocellular liver carcinoma HepG2, colorectal adenocarcinoma HT29 lung A549. The endpoints examined were breakage estimated by comet assay oxidative base recognized formamide-pyrimidine glycosylase (FPG) FPG + assay, frequencies histone γH2AX foci micronuclei, apoptosis, metabolic activity measured...
Abstract Candidate ionising radiation exposure biomarkers must be validated in humans exposed vivo. Blood from patients undergoing positron emission tomography–computed tomography scan (PET-CT) and skeletal scintigraphy (scintigraphy) was drawn before (0 h) after (2 the procedure for correlation analyses of response selected with dose other available patient information. FDXR , CDKN1A BBC3 GADD45A XPC, MDM2 expression determined by qRT-PCR, DNA damage (γH2AX) flow cytometry, reactive oxygen...
Hypothermia during in vitro irradiation of human peripheral blood lymphocytes (PBL) affects the level chromosome aberrations. The molecular mechanisms this phenomenon are not fully understood. aim our study was to examine effect hypothermia on dose-response relationship for dicentric chromosomes and γ-H2AX (phosphorylated histone H2AX) foci. In addition, inter- intra-individual variability assessed relation temperature.PBL were kept at 0.8, 20 37°C then exposed gamma-rays (from 0-3 Gy)....
Low temperature (hypothermia) during irradiation leads to a reduced frequency of micronuclei in TK6 cells and it has been suggested that perturbation cell cycle progression is responsible for this effect. The aim the study was test hypothesis.Human lymphoblastoid were treated by combination hypothermia (0.8°C) ionizing radiation varying order (hypothermia before, or after irradiation) scored. Growth assay two-dimensional flow cytometry used analyze kinetics following irradiated at 0.8°C...
Purpose: Low temperature at exposure has been shown to act in a radioprotective manner the level of cytogenetic damage. It was suggested be due an effective transformation DNA damage chromosomal low temperature. The purpose study analyze kinetics aberration formation during first hours after exposing human peripheral blood lymphocytes ionizing radiation 0.8 °C and 37 °C.Materials methods: To this end, we applied technique premature chromosome condensation. In addition, response analyzed by...
Biodosimetric methods used to measure the effects of radiation are critical for estimating health risks irradiated individuals or populations. The direct measurement radiation-induced γ-H2AX foci in peripheral blood lymphocytes is one approach that provides a useful end point triage. Despite documented advantages assay, there considerable variation among laboratories regarding formation same exposure conditions and cell lines. Taking this into account, goal our study was evaluate influence...
Coralyne is a synthetic analog of berberine related to protoberberine-isoquinoline alkaloids. Isoquinoline derivatives and analogs are renowned as potent radiosensitizers with potential medical application. In the present study, we investigated effect coralyne on cell death, cytoskeletal changes cycle progression irradiated A549 cells. A clonogenic assay revealed that pretreatment decreased viability cells in time- dose-dependent manner. Moreover, exposure ionizing radiation (IR) markedly...
Pseudomonas aeruginosa infection is problematic in patients with cystic fibrosis (CF). P. secretes a diversity of pigments, such as pyocyanin and pyoverdine. The aim this study was to evaluate the effects complexes nickel(II) ([Ni(iaa)2(H2O)2]·H2O (iaa = imidazole-4-acetate anion), [Ni(1-allim)6](NO3)2 (1-allim 1-allylimidazole) NiCl2 on pyoverdine production by 23 strains isolated from under growth conditions specific for CF respiratory system. antibacterial biophysical properties tested...
Gynaecologic cancers are common among women and treatment includes surgery, radiotherapy or chemotherapy, where the last two methods induce DNA damage in non-targeted cells like peripheral blood lymphocytes (PBL). Damaged normal can transform leading to second malignant neoplasms (SMN) but level of risk impact modifiers is not well defined. We investigated how alone combination with chemotherapy PBL cervix endometrial cancer patients during therapy. Blood samples were collected from nine...
Abstract Candidate ionising radiation exposure biomarkers must be validated in humans exposed vivo . Blood from patients undergoing positron emission tomography - computed scan (PET-CT) and skeletal scintigraphy (scintigraphy) was drawn before (0 h) after (2 the procedure for correlation analyses of response selected with dose other available patient information. FDXR , CDKN1A BBC3 GADD45A XPC MDM2 expression determined by qRT-PCR, DNA damage (γH2AX) flow cytometry, reactive oxygen species...
In this work we present a phase-retrieval-based approach to quantifying the diffusion of drugs through biomembranes or biofilms. So far, phenomenon was studied based on fringe orientation resulting from refractive index gradient in vicinity interface. The result is usually obtained with Mach-Zehnder interferometer an imaging system. This limits spatial resolution method and does not allow observation local changes diffusion. For reason, propose use single-shot phase retrieval by utilizing...