A5100404875- Ubiquitin and proteasome pathways
- Plant biochemistry and biosynthesis
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Coccidia and coccidiosis research
- Parasitic Infections and Diagnostics
- Traditional Chinese Medicine Analysis
- Endoplasmic Reticulum Stress and Disease
- Animal Virus Infections Studies
- Cholesterol and Lipid Metabolism
- Mitochondrial Function and Pathology
- Metal and Thin Film Mechanics
- Plant-Microbe Interactions and Immunity
- RNA modifications and cancer
- Helminth infection and control
- Microbial Community Ecology and Physiology
- ATP Synthase and ATPases Research
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Peptidase Inhibition and Analysis
- Autophagy in Disease and Therapy
China Agricultural University
2014-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2022
Stowers Institute for Medical Research
2020
Shenyang Agricultural University
2020
In Arabidopsis (Arabidopsis thaliana) plants growing under normal conditions, DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN2A (DREB2A) is present at low levels because it ubiquitinated and destabilized by DREB2A INTERACTING PROTEIN1 (DRIP1) DRIP2 through 26S proteasome-mediated proteolysis. Drought stress counteracts the ubiquitination proteolysis of DREB2A, thus allowing accumulation sufficient amounts protein to activate downstream gene expression. The mechanisms leading drought...
The apicomplexan parasites Eimeria spp. are the causative agents of coccidiosis, a disease with significant global impact on poultry industry. complex life cycle involves exogenous (sporogony) and endogenous (schizogony gametogony) stages. Unfortunately, genetic regulation these highly dynamic processes, particularly for genes involved in specific developmental phases, is not well understood.
Protein ubiquitination is a major post-translational modification important for diverse biological processes. In wheat (Triticum aestivum) and Arabidopsis thaliana, STRESS-ASSOCIATED PROTEIN 5 (SAP5) involved in drought tolerance, acting as an E3 ubiquitin ligase to target DRIP MBP-1 degradation. To identify further proteins of SAP5, we implemented two independent approaches this work. We used ubiquitylome capture with di-Gly-Lys antibody-based peptide enrichment affinity purification...
Coccidiosis, a parasitic disease caused by single or multiple Eimeria species, leads to significant economic losses in the poultry industry. The life cycle includes schizogony, gametogony, and sporogony. To investigate dynamics of gene expression regulatory networks during development acervulina, we employed time-course transcriptomics rigorously compare patterns between precocious line (PL) wild type (WT) E. acervulina. results revealed that PL enters into gametogony 12 h earlier than WT,...
The glycoprotein 5 (GP5) of porcine reproductive and respiratory syndrome virus (PRRSV) is a multi-functional protein that plays important roles in assembly, entry viral anti-host responses. In the present study, we investigated cellular binding partners GP5 by using lentivirus transduction coupled with immunoprecipitation mass spectrometry. There were about 40 proteins identified high Confidence Icons MS/MS. Ingenuity Pathway Analysis (IPA) indicated these could be assigned to different...
ABSTRACT SETD2 is the sole methyltransferase that tri-methylates histone H3 at lysine 36 in mammals. It has an extended N-terminal region which absent its yeast homolog Set2. The function of this poorly characterized regulating stability been reported. However, how regulates half-life and consequences cellular accumulation unclear. Here we show contains disordered regions targeted for degradation by proteasome. marked increase global H3K36me3 occurs on removal segment results a non-canonical...
LINE-1 (long interspersed element 1) is the only currently known active autonomous transposon in humans, and its retrotransposition may cause deleterious effects on structure function of host cell genomes result sporadic genetic diseases. Host cells therefore developed defence strategies to restrict mobilization. In this study, we demonstrated that IFN-inducible Schlafen5 (SLFN5) potently inhibits retrotransposition. Mechanistic studies revealed SLFN5 interrupt ribonucleoprotein particle...