A5050142997- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Silk-based biomaterials and applications
- Postharvest Quality and Shelf Life Management
- Phytochemicals and Antioxidant Activities
- Plant Stress Responses and Tolerance
- Hepatocellular Carcinoma Treatment and Prognosis
- Plant Reproductive Biology
- Plant Physiology and Cultivation Studies
- Light effects on plants
- Horticultural and Viticultural Research
- Pancreatic and Hepatic Oncology Research
- Insect Resistance and Genetics
- Plant tissue culture and regeneration
- Silkworms and Sericulture Research
- Gallbladder and Bile Duct Disorders
- Circadian rhythm and melatonin
- Esophageal Cancer Research and Treatment
- Inflammatory Biomarkers in Disease Prognosis
- TGF-β signaling in diseases
- Plant responses to water stress
- Antioxidant Activity and Oxidative Stress
- MRI in cancer diagnosis
- RNA Research and Splicing
Sun Yat-sen University
2023-2024
The First Affiliated Hospital, Sun Yat-sen University
2023-2024
China Agricultural University
2020-2023
Southwest University
2017-2023
Ministry of Agriculture and Rural Affairs
2022-2023
Lanzhou University
2022
First Hospital of Lanzhou University
2022
Fujian Agriculture and Forestry University
2018-2021
State Key Laboratory of Silkworm Genomic Biology
2017-2019
Abstract Anthocyanin production in bicolored sweet cherry (Prunus avium cv. Rainier) fruit is induced by light exposure, leading to red coloration. The phytohormone abscisic acid (ABA) essential for this process, but the regulatory relationships that link and ABA with anthocyanin-associated coloration are currently unclear. In study, we determined treatment of increased anthocyanin accumulation participates light-modulated cherry. Two B-box (BBX) genes, PavBBX6/9, were highly treatments, as...
Drought stress substantially reduces the productivity of apple plants and severely restricts development industry. Malus sieversii, wild apples with excellent drought resistance, is a valuable resource for rootstock improvement cultivated (Malus domestica). miRNAs their targets play essential roles in plant growth responses, but responses are unknown. Here, we demonstrate that microRNA156ab upregulated M. sieversii response to stress. Overexpressing msi-miR156ab promoted auxin accumulation,...
In this study, the fruit quality, anthocyanin content and antioxidant enzyme activities of skin pulp southern blueberries (Vaccinium corymbosum) from China y at five developmental stages (green, pink, red, purple blue) were investigated anthocyanins characterized quantified by HPLC during considered stages. The results indicatated that contents phenolic, flavonoids as well varied depending on correlation values between total phenolic (TPC), (TFC) (TAC) significant. highest activity...
Acid rain is a serious worldwide environmental problem which reduces the growth and yield of crops. Melatonin, as pleiotropic molecule has been known to improve stress tolerance by limiting oxidative damage plants exposed adverse environments. However, role exogenous melatonin particularly on antioxidant compounds in tomato fruits under abiotic condition remains inexpressible. This observation aims identify influence treatment simulated acid (SAR) fruit qualities, phenolics, flavonoids,...
Drought stress severely restricts crop yield and quality. Small noncoding RNAs play critical roles in plant growth, development, responses by regulating target gene expression, but their drought tolerance apple (Malus domestica) are poorly understood. Here, we identified various small targets from the wild species Malus sieversii via high-throughput sequencing degradome analysis. Forty known microRNAs (miRNAs) eight new were differentially expressed response to 2 or 4 h of treatment. We...
Fruit firmness is an important economical trait in sweet cherry (Prunus avium L.) where the change of this related to cell wall degradation. Xyloglucan endotransglycosylase/hydrolase (XTH) and polygalacturonases (PGs) are critical cell-wall-modifying enzymes that occupy a crucial position fruit ripening softening. Herein, we identified 18 XTHs 45 PGs designated PavXTH1-18 PavPG1-45 based on their locations genome cherry. We provided systematical overview PavXTHs PavPGs, including...
Softening is a key step during fruit ripening that modulated by the interplay between multiple phytohormones. The antagonistic action of abscisic acid (ABA) and auxin determines rate softening. However, transcription factors integrate ABA signals to regulate softening remain be determined. In this study, we identified several DNA-binding with One Finger (Dof) essential for ABA-promoted softening, based on transcriptome analysis two sweet cherry (Prunus avium L.) varieties different firmness....
Under drought stress, reactive oxygen species (ROS) overaccumulate as a secondary stress that impairs plant performance and thus severely reduces crop yields. The mitigation of ROS levels under is therefore crucial for tolerance. MicroRNAs (miRNAs) are critical regulators development responses. However, the complex molecular regulatory mechanism by which they function during especially in drought-triggered scavenging, not fully understood. Here, we report newly identified drought-responsive...
Plant-specific SQUAMOSA promoter-binding protein-like (SPL) transcription factors play important regulatory roles during plant growth and development, fruit ripening, inflorescence branching, biotic abiotic stresses. However, there have been no identification or systematic studies of the SPL gene family in sweet cherry. In this study, 12
B-BOX proteins are zinc finger transcription factors that play important roles in plant growth, development, and abiotic stress responses. In this study, we identified 15 PavBBX genes the genome database of sweet cherry. We systematically analyzed gene structures, clustering characteristics, expression patterns these during fruit development response to light various hormones. The were divided into five subgroups. promoter regions contain cis-acting elements related hormones, stress. qRT-PCR...
Basic helix-loop-helix proteins (bHLHs) play very important roles in the anthocyanin biosynthesis of many plant species. However, reports on blueberry biosynthesis-related bHLHs were limited. In this study, six identified from genome data through homologous protein sequence alignment. Among these bHLHs, VcAN1, VcbHLH42-1, VcbHLH42-2 and VcbHLH42-3 clustered into one group, while VcbHLH1-1 VcbHLH1-2 other group. All bHLH-MYC_N domain, had DNA binding sites reported conserved amino acids bHLH...
Purpose To investigate the value of three-dimensional ultrasound fusion imaging (3DUS FI) technique for guiding needle placement in hepatocellular carcinoma (HCC) thermal ablation.
Abstract Nucleosome-depleted open chromatin regions (OCRs) often harbor transcription factor (TF) binding sites that are associated with active DNA regulatory elements. To investigate the regulation of silk-protein genes, molecules isolated from silk glands third-day fifth-instar silkworm larvae and embryo-derived ( BmE ) cells were subjected to formal dehyde-assisted isolation elements (FAIRE) high-throughput sequencing. In total, 68,000 OCRs identified, a number TF-binding motifs...
Sericin is a natural protein with high application potential, but the research on its efficacy very limited. In this study, anti-inflammatory mechanism of sericin was investigated. Firstly, composition extracts determined by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). This then combined Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), it confirmed that anti-inflammation ability positively correlated purity 1 protein. Finally, RNA-seq...
Brunfelsia acuminata is a popular ornamental plant with different colors resulted from the rapid change of color after blooming. The petals at day one (purple), three (white and purple) five (white) were used to analyze reason flower by comparative proteomics approach, gas chromatography coupled time-of-flight mass analyzer (GC-TOF-MS) quantitative real-time PCR (qRT-PCR). results showed that 52 identified proteins classified into eight functional groups, 6% which related anthocyanin...
Calcium depletion is a valuable non-invasive tool for studying skeletal system disorders. A low calcium diet (LCD) was used to examine the pathophysiological characteristics and molecular mechanisms of osteoporotic damage in caged laying hens. Sixty 64-wk-old hens were randomly housed single-bird cages, cages divided into 2 treatments: fed regular (RCD, 3.69%) or LCD (1.56%) 8 wk. The diet-induced changes serum bone remodeling indicators, strength, microstructure distal femur, gene...
Okra (Abelmoschus esculentus L.) is a difficult species to regenerate in vitro because of its recalcitrant nature. Whitish colored 'non-embryogenic' callus formation major problem okra tissue culture. In this study, different levels inorganic–nitrogen (KNO3 and NH4NO3 with 1:2 ratios, 30–60 mM) MS media PGRs combinations were tested obtain friable embryogenic masses. Hypocotyls produced the highest induction (71%) on containing 1.0 mgL−1 BAP + 1.5 2, 4-D, whereas cotyledons induced (58%)...
Drought is the main environmental factor that limits yield and quality of apples (Malus × domestica) grown in arid semi-arid regions. Nuclear Ys (NF-Ys) are important transcription factors involved regulation plant growth, development, various stress responses. However, function NF-Y genes poorly understood apples. Here, we identified 43 genome conducted an initial functional characterization apple NF-Y. Expression analysis members M. sieversii revealed a large number NF-Ys were highly...
Immune checkpoint inhibitors (ICIs) have been increasingly used to treat hepatocellular carcinoma (HCC). Prognostic biomarkers are an unmet need. We aimed develop a prognostic nomogram for patients with unresectable HCC receiving ICIs therapy. A total of 120 treatment were enrolled in this study. Patients randomly divided into training set (n = 84) and validation 36) 7:3 ratio. Clinical characteristics retrospectively analyzed. Serum α-fetoprotein protein (AFP) response was defined as...