This study examined effect of a dietary synbiotic supplement on the concentrations plasma thyroid hormones, expressions heat shock protein 70 (HSP70), and intestinal histomorphology in broiler chickens exposed to cyclic stress (HS). Three hundred sixty day old male Ross 708 chicks were randomly distributed among 3 treatments containing (PoultryStar meUS) at 0 (control), 0.5 (0.5×), 1.0 (1.0×) g/kg. Each treatment contained 8 replicates 15 birds each housed floor pens temperature lighting...

Bacillus subtilis is one of the most popular commercial probiotics used in farm animal production. However, its potential mechanisms are not very clear. The aim this study was to investigate effects dietary on intestinal histomorphology, innate immunity, microbiota composition, transcriptomics, and related metabolomics. Twenty-four 48-week-old Lohman Pink-shell laying hens were randomly divided into two groups: a basic diet supplemented with (0.5 g/kg) for 9-week experiment. At end...

The aim of this study was to examine the effect a dietary synbiotic supplement on cecal microflora, antioxidant status, and immune response broiler chickens under heat stress (HS). A total 360 one-day-old male Ross 708 chicks were randomly distributed among 3 treatments containing (PoultryStar consists Bifidobacterium animalis, Enterococcus faecium, Lactobacillus reuteri, Pediococcus acidilactici, fructooligosaccharides) at 0 (control), 0.5 (0.5X), 1.0 (1.0X) g/kg. Each treatment contained 8...

Studies have demonstrated that obesity and osteoporosis are linked disorders in humans. This study examined the hypothesis excessive lipid consumption affects bone metabolism laying hens. A total of one hundred 63-wk-old hens were randomly divided into 2 treatments fed either a regular layer diet (control) or high energy low protein (HE-LP; experimental treatment) for 80 d. Egg production, feed intake, BW recorded at various days during treatment. At d 80, ten chosen birds per treatment...

The aim of this study was to examine effects various daylight exposure during the 24-h light-dark (L-D) cycle on growth performance, skeletal health, and welfare state in broilers. Environmental photoperiod related circadian clock, L-D cycle, are important factors maintaining productive pathophysiological homeostasis, psychological reaction humans animals. Currently, lighting programs as management tools for providing a satisfactory environmental condition have been used commercial broiler...

The aim of this study was to investigate the effects osteocalcin (OCN) on fatty liver hemorrhagic syndrome (FLHS) in aged laying hens. Thirty 68-week-old White Plymouth hens were randomly assigned into conventional single-bird cages, and cages allocated one 3 treatments (n = 10): normal diet (ND + vehicle, ND V), high-fat (HFD HFD OCN (3 μg/bird, 1 time/2 d, i.m.) for 40 d. At day 30, oral glucose tolerance tests (OGTT) insulin (ITT) performed. end experiment, euthanized followed by blood...

Osteoporosis is a common degenerative metabolic bone disease in caged laying hens. Intensive egg production mobilizing large amounts of Ca from for eggshell formation, consequently leading to deficiency, has been recognized as critical factor causing osteoporosis commercial The aim this study was examine the effect deficiency on function gut microbiota-bone axis and related traits health Twenty-four 48-week-old hens were fed control diet (Control, 3.72%) or low (LC, 2.04%) 60 d (n = 12)....

The objective of the study was to investigate differences in eggshell quality, bone quality and serum biochemistry markers associated with changes age dietary soybean oil levels laying hens. A total 54, 19-week-old Hy-Line Brown hens were housed 18 battery cages (3 birds/cage) randomly divided into three diet treatments for 90 d: control-fat (CF, 1.9% oil), moderate-fat (MF, 7% oil) high-fat (HF, 10% oil). hens' body weights (BW), egg production, weights, thickness femoral diameter higher at...

This study was to investigate the effects of Bacillus subtilis on production performance and bone pathophysiological characteristics layers. Twenty-four 48-week-old Lohmann Pink-shell laying hens were randomly divided into two groups: a basic diet (control) mixed with (0.5 g/kg) for 60-day trial. Statistically, independent-sample t-test used assess treatment differences. The results showed that supplementation improved percent marketable eggs (p < 0.05) reduced numbers broken soft-shelled...

Fatty liver is a common energy metabolic disorder in caged laying hens. Considering that the egg industry shifting from conventional cages to alternative housing systems such as enriched cages, objective of this study was determine effects perches on fat deposition and health Three hundred twenty-four 17-wk-old White Leghorn hens were housed 1 4 treatments with 9 per cage. Treatment never had access during their life cycle. 2 pullet phase only. 3 always perches. Liver weight, abdominal pad...

Abstract GA 3 and 4 (gibberellins) play an important role in controlling gravitropism tension wood formation woody angiosperms. In order to improve our understanding of the on xylem cell G‐layer, we studied effect uniconazole‐P, which is inhibitor biosynthesis, by gravity Fraxinus mandshurica Rupr. var. japonica Maxim. seedlings. Forty seedlings were divided into two groups; one group was placed upright other tilted. Each further four sub‐groups subjected following treatments: 3.43 × 10 −9...

Calcium depletion is a valuable non-invasive tool for studying skeletal system disorders. A low calcium diet (LCD) was used to examine the pathophysiological characteristics and molecular mechanisms of osteoporotic damage in caged laying hens. Sixty 64-wk-old hens were randomly housed single-bird cages, cages divided into 2 treatments: fed regular (RCD, 3.69%) or LCD (1.56%) 8 wk. The diet-induced changes serum bone remodeling indicators, strength, microstructure distal femur, gene...

Osteocalcin (OC) is a sensitive biochemical marker for evaluating bone turnover in mammals. The role of avian OC less clear because the need chicken assay. Our objectives were to develop an assay using indirect competitive ELISA detecting serum and use examine effects perches on remodeling caged hens. Anti-chicken polyclonal antibody was produced by immunization rabbits with recombinant from Escherichia coli. Chicken extracted used as coated protein, purified calibration. limit detection...

Osteocalcin (OCN) has a function in preventing fatty liver hemorrhagic syndrome (FLHS) poultry. The aim of this study was to investigate the effects OCN on fat emulsion stimulated chicken embryonic hepatocytes and related signaling pathways. primary were isolated from incubated 15-day (E15) pathogen free eggs cultured with dulbecco's modified eagle medium (DMEM). After hepatocyte density reached 80%, cells divided into 5 groups: control group (CONT), (FE, 10% FE, v/v), FE ucOCN at 1 ng/mL...

Fatty liver hemorrhage syndrome (FLHS) is the leading cause of noninfectious mortality in caged layers worldwide. Osteocalcin (OCN) a protein secreted by osteoblasts, and its undercarboxylated form (ucOCN) acts as multifunctional hormone that protects laying hens from FLHS. Lipophagy selective autophagy breaks down lipid droplets (LDs) through lysosomes, defective lipophagy associated with The aim this study was to investigate effects ucOCN on chicken embryonic hepatocytes function...