A5022220235- Infant Nutrition and Health
- Enzyme Production and Characterization
- Advanced Glycation End Products research
- Enzyme Structure and Function
- Pediatric Hepatobiliary Diseases and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Neonatal Respiratory Health Research
- Breastfeeding Practices and Influences
- Diabetes and associated disorders
- Gut microbiota and health
- Protein Hydrolysis and Bioactive Peptides
- Cytomegalovirus and herpesvirus research
- Food Quality and Safety Studies
- Epigenetics and DNA Methylation
- Probiotics and Fermented Foods
- Food composition and properties
- Garlic and Onion Studies
- Enterobacteriaceae and Cronobacter Research
- Biofuel production and bioconversion
- Cancer Research and Treatments
- Intracerebral and Subarachnoid Hemorrhage Research
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Chromatin Dynamics
- Pharmacological Effects of Natural Compounds
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Korea Advanced Nano Fab Center
2022
Seoul National University
2003-2018
Genexine (South Korea)
2009
Ulcerative colitis is an inflammatory bowel disease (IBD) with relapsing and remitting patterns, it caused by varied factors, such as the intestinal inflammation extent duration. We examined preventative effects of human milk oligosaccharides (HMOs) on epithelial barrier integrity in interleukin (IL)-6-induced cell model dextran sodium sulfate (DSS)-induced acute mouse model. HMOs including 2'-fucosyllactose (FL) 3-FL positive controls fructooligosaccharide (FOS) 5-acetylsalicylic acid...
Aims: Isomaltulose (palatinose) is a slowly digestible sucrose isomer that can reduce both the glycemic and insulinemic response to foods. The aim of this study was clone express isomerase (SIase) gene characterize protein responsible for production isomaltulose in micro-organism Enterobacter sp. FMB-1. Methods Results: A cosmid containing c. 6 kbp region encoding an SIase identified. 5969-bp chromosomal DNA fragment covering (esi) FMB-1 sequenced. Although contained several open reading...
This study was done to modify erythritol change its physicochemical and sensory properties. Erythritol, a four-carbon sugar alcohol, transglycosylated by Bacillus stearothermophilus maltogenic amylase with maltotriose as donor molecule. The presence of various transglycosylation products confirmed TLC high performance ion exchange chromatography (HPIC). major transfer product purified gel filtration on Bio-Gel P-2. Examination LC-MS, matrix-assisted laser desorption ionisation-time flight...
Cerebral ischemic stroke is one of the leading causes death and disability worldwide. 2′-fucosyllactose (2′-FL), a human milk oligosaccharide, exerts anti-inflammatory effects plays protective role in arterial thrombosis; however, its remains unclear. This study aimed to investigate neuroprotective 2′-FL potential mechanisms mouse model stroke. Neurological score behavior tests revealed that promoted recovery neurological deficits motor function middle cerebral artery occlusion (MCAO) mice,...
Abstract 2′-Fucosyllactose (2′-FL), a major component of fucosylated human milk oligosaccharides, is beneficial to health in various ways like prebiotic effect, protection from pathogens, anti-inflammatory activity and reduction the risk neurodegeneration. Here, whole-cell fluorescence biosensor for 2′-FL was developed. Escherichia coli ( E. ) engineered catalyse cleavage into l -fucose lactose by constitutively expressing α- -fucosidase. ∆L YA, which lacZ deleted lacY retained, employed...
Intracerebral hemorrhage (ICH) occurs when brain blood vessels rupture, causing inflammation and cell death. 2-Fucosyllactose (2FL), a human milk oligosaccharide, has potent antiapoptotic anti-inflammatory effects. The purpose of this study was to examine the protective effect 2FL in cellular rodent models ICH. Hemin added primary rat cortical neuronal BV2 microglia coculture simulate ICH vitro. IBA1 MAP2 immunoreactivities were used determine survival. significantly increased IBA1, while it...
The ingestion of supplemental human milk oligosaccharides (HMOs) is associated with a lower risk inflammatory diseases and infections. However, whether HMOs play an important role in regulating platelet aggregation thrombus formation remains unclear. current study aimed to investigate the antiplatelet antithrombotic effects HMOs. Among all HMOs, 2′-fucosyllactose (2′-FL) could significantly inhibit collagen-related peptide-induced aggregation, granule secretion, intracellular calcium...
Cerebral ischemic stroke is one of the leading causes death and disability worldwide. 2’-fucosyllactose (2’-FL), a human milk oligosaccharide, has anti-inflammatory effects plays protective role in arterial thrombosis, but its remains unclear. This study aimed to investigate neuroprotective 2’-FL potential mechanisms mouse model stroke. The administration led reduction size cerebral infarct reactive oxygen species (ROS)-related products brain MCAO mice. Further, promotes recovery...
Abstract The concentration of human milk oligosaccharides has gained significant attention recently due to their positive effects on infant health. However, the 3-fucosyllactose(3-FL) in Korean mother not been studied. Therefore, this study aimed analyze 3-FL using mass spectrometry. analytical method for quantitating was validated have high precision, accuracy, linearity, specificity, stability, recovery, and reproducibility. then determined 102 samples. results showed that increased during...