A5035973487- Protease and Inhibitor Mechanisms
- Blood Coagulation and Thrombosis Mechanisms
- Neurological Disease Mechanisms and Treatments
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Marine Sponges and Natural Products
- Echinoderm biology and ecology
- Additive Manufacturing and 3D Printing Technologies
- Proteoglycans and glycosaminoglycans research
- Seaweed-derived Bioactive Compounds
- Neurological Disorders and Treatments
- Biochemical effects in animals
- Inflammasome and immune disorders
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Biological and pharmacological studies of plants
- Planetary Science and Exploration
- Antimicrobial agents and applications
- Wound Healing and Treatments
- Traditional Chinese Medicine Analysis
- Inflammation biomarkers and pathways
- Cancer-related molecular mechanisms research
- HIV Research and Treatment
- Phytochemical Studies and Bioactivities
- Medicinal Plants and Neuroprotection
- Marine and coastal plant biology
- Phytochemistry and Bioactive Compounds
Chengdu Women's and Children's Central Hospital
2024
University of Electronic Science and Technology of China
2024
East China University of Science and Technology
2021-2024
Kunming Medical University
2018-2024
Dongguan University of Technology
2022-2024
Kunming Institute of Botany
2013-2019
Chinese Academy of Sciences
2013-2019
University of Chinese Academy of Sciences
2015-2019
Sun Yat-sen University
2013-2014
In order to search for sulfated polysaccharides in different invertebrate connective tissues and examine their biological activities, we have isolated three types of from the body wall sea cucumbers Holothuria edulis, Apostichopus japonicas nobilis. The physicochemical properties anticoagulant activities these were examined compared. chemical composition analysis nuclear magnetic resonance (NMR) indicate that two polysaccharides, fucan fucosylated chondroitin sulfate (FuCS), found all...
Significance Selective inhibition of the intrinsic coagulation pathway is a promising strategy for developing safer anticoagulants without serious bleeding consequences. We prepared and identified series oligosaccharides as inhibitors tenase, which final rate-limiting enzyme complex in an attractive but less explored target due to lack pure selective inhibitor. Analysis these purified reveals precise structure fucosylated glycosaminoglycan. Among oligosaccharides, nonasaccharide minimum...
Sulfated fucans, the complex polysaccharides, exhibit various biological activities. Herein, we purified two fucans from sea cucumbers Holothuria edulis and Ludwigothurea grisea. Their structures were verified by means of HPGPC, FT-IR, GC–MS NMR. As a result, novel structural motif for this type polymers is reported. The have unique structure composed central core regular (1→2) (1→3)-linked tetrasaccharide repeating units. Approximately 50% units L. grisea (100% H. fucan) contain sides...
Vascular dementia (VD) is the second most common syndrome worldwide, and effective treatments are lacking. Gastrodia elata Blume (GEB) has been used in traditional Chinese herbal medicine for centuries to treat cognitive impairment, ischemic stroke, epilepsy, dizziness. Gastrodin (p-hydroxymethylphenyl-b-D-glucopyranoside, Gas) Gastrodigenin (p-hydroxybenzyl alcohol, HBA) main bioactive components of GEB. This study explored effects Gas HBA on dysfunction VD their possible molecular...
Vascular dementia (VaD) is the second most common type of and has become a major public health challenge as global population ages. VaD caused by cerebrovascular disease, patients with have been reported to also Alzheimer’s pathologies, which formation neurofibrillary tangles amyloid plaques that are mainly composed hyperphosphorylated Tau β (Aβ) respectively. However, mechanisms not completely understood, very few drugs available treat this condition. Gastrodin (Gas) main bioactive...
Rapid hemostasis and antibacterial properties are essential for novel wound dressings to promote healing. In particular, timely rapid could be of benefit reduce the mortality caused by excessive bleeding loss. Herein, we present a strategy combining electrospinning technology with post-modification prepare multifunctional dressing, cellulose diacetate-based composite dressing (CDCE), activity. It is interesting that CDCE had superhydrophilicity, high water absorption, strong absorbing...
Abstract A native fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata (nHG), mainly branched with Fuc3S4S, exhibited potent anticoagulant activity by intrinsic tenase iXase (FIXa-FVIIIa complex) and antithrombin-dependent factor IIa (FIIa) inhibition, but also had the effects of FXII activation platelet aggregation. For screening a selective inhibitor, depolymerized nHG (dHG-1 ∼ –6) pure octasaccharide (oHG-8) were prepared. Like nHG, dHG-1 –6 oHG-8 could potently...
Acute exacerbation of chronic obstructive pulmonary disease (AECOPD) is associated with high mortality rates. Viral and bacterial coinfection the primary cause AECOPD. How these microbes influences host inflammatory response gut microbiota composition not entirely understood.We developed a mouse model AECOPD by cigarette smoke exposure sequential infection influenza H1N1 virus non-typeable Haemophilus influenzae (NTHi). titer was determined using MDCK cells chocolate agar plates,...
Abstract A nonasaccharide (FG9) derived from natural fucosylated glycosaminoglycan (FG) is identified as a selective intrinsic factor Xase complex (FIXa-FVIIIa-Ca2+-phospholipid, FXase) inhibitor that possesses potential inhibition of venous thrombus in rats and shows negligible bleeding risk. The mechanism molecular target the for FXase were systematically investigated compared with low weight heparin (LMWH). Our results showed FG9 dose-dependently inhibited FX activation by noncompetitive...
Chronic obstructive pulmonary disease (COPD), a major cause of morbidity and mortality worldwide, is widely considered to be related cigarette smoke (CS), viral infections trigger acute exacerbation COPD (AECOPD). Isoforskolin (ISOF) bioactive component from the plant Coleus forskohlii , native Yunnan in China. It has been demonstrated that ISOF anti-inflammatory effect on lung injury animal models. In present study, we investigated efficacy mechanism for prevention treatment AECOPD. Mice...
Background: Sepsis is initiated by the dysfunctional response of host immune system to infection. Septic shock and acute lung injury (ALI) are main etiology death caused sepsis. Glucocorticoids, which commonly used in clinic antagonize inflammatory sepsis, may cause serious side effects. Isoforskolin (ISOF) from plant Coleus forskohlii stimulates adenylyl cyclase, increases cAMP level inhibits response. The aim this study was investigate synergistic effect ISOF with dexamethasone (DEX)...
Abstract Vascular dementia (VaD), caused by stroke or small vessel disease, is the second‐most common type of after Alzheimer's disease (AD). Donepezil an acetylcholinesterase inhibitor that currently used in patients with mild to moderate AD, and has recently been shown improve cognitive performance VaD. In this study, we evaluated effects donepezil on VaD, investigated underlying molecular mechanisms action. VaD was established ligation bilateral carotid artery occlusion (BCCAO). Executive...