A5101451007- Tuberculosis Research and Epidemiology
- Immune Cell Function and Interaction
- Mycobacterium research and diagnosis
- T-cell and B-cell Immunology
- Diagnosis and treatment of tuberculosis
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Immune cells in cancer
- MicroRNA in disease regulation
- Microbial infections and disease research
- Pharmacological Effects of Natural Compounds
- Galectins and Cancer Biology
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- Pneumocystis jirovecii pneumonia detection and treatment
- interferon and immune responses
- Toxoplasma gondii Research Studies
- Phagocytosis and Immune Regulation
- Signaling Pathways in Disease
- Toxin Mechanisms and Immunotoxins
- Vitamin D Research Studies
- Veterinary medicine and infectious diseases
Chinese PLA General Hospital
2007-2022
The 309th Hospital of Chinese People's Liberation Army
2009-2018
Heilongjiang Center for Tuberculosis Control and Prevention
2013-2018
Beijing Haidian Hospital
2010-2016
PLA 306 Hospital
2012-2013
Peking University
2011-2012
Army Medical University
2005-2010
Chongqing Three Gorges University
2001
University of Nebraska–Lincoln
1999
University of Bern
1995-1998
Rationale: Mucosal-associated invariant T (MAIT) cells have been proven to play an important role in host defense against mycobacterial infection animal models; however, the functional of MAIT patients with active tuberculosis (TB) is still largely unknown.Objectives: To understand clinical features and functions TB.Methods: were analyzed pulmonary TB, tuberculous pleurisy, peritonitis by flow cytometry. The compared between TB healthy control subjects.Measurements Main Results: frequency...
Macrophage apoptosis is a host innate defense mechanism against tuberculosis (TB). In this study, we found that percentage of apoptotic cells in peripheral blood monocytes from patients with active TB was lower than healthy controls (p<0.001). To understand whether microRNAs can modulate monocytes, investigated differentially expressed TB. miR-582-5p mainly and upregulated The THP-1 transfected mimics significantly those negative control microRNA (p<0.001), suggesting could inhibit...
Abstract The functions of MAIT cells at the site Mycobacterium tuberculosis infection in humans are still largely unknown. In this study, phenotypes and immune response from tuberculous pleural effusions peripheral blood were investigated. had greatly enhanced IFN-γ, IL-17F granzyme B compared with those blood. level IFN-γ was inversely correlated extent (p = 0.0006). To determine whether cytokines drive responses infection, role IL-1β, IL-2, IL-7, IL-12, IL-15 IL-18 Blockade IL-12 or led to...
Strains of Mycoplasma mycoides subsp. small colony (SC) type, the agent contagious bovine pleuropneumonia (CBPP), were analysed with respect to polymorphism distribution a newly discovered insertion element, IS1296, on chromosome. Analysis 64 strains isolated from Europe, Africa and Australia, including four vaccine type strain PG1, revealed ten different IS patterns, forming two main clusters. The European originated outbreaks CBPP in countries, various other sources such as semen preputial...
To understand functional role of PD-1-expressing MAIT cells during tuberculosis infection in humans, sorted PD-1+ and PD-1- from pleural effusions patients with were subjected to transcriptome sequencing. analysed by flow cytometry their phenotypic features investigated. Transcriptome sequencing identified 144 genes that differentially expressed between tuberculous CXCL13 was the gene highest fold difference. The level associated extent TB humans. had increased production IL-21 as determined...
Etiologic diagnoses of lower respiratory tract infections (LRTI) have been relying primarily on bacterial cultures that often fail to return useful results in time. Although DNA-based assays are more sensitive than detecting pathogens, the molecular inconsistent and challenged by doubts false positives, such as those due system- environment-derived contaminations. Here we report a nationwide cohort study 2986 suspected LRTI patients across P. R. China. We compared performance assay qLAMP...
CD244 (2B4) is a member of the signaling lymphocyte activation molecule (SLAM) family immune cell receptors and it plays an important role in modulating NK CD8+ T immunity. In this study, we investigated expression function CD244/2B4 on CD4+ cells from active TB patients latent infection individuals. Active had significantly elevated M. tuberculosis antigen-specific compared with The frequencies CD244/2B4-expressing were higher retreatment than new patients. Compared CD244/2B4-dull -middle...
With the aim of characterizing specific immunogenic proteins Mycoplasma mycoides subsp. small colony (SC) type, aetiological agent contagious bovine pleuropneumonia, a gene encoding major protein 72 kDa named P72 was cloned and expressed in Escherichia coli. The same apparent molecular mass as that produced by parent strain. predicted P72, based on DNA-deduced amino acid sequence, 61.118 kDa, significantly lower than endogenous or recombinant SDS-PAGE. Analysis sequence revealed typical...