A5038628097- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Streptococcal Infections and Treatments
- Otolaryngology and Infectious Diseases
- Viral Infections and Immunology Research
- Microbial infections and disease research
- Animal health and immunology
- Animal Disease Management and Epidemiology
- Bacteriophages and microbial interactions
- Trace Elements in Health
- Clostridium difficile and Clostridium perfringens research
- Peptidase Inhibition and Analysis
- Heavy Metal Exposure and Toxicity
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Pancreatitis Pathology and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Infective Endocarditis Diagnosis and Management
- Virology and Viral Diseases
- Amoebic Infections and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Coccidia and coccidiosis research
- Dialysis and Renal Disease Management
- Autophagy in Disease and Therapy
Heilongjiang Bayi Agricultural University
2015-2025
Ministry of Agriculture and Rural Affairs
2023-2024
Guangzhou Medical University Cancer Hospital
2024
Guangzhou Medical University
2024
Heilongjiang Academy of Land Reclamation Sciences
2009
Porcine epidemic diarrhoea virus (PEDV) is an emerging and re-emerging epizootic of swine that causes substantial economic losses to the pig industry in China other countries. The variations virus, its co-infections with enteric viruses, have contributed poor control PEDV infection. In current study, a broad epidemiological investigation was carried out 22 provinces or municipalities during 2015-2018. viruses causing co-infection genetic diversity S1 gene were also analysed. results...
To trace evolution of canine parvovirus-2 (CPV-2), a total 201 stool samples were collected from dogs with diarrhea in Heilongjiang province northeast China May 2014 to April 2015. The presence CPV-2 the was determined by PCR amplification VP2 gene (568 bp) CPV-2. results revealed that 95 (47.26%) positive for CPV-2, and they showed 98.8%–100% nucleotide identity 97.6%–100% amino acid identity. Of CPV-2-positive samples, types new2a (Ser297Ala), new2b 2c accounted 64.21%, 21.05%, 14.74%,...
The emerging Porcine circovirus type 3 (PCV3) is associated with porcine dermatitis and nephropathy syndrome, reproductive failure cardiac multisystemic inflammation. To trace the prevalence evolution of PCV3 in pigs respiratory diseases or digestive China, 616 samples were collected from 21 provinces municipalities China 2015 to 2017. All analysed PCR a cap-gene-based phylogeny. results indicated that positive rate was 12.2% (75/616) at sample level; 24.1% (42/174) farm 10.4% (50/480)...
Subversion of the host cell cycle to facilitate viral replication is a common feature coronavirus infections. Coronavirus nucleocapsid (N) protein can modulate cycle, but mechanistic details remain largely unknown. Here, we investigated effects manipulation porcine epidemic diarrhea virus (PEDV) N on and influence replication. Results indicated that PEDV induced Vero E6 arrest at S-phase, which promoted (P < 0.05). S-phase was dependent nuclear localization signal S71NWHFYYLGTGPHADLRYRT90...
In an effort to trace the evolution of porcine epidemic diarrhea virus (PEDV), S1 and ORF3 genes viruses identified in 41 pig farms from seven regions (North, Northeast, Northwest, Central, East, South West, South, respectively) China 2015 were sequenced analyzed. Sequence analysis revealed that 29 our study exhibited nucleotide homologies 98.2%-100% 96.6%-100%, respectively; these two low sequence similarities with classical CV777 strain early Chinese LZC. Phylogenetic indicated PEDV...
Feline infectious peritonitis (FIP) is a fatal disease of wild and domestic cats, the occurrence FIP frequently reported in China. To trace evolution type I II feline coronavirus China, 115 samples ascetic fluid from FIP-suspected cats 54 fecal clinically healthy were collected veterinary hospitals The presence FCoV was detected by RT-PCR targeting 6b gene. results revealed that total 126 (74.6%, 126/169) positive for FCoV: 75.7% (87/115) FCoV, 72.2% (39/54) FCoV. Of FCoV-positive samples,...
Background Chronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality globally, with significant disparities in burden across countries socioeconomic regions. Despite advancements public health, the global COPD remains substantial, particularly low- middle-income countries. This study aims to provide comprehensive analysis global, regional, national trends COPD-related prevalence, deaths, disability-adjusted life years (DALYs) from 1990 2021 using an...
Recently, porcine deltacoronavirus (PDCoV) has been proven to be associated with enteric disease in piglets. Diagnostic tools for serological surveys of PDCoV remain the developmental stage when compared those other coronaviruses. In our study, an indirect enzyme-linked immunosorbent assay (ELISA) (rPDCoV-N-ELISA) was developed detect antibodies against using a histidine-tagged recombinant nucleocapsid (N) protein as antigen. The rPDCoV-N-ELISA did not cross-react antisera epidemic diarrhea...
Variants of coronavirus porcine epidemic diarrhea virus (PEDV) frequently emerge, causing an incomplete match between the vaccine and variant strains, which affects efficacy. Designing vaccines with rapidly replaceable antigens high efficacy is a promising strategy for prevention infection PEDV strains. In our study, three different types self-assembled nanoparticles (nps) targeting receptor-binding N-terminal domain (NTD) C-terminal (CTD) S1 protein, named NTDnps, CTDnps, NTD/CTDnps, were...
To trace the evolution of canine coronavirus (CCoV), 201 stool samples from diarrheic dogs in northeast China were subjected to reverse transcription-polymerase chain reactions (RT-PCRs) targeting partial M and S genes CCoV, followed by an epidemiological analysis. gene RT-PCRs showed that 28.36% (57/201) positive for CCoV; 57 samples, CCoV-I CCoV-II accounted 15.79% (9/57) 84.21% (48/57), respectively. A sequence comparison revealed nucleotide homologies 88.4%-100% among CCoV strains,...
Canine kobuviruses (CaKVs) are newly recognized picornaviruses that have been recently detected in dogs the U.S.A., Italy, U.K., Republic of Korea and Tanzania. To trace evolution CaKV strains, a total 201 fecal samples from rectal swabs diarrheic dogs, which were obtained May 2014 to April 2015 northeast China, by reverse transcription-PCR targeting partial (504 bp) fragment 3D gene. Furthermore, phylogenetic analysis strains identified China was conducted based on gene sequence. The...
Vero cells are highly susceptible to many viruses in humans and animals, its membrane proteins (MPs) responsible for virus entry. In our study, the MP proteome of was investigated using a shotgun LC-MS/MS approach. Six hundred twenty-seven proteins, including total 1839 peptides, were identified samples cells. 627 307 (48.96%) annotated terms biological process gene ontology (GO) categories; 356 (56.78%) molecular function GO 414 (66.03%) cellular components categories. Of seventeen had been...
Porcine astrovirus (PAstV) is broadly distributed globally and exists as at least five distinct genotypes. PAstV, which was recently identified an important pathogen of diarrhea in piglets, widely China. However, few studies have investigated the coinfection genetic characterization PAstV diarrheic piglets In this study, 89 PAstV-positive samples were 543 China from 2015 to 2018, 75.28% (67/89) coinfected with three different porcine pathogens, while none positive for only. Among samples,...
The plasma proteome of healthy dairy cattle and those with footrot was investigated using a shotgun LC-MS/MS approach. In total, 648 proteins were identified in samples, which 234 non-redundant 123 high-confidence proteins; 712 from 272 138 proteins. showed significant differences between samples molecular weight, isoelectric points the Gene Ontology categories. 22 found that may differentiate two sets proteins, 16 potential differential expression (PDE) involved immunoglobulins, innate...