A5025513246- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- Bacteriophages and microbial interactions
- Heavy Metal Exposure and Toxicity
- Animal health and immunology
- Microbial infections and disease research
- Botanical Studies and Applications
- Autophagy in Disease and Therapy
- Trace Elements in Health
- Glycosylation and Glycoproteins Research
- Plant and Fungal Interactions Research
- interferon and immune responses
- Viral Infections and Vectors
- Viral Infectious Diseases and Gene Expression in Insects
Heilongjiang Bayi Agricultural University
2015-2024
Zhejiang A & F University
2022-2024
Porcine epidemic diarrhoea virus (PEDV) is an emerging and re-emerging epizootic of swine that causes substantial economic losses to the pig industry in China other countries. The variations virus, its co-infections with enteric viruses, have contributed poor control PEDV infection. In current study, a broad epidemiological investigation was carried out 22 provinces or municipalities during 2015-2018. viruses causing co-infection genetic diversity S1 gene were also analysed. results...
To trace evolution of canine parvovirus-2 (CPV-2), a total 201 stool samples were collected from dogs with diarrhea in Heilongjiang province northeast China May 2014 to April 2015. The presence CPV-2 the was determined by PCR amplification VP2 gene (568 bp) CPV-2. results revealed that 95 (47.26%) positive for CPV-2, and they showed 98.8%–100% nucleotide identity 97.6%–100% amino acid identity. Of CPV-2-positive samples, types new2a (Ser297Ala), new2b 2c accounted 64.21%, 21.05%, 14.74%,...
The emerging Porcine circovirus type 3 (PCV3) is associated with porcine dermatitis and nephropathy syndrome, reproductive failure cardiac multisystemic inflammation. To trace the prevalence evolution of PCV3 in pigs respiratory diseases or digestive China, 616 samples were collected from 21 provinces municipalities China 2015 to 2017. All analysed PCR a cap-gene-based phylogeny. results indicated that positive rate was 12.2% (75/616) at sample level; 24.1% (42/174) farm 10.4% (50/480)...
Subversion of the host cell cycle to facilitate viral replication is a common feature coronavirus infections. Coronavirus nucleocapsid (N) protein can modulate cycle, but mechanistic details remain largely unknown. Here, we investigated effects manipulation porcine epidemic diarrhea virus (PEDV) N on and influence replication. Results indicated that PEDV induced Vero E6 arrest at S-phase, which promoted (P < 0.05). S-phase was dependent nuclear localization signal S71NWHFYYLGTGPHADLRYRT90...
Feline infectious peritonitis (FIP) is a fatal disease of wild and domestic cats, the occurrence FIP frequently reported in China. To trace evolution type I II feline coronavirus China, 115 samples ascetic fluid from FIP-suspected cats 54 fecal clinically healthy were collected veterinary hospitals The presence FCoV was detected by RT-PCR targeting 6b gene. results revealed that total 126 (74.6%, 126/169) positive for FCoV: 75.7% (87/115) FCoV, 72.2% (39/54) FCoV. Of FCoV-positive samples,...
ABSTRACT As a porcine alphacoronavirus , epidemic diarrhea virus (PEDV) frequently undergoes mutations that significantly reduce the effectiveness of current prevention and control strategies, leading to recurrent outbreaks in China. This study investigates genetic evolution mutation patterns S1 protein characterize PEDV variation Genetic evolutionary analysis 804 genes, including 620 Chinese strains, revealed 78.06% strains belong G2a‐subgroup, further divided into seven branches (G2a‐Clade...
Recently, porcine deltacoronavirus (PDCoV) has been proven to be associated with enteric disease in piglets. Diagnostic tools for serological surveys of PDCoV remain the developmental stage when compared those other coronaviruses. In our study, an indirect enzyme-linked immunosorbent assay (ELISA) (rPDCoV-N-ELISA) was developed detect antibodies against using a histidine-tagged recombinant nucleocapsid (N) protein as antigen. The rPDCoV-N-ELISA did not cross-react antisera epidemic diarrhea...
Variants of coronavirus porcine epidemic diarrhea virus (PEDV) frequently emerge, causing an incomplete match between the vaccine and variant strains, which affects efficacy. Designing vaccines with rapidly replaceable antigens high efficacy is a promising strategy for prevention infection PEDV strains. In our study, three different types self-assembled nanoparticles (nps) targeting receptor-binding N-terminal domain (NTD) C-terminal (CTD) S1 protein, named NTDnps, CTDnps, NTD/CTDnps, were...
Lead (Pb) may damage the immune function in human and animal. Selenium (Se) has antagonistic effects on Pb. In our study, brown layer chickens were randomly allocated to control group, Se group (1 mg/kg Se), Se+Pb 350 Pb), Pb (350 Pb). The sacrificed 90th day; spleen tissues subjected observation of ultrastructure detection spleen-related indexes. results revealed that expression levels cytokines IL-1 TNF-α significantly increased, IL-2 INF-γ decreased; activities antioxidant enzyme GPX, SOD...
To trace the evolution of canine coronavirus (CCoV), 201 stool samples from diarrheic dogs in northeast China were subjected to reverse transcription-polymerase chain reactions (RT-PCRs) targeting partial M and S genes CCoV, followed by an epidemiological analysis. gene RT-PCRs showed that 28.36% (57/201) positive for CCoV; 57 samples, CCoV-I CCoV-II accounted 15.79% (9/57) 84.21% (48/57), respectively. A sequence comparison revealed nucleotide homologies 88.4%-100% among CCoV strains,...
Canine kobuviruses (CaKVs) are newly recognized picornaviruses that have been recently detected in dogs the U.S.A., Italy, U.K., Republic of Korea and Tanzania. To trace evolution CaKV strains, a total 201 fecal samples from rectal swabs diarrheic dogs, which were obtained May 2014 to April 2015 northeast China, by reverse transcription-PCR targeting partial (504 bp) fragment 3D gene. Furthermore, phylogenetic analysis strains identified China was conducted based on gene sequence. The...
Porcine astrovirus (PAstV) is broadly distributed globally and exists as at least five distinct genotypes. PAstV, which was recently identified an important pathogen of diarrhea in piglets, widely China. However, few studies have investigated the coinfection genetic characterization PAstV diarrheic piglets In this study, 89 PAstV-positive samples were 543 China from 2015 to 2018, 75.28% (67/89) coinfected with three different porcine pathogens, while none positive for only. Among samples,...
Transmissible gastroenteritis virus (TGEV) is a coronavirus (CoV) that major pathogenity of viral enteritis and diarrhea in suckling piglets, causing high morbidity mortality. In this study, TGEV strain HQ2016 was isolated from northeast China characterized its genome sequence pathogenicity. The phylogenetic analysis indicated the more similar to Purdue cluster than Miller cluster. Both recombination based on each structural non-structural gene revealed no event strain. Experimental...
Porcine epidemic diarrhea virus (PEDV) causes and vomiting in piglets, leading to a mortality rate of 100%. Due the high frequency mutation, it is important monitor evolution PEDV develop potential vaccine candidates. In this study, two strains (ZJ2022 ZQ2022) were identified by PCR. These subsequently isolated, their genome sequences, growth characteristics, pathogenicity compared. Phylogenetic recombination analyses revealed that both belonged GIIa-subgroup, ZQ2022 was as recombinant...
Recently, parainfluenza virus 5 (PIV5) infection has been increasingly reported in mammals. In this study, five PIV5 strains were isolated from diarrhea-affected piglets four provinces or municipalities China. An F-gene-based phylogenetic tree indicated that the closely related to strain ZJQ-221 a lesser panda China, and 1168-1 dog South Korea. The new isolates differed genetically other pig, calf, rhesus macaque kidney cells, human, reference strains. Our study reveals presence of...
ABSTRACT Limited information on the virome and bacterial community hampers our ability to discern systemic ecological risk factors that cause cattle diarrhea, which has become a pressing issue in control of disease. A total 110 viruses, 1,011 genera, 322 complete viral genomes were identified from 70 sequencing samples mixed with 1,120 fecal 58 farms northeast China. For diarrheic samples, varied terms composition, abundance, diversity, geographic distribution relation different...