A5026291302- Mitochondrial Function and Pathology
- Alcohol Consumption and Health Effects
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Enzyme Structure and Function
- Heavy metals in environment
- ATP Synthase and ATPases Research
- Chromium effects and bioremediation
- Photosynthetic Processes and Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Metabolism and Genetic Disorders
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and Molecular Research
- Metalloenzymes and iron-sulfur proteins
- Plant Stress Responses and Tolerance
- Biotin and Related Studies
- Geochemistry and Elemental Analysis
- Eicosanoids and Hypertension Pharmacology
- Bacterial biofilms and quorum sensing
- Mercury impact and mitigation studies
- Gut microbiota and health
- Biochemical effects in animals
- Cancer Research and Treatments
- Essential Oils and Antimicrobial Activity
- Metal-Catalyzed Oxygenation Mechanisms
Instituto Nacional de Cardiología
2014-2024
Instituto Nacional de Cardiologia
2008-2024
Universidad Michoacana de San Nicolás de Hidalgo
1997-2013
Purdue University West Lafayette
2001-2002
AS‐30D hepatoma cells, a highly oxidative and fast‐growing tumor line, showed glucose‐induced fructose‐induced inhibition of phosphorylation (the Crabtree effect) 54% 34%, respectively. To advance the understanding underlying mechanism this process, effect 5 m glucose or 10 fructose on intracellular concentration several metabolites was determined. The addition lowered P i (40%), ATP (53%) concentrations, decreased cytosolic pH (from 7.2 to 6.8). Glucose increased content AMP (30%),...
Aims: To analyse the production of different metabolites by dark-grown Euglena gracilis under conditions found to render high cell growth. Methods and Results: The combination glutamate (5 g l−1), malate (2 l−1) ethanol (10 ml (GM + EtOH); (7·15 l−1); or (8·16 glucose (10·6 NH4Cl (1·8 as carbon nitrogen sources, promoted an increase 5·6, 3·7 2·6-fold, respectively, in biomass concentration comparison with (GM). In turn, α-tocopherol after 120 h identified LC-MS was ± 0·2, 2·4 0·1 2 mg [g dry...
Aldehyde dehydrogenases (ALDHs) are involved in the detoxification of aldehydes generated as byproducts lipid peroxidation. In this work, it was determined that, among three most studied human ALDH isoforms, ALDH2 showed highest catalytic efficiency for oxidation acrolein, 4-hydroxy-2-nonenal (4-HNE), and malondialdehyde. ALDH1A1 also exhibited significant activity with these substrates, whereas ALDH3A1 only 4-HNE. sensitive isoform to irreversible inactivation by compounds. Remarkably,...
Ethanol accumulation during fermentation contributes to the toxic effects in Saccharomyces cerevisiae, impairing its viability and fermentative capabilities. The iron-sulfur (Fe-S) cluster biogenesis is encoded by ISC genes. Reactive oxygen species (ROS) generation associated with iron release from Fe-S-containing enzymes. We evaluated ethanol toxicity, ROS generation, antioxidant response mitochondrial integrity S. cerevisiae mutants. These mutants showed an impaired tolerance ethanol....
Mitochondrial calcium overload has been implicated in the irreversible damage of reperfused heart. Accordingly, we studied effect an oxygen-bridged dinuclear ruthenium amine complex (Ru360), which is a selective and potent mitochondrial uniporter blocker, on dysfunction matrix free-calcium concentration mitochondria isolated from rat hearts. The perfusion Ru360 maintained oxidative phosphorylation prevented opening permeability transition pore We found that only partially inhibited...
The effect of increased expression or reconstitution the mitochondrial inhibitor protein (IF1) on dimer/monomer ratio (D/M) rat liver and bovine heart F1F0-ATP synthase was studied. 2-fold IF1 in AS-30D hepatoma mitochondria correlated with a 1.4-fold increase D/M ATP extracted digitonin as determined by blue native electrophoresis averaged densitometry analyses. Removal from submitochondrial particles F1F0-ATPase activity decreased synthase. Reconstitution recombinant into devoid inhibited...
Biogenesis and recycling of iron-sulfur (Fe-S) clusters play important roles in the iron homeostasis mechanisms involved mitochondrial function. In Saccharomyces cerevisiae, Fe-S are assembled into apoproteins by cluster machinery (ISC). The aim present study was to determine effects ISC gene deletion consequent release under oxidative stress conditions on functionality S. cerevisiae. Reactive oxygen species (ROS) generation, caused H2O2, menadione, or ethanol, associated with a loss...
The F1FO and F1-ATPase complexes of Paracoccus denitrificans were isolated for the first time by ion exchange, gel filtration, density gradient centrifugation into functional native preparations. liposome-reconstituted holoenzyme preserves its tight coupling between F1 FO sectors, as evidenced high sensitivity to inhibitors venturicidin diciclohexylcarbodiimide. Comparison N-terminal sequencing band profile in SDS-PAGE preparations showed a novel 11-kDa protein addition 5 canonical α, β, γ,...
The in vitro Entamoeba histolytica pyruvate:ferredoxin oxidoreductase (EhPFOR) kinetic properties and the effect of oxidative stress on glycolytic pathway enzymes fluxes live trophozoites were evaluated. EhPFOR showed a strong preference for pyruvate as substrate over other oxoacids. enzyme was irreversibly inactivated by long period saturating O(2) exposure (IC(50) 0.034 mm), whereas short-term (< 30 min) leading to > 90% inhibition allowed partial restoration addition Fe(2+). CoA...
Under physiological conditions, cells produce low basal levels of reactive oxygen species (ROS); however, in pathologic conditions ROS production increases dramatically, generating high concentrations toxic unsaturated aldehydes. Aldehyde dehydrogenases (ALDHs) are responsible for detoxification these aldehydes protecting the cell. Due to relevance enzymes, it is important design strategies modulate their activity. It was previously reported that omeprazole activation ALDH1A1 protected...
The effect of varying the Mg2+concentration on 2-oxoglutarate dehydrogenase (2-OGDH) activity and rate oxidative phosphorylation rat heart mitochondria was studied. ionophore A23187 used to modify mitochondrial free Mg2+ concentration. Half-maximal stimulation (K0.5) ATP synthesis by obtained with 0.13 ± 0.02 mm (n = 7) succinate (+rotenone) 0.48 mm(n 6) (2-OG) as substrates. Similar K0.5 values were found for NAD(P)H formation, generation membrane potential, state 4 respiration 2-OG. In...
Aldehyde dehydrogenases are isolated as dimers or tetramers but have essentially identical structures. The homotetramer (ALDH1 ALDH2) is a dimer of (A−B + C−D). In the tetrameric enzyme, Ser500 from subunit "D" interacts with Arg84, conserved residue, "A". dimeric ALDH3 form, interaction cannot exist. It has been proposed that formation tetramer prevented by presence C-terminal tail in not present ALDH1 2. To understand forces maintain tetramer, deletion ALDH3, addition different tails...
Aldehyde dehydrogenases are general detoxifying enzymes, but there also isoenzymes that involved in specific metabolic pathways different organisms. Two of these enzymes Escherichia coli lactaldehyde (ALD) and phenylacetaldehyde (PAD), which participate the metabolism fucose phenylalanine, respectively. These isozymes share some properties with better characterized mammalian have kinetic unique. It was possible to thread sequences into known ones for understand structural differences. Both...
High‐molecular‐mass PC complexes (PC‐HMWCs) constituted by phytochelatins (PCs), cadmium and sulfide are synthesized several organisms after exposure to cadmium. In this study, PC‐HMWCs were isolated from photoheterotrophic Euglena gracilis purified homogeneity, resulting in compounds of molecular mass 50–380 kDa depending on the CdCl 2 sulfate concentrations culture medium. contrast with plants some yeasts, E. mainly comprise (57–75%) monothiol molecules (Cys, γ‐glutamylcysteine, GSH) and,...
Mitochondrial aldehyde dehydrogenase (ALDH2) has been proposed as a key enzyme in cardioprotection during ischemia-reperfusion processes. This proposal led to the search for activators of ALDH2 with aim develop cardioprotective drugs. Alda-1 was first activator identified and its effect extensively proven vivo; however, mechanism activation is not fully understood. A crystallographic study showed that binds entrance aldehyde-binding site; therefore, should essence be an inhibitor. In present...
As options to treat recalcitrant bacterial infections which are increasingly limited due multidrug-resistant strains, searching for new, effective antibacterial compounds is necessary. One strategy generate treatment alternatives by drug repurposing.In this work, phenotypic microarrays were used the screening of miscellaneous against growth and biofilm formation Acinetobacter baumannii, an important emergent opportunistic pathogen. The results showed that phenothiazine derivatives, such as...
The modulation of aldehyde dehydrogenase (ALDH) activity has been suggested as a promising option for the prevention or treatment many diseases. To date, only few activating compounds ALDHs have described. In this regard, N-(1,3-benzodioxol-5-ylmethyl)-2,6-dichlorobenzamide used to protect heart against ischemia/reperfusion damage. search new modulating ALDH molecules, binding capability different active site human class 1A1 (ALDH1A1) was analyzed by molecular docking, and their ability...