A5101537658- Genomics, phytochemicals, and oxidative stress
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Cell Image Analysis Techniques
- Natural product bioactivities and synthesis
- Retinoids in leukemia and cellular processes
- Antioxidant Activity and Oxidative Stress
- Cancer Genomics and Diagnostics
- Computational Drug Discovery Methods
- Curcumin's Biomedical Applications
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Skin Protection and Aging
- Phytochemicals and Antioxidant Activities
- Protein Hydrolysis and Bioactive Peptides
- Bladder and Urothelial Cancer Treatments
- Circadian rhythm and melatonin
- Signaling Pathways in Disease
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Food, Nutrition, and Cultural Practices
- Advanced Fluorescence Microscopy Techniques
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
Rutgers, The State University of New Jersey
2014-2024
Johnson University
2015-2019
Memorial Sloan Kettering Cancer Center
2009-2012
Kettering University
2011-2012
Abstract Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental carcinogens accountable to developing skin cancers. Recently, we reported that exposure benzo[a]pyrene (B[a]P), a common PAH, causes epigenetic and metabolic alterations in the initiation, promotion progression of non-melanoma cancer (NMSC). As follow-up investigation, this study examines how dietary triterpenoid ursolic acid (UA) regulates B[a]P-driven pathways SKH-1 hairless mice. Our results show UA intercepts...
Importance of the field: Monitoring cell viability in vitro is critical many areas biomedical research, and ultimate goal drug discovery ability to predict vivo toxicology candidates based on their toxicity profile vitro. Over last decade, contribution high-throughput screening toward this has been tremendous, providing screen compounds parallel against multiple types. However, toxic effects uncovered during clinical trials are by far main reason for failure. same period, our understanding...
Caspases are central to the execution of programmed cell death, and their activation constitutes biochemical hallmark apoptosis. In this article, authors report successful adaptation a high-content assay method using DEVDNucView488 fluorogenic substrate, for first time, they show caspase in live cells induced by either drugs or siRNA. The substrate was found be nontoxic over an exposure period several days, during which demonstrate automated imaging quantification same population as function...
Abstract Nonmelanoma skin cancers (NMSCs) are the most common type of cancers. Major risk factors for NMSCs include exposure to ultraviolet (UV) irradiation. Ursolic acid (UA) is a natural triterpenoid enriched in blueberries and herbal medicinal products, possess anticancer activities. This study focuses on impact UA epigenomic, genomic mechanisms prevention UVB‐mediated NMSC. CpG methylome RNA transcriptome alterations early, promotion late stages treated UVB‐induced NMSC SKH‐1 hairless...
Abstract Non-melanoma skin cancer (NMSC) is the most common in world. Environmental exposure to carcinogens one of major causes NMSC initiation and progression. In current study, we utilized a two-stage carcinogenesis mouse model generated by sequential cancer-initiating agent benzo[a]pyrene (BaP) promoting 12-O-tetradecanoylphorbol-13-acetate (TPA), study epigenetic, transcriptomic metabolic changes at different stages during development NMSC. BaP/TPA caused significant alterations DNA...
Sulforaphane (SFN), a potent antioxidant and antiinflammatory agent, has been shown to protect against cancers especially at early stages. However, how SFN affects UVB-mediated epigenome/DNA methylome transcriptome changes in skin photodamage not fully assessed. Herein, we investigated the transcriptomic DNA methylomic during tumor initiation, promotion, progression its impact reversal by using next-generation sequencing (NGS) technology. The results show that reduced incidence number. SFN's...
microRNAs (miRNAs) are evolutionary conserved, small endogenous non-coding, RNA molecules. Although their mode of action has been extensively studied, little is known about biogenesis. As altered expression implicated in many diseases, molecules that would modulate sought after. They generated through the concerted several complexes which promote transcription, maturation, export, trafficking, and loading mature miRNA into silencing complexes. An increasing number studies have suggested each...
Automated microscopy was introduced two decades ago and has become an integral part of the discovery process as a high-content screening platform with noticeable challenges in executing cell-based assays. It would be interest to use it screen for reversers transformed cell phenotype. In this report, we present data obtained from optimized assay that identifies compounds reverse phenotype induced NIH-3T3 cells by expressing novel oncogene, KP, resulting fusion between platelet derived growth...
RNA interference technology is becoming an integral tool for target discovery and validation.; With perhaps the exception of only few studies published using arrayed short hairpin (shRNA) libraries, most reports have been either against pooled siRNA or shRNA, libraries. For this purpose, we developed a workflow performed genome-scale shRNA lethality screen TRC1 library in HeLa cells. The resulting targets would be valuable resource candidates toward better understanding cellular homeostasis....
MicroRNAs (miRNAs) are evolutionary conserved noncoding molecules that regulate gene expression. They influence a number of diverse biological functions, such as development and differentiation. However, their dysregulation has been shown to be associated with disease states, cancer. Genes pathways regulating biogenesis remain unknown highly sought after. For this purpose, we have validated multiplexed high-content assay strategy screen for modulators. Here, describe its implementation makes...
Abstract Nonmelanoma skin cancer (NMSC) is the most common burden on U.S. population. Environmental exposure to chemical carcinogens one of major causes NMSC initiation, promotion, and progression. Ursolic acid (UA) a naturally abundant pentacyclic triterpenoid showing anticancer potentials against diverse cancers. In current study, we developed two-stage carcinogenesis model in SKH1 hairless mice by administering cancer-initiating agent benzo[a]pyrene (B[a]P) promoting...
Abstract Despite extensive drug discovery efforts, drug-candidate failure and patients relapsing in the clinic remain as persistent problems. While insufficient drug-gene engagement leads to failure, de novo escape mutations give rise relapsing, calling need for systemic studies on how genes influence responsiveness. Towards this end, we have explored a functional short hairpin RNA (shRNA) based genomic screening platform aimed at interrogating assessing its consequences signaling pathways....