A5102784586- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- Streptococcal Infections and Treatments
- Aquaculture disease management and microbiota
- Toxoplasma gondii Research Studies
- vaccines and immunoinformatics approaches
- Endoplasmic Reticulum Stress and Disease
- Metabolism and Genetic Disorders
- Animal Virus Infections Studies
- Biochemical and Molecular Research
- Escherichia coli research studies
- Immune Cell Function and Interaction
- Biochemical Acid Research Studies
- Peroxisome Proliferator-Activated Receptors
- Antimicrobial Peptides and Activities
- Poxvirus research and outbreaks
- Vibrio bacteria research studies
- Bacterial Infections and Vaccines
- Bacillus and Francisella bacterial research
- Transgenic Plants and Applications
- Salmonella and Campylobacter epidemiology
- Toxin Mechanisms and Immunotoxins
- Viral Infections and Vectors
- Respiratory viral infections research
Harbin Veterinary Research Institute
2007-2024
Chinese Academy of Agricultural Sciences
2007-2024
Smallpox is a highly contagious human disease caused by the variola virus. Although was eliminated in 1979 due to its nature and historical pathogenicity, with mortality rate of up 30%, this virus an important candidate for biological weapons. Currently, vaccines are critical measures prevent infection spread. In study, we designed peptide vaccine using immunoinformatics tools, which have potential activate immunity against efficiently. The design peptides derives from vaccine-candidate...
Infection by Mycoplasma bovis (M. bovis) can induce diseases, such as pneumonia and otitis media in young calves mastitis arthritis older animals. Here, we report the finished annotated genome sequence of M. strain Hubei-1, a isolated 2008 that caused calf on Chinese farm. The Hubei-1 contains single circular chromosome 953,114 bp with 29.37% GC content. We identified 803 open reading frames (ORFs) occupy 89.5% genome. While 34 ORFs were specific, 662 had orthologs type PG45 Genome analysis...
Mycoplasma hyopneumoniae is a colonizing respiratory pathogen that can cause great economic losses to the pig industry worldwide. Although putative virulence factors have been reported, pathogenesis of this species remains unclear. Here, we used virulent M. strain 168 infect swine tracheal epithelial cells (STEC) identify infection-associated by two-dimensional electrophoresis (2-DE). Whole proteins were obtained and compared with samples cultured in broth. Six differentially expressed an...
Mycoplasmas persist in the host for a long time, suggesting that they possess mechanisms immune evasion. Factor H is negative regulator of complement system, which binds to cells avoid unexpected activation. In this study, we revealed many mycoplasmas, such as Mycoplasma hyopneumoniae, hyorhinis, hyosynoviae, gallisepticum, pneumoniae, genitalium, flocculare, and bovis could hijack factor present themselves tissue thus escape from attack. Furthermore, mechanism recruiting was identified M....
Mycoplasma gallisepticum is an avian respiratory and reproductive tract pathogen that has a significant economic impact on the poultry industry worldwide. Although membrane proteins of spp. are thought to play crucial roles in host interactions, very few have had their biochemical function defined. In this study, we found GroEL protein (heat shock 60) could induce apoptosis peripheral blood mononuclear cells, underlying molecular mechanism was further determined. The gene from cloned...
Abstract For the development of a competitive ELISA (cELISA) to detect serum antibodies against Mycoplasma mycoides subsp. Mycoides ( Mmm ) (strain PG1), causative agent contagious bovine pleuropneumonia (CBPP), all proteins this pathogen were analyzed. Then, specific extracellular region transmembrane protein with potential for diagnosis was identified. After that, monoclonal antibody (Mab) named 3A8 obtained using as an immunogen. Finally, cELISA established domain coating antigen, Mab...
Enzootic pneumonia caused by
Mycoplasma hyopneumoniae causes the disease porcine enzootic pneumonia, a highly contagious and chronic affecting pigs. Understanding molecular mechanisms of its pathogenicity is critical for developing effective interventions to control this swine respiratory disease. Here, we describe novel virulence mechanism by which M. interferes with host unfolded protein response (UPR) eventually facilitates bacterial adhesion infection.
The unfolded protein response (UPR) plays a crucial role in Mycoplasma hyopneumoniae (M. hyopneumoniae) pathogenesis. We previously demonstrated that M. interferes with the host UPR to foster bacterial adhesion and infection. However, underlying molecular mechanism of this modulation is unclear. Here, we report membrane Mhp271 interacts GRP78, master regulator localized porcine tracheal epithelial cells (PTECs) surface. interaction GRP78 reduces beta-defensin 2 (PBD-2) production, thereby...
Lipoic acid is a conserved cofactor necessary for the activation of several critical enzyme complexes in aerobic metabolism 2-oxoacids and one-carbon metabolism. Lipoate enzymes are key lipoic biosynthesis salvage. In this study, we found that Mycoplasma hyopneumoniae ( M. ) Mhp-Lpl, which had been previously shown to have lipoate-protein ligase activity against glycine cleavage system H protein (GcvH) vitro , did not lipoylate lipoate-dependent subunit dihydrolipoamide dehydrogenase (PdhD)....
Mycoplasma hyopneumoniae (M. hyopneumoniae) is the causative agent of pandemic pneumonia among pigs, namely, swine enzootic pneumonia. Although M. was first identified in 1965, little known regarding its metabolic pathways, which might play a pivotal role during disease pathogenesis. Lipoate an essential cofactor for enzymes important central metabolism. However, lipoate metabolism pathway definitely unclear. Here, we novel gene, lpl, encoding protein ligase genome (Mhp-Lpl). This gene...
Abstract Vaccination with inactivated bacterin is the most popular and practical measure to control enzootic pneumonia. After immunisation bacterin, Mycoplasma hyopneumoniae colonised on respiratory tract lung stimulates humoural immune responses produces IgG IgA antibodies. ELISA a widely used serological method detect M. However, commercial IgG‐ELISA kit cannot distinguish between bacterin‐induced hyperimmune sera convalescent stimulated by natural infection. SIgA‐ELISA needs collect nasal...
Mycoplasmas are minimal but notorious bacteria that infect humans and animals. These genome-reduced organisms have evolved strategies to overcome host apoptotic defense establish persistent infection. Here, using Mycoplasma bovis as a model, we demonstrate mycoplasma glycine cleavage system (GCS) H protein (GcvH) targets the endoplasmic reticulum (ER) hijack apoptosis facilitating bacterial Mechanically, GcvH interacts with ER-resident kinase Brsk2 stabilizes it by blocking its autophagic...
Abstract Background: Mycoplasma hyopneumoniae is the primary pathogen of enzootic pneumonia (EP). Vaccination with inactivated bacterin most popular and practical measure to control EP. However, these commercial vaccines have a limited effect on transmission microorganism cannot prevent colonization. Therefore, after immunization bacterin, M. colonized respiratory tract lungs stimulates humoral immune responses produce IgG IgA antibodies. ELISA widely used serological method detect kit...