A5065323812- Birth, Development, and Health
- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Stress Responses and Cortisol
- Spaceflight effects on biology
- Neuroendocrine regulation and behavior
- Cancer Cells and Metastasis
- Hormonal Regulation and Hypertension
- Diet and metabolism studies
- Muscle metabolism and nutrition
- Neuropeptides and Animal Physiology
- Neonatal Respiratory Health Research
- Angiogenesis and VEGF in Cancer
- Molecular Biology Techniques and Applications
- Protease and Inhibitor Mechanisms
- Cancer Genomics and Diagnostics
- Electrolyte and hormonal disorders
- Ubiquitin and proteasome pathways
- Receptor Mechanisms and Signaling
- Infant Nutrition and Health
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- Cerebral Palsy and Movement Disorders
- Cell Adhesion Molecules Research
- Heat shock proteins research
Université de Lille
2003-2024
Unité de Recherche Pluridisciplinaire Sport, Santé, Société
2016-2024
Université d'Artois
2021-2024
Illumina (United States)
2014-2020
Université Lille Nord de France
2009-2014
Laboratoire de Neurosciences Intégratives et Adaptatives
2011
University of California, San Diego
2004-2009
Moores Cancer Center
2007
Harvard University
2007
Laboratoire de Biologie du Développement
1994-2005
Cell metastasis is a highly dynamic process that occurs in multiple steps. Understanding this has been limited by the inability to visualize tumor cell behavior real time using animal models. Here, we employ translucent zebrafish and high-resolution confocal microscopy study how human cancer cells invade tissues, induce angiogenesis, interact with newly formed vessels. We use system metastatic gene RhoC promotes initial steps of metastasis. find expression induces primitive amoeboid-like...
Hypoxia activates genetic programs that facilitate cell survival; however, in cancer, it may promote invasion and metastasis. In this study, we show breast cancer cells cultured 1.0% O2 demonstrate changes consistent with epithelial–mesenchymal transition (EMT). Snail translocates to the nucleus, E-cadherin is lost from plasma membranes. Vimentin expression, migration, Matrigel invasion, collagen remodeling are increased. Hypoxia-induced EMT accompanied by increased expression of...
Hypoxia induces expression of the urokinase receptor (uPAR) and activates uPAR-dependent cell signaling in cancer cells. This process promotes epithelial-mesenchymal transition (EMT). uPAR overexpression cells also EMT. In this study, we tested whether may be targeted to reverse When MDA-MB 468 breast were cultured 1% O(2), increased, as anticipated. Cell-cell junctions disrupted, vimentin E-cadherin was lost from surfaces, indicating Transferring these back 21% O(2) decreased reversed signs...
Tumor-associated macrophages are known to influence cancer progression by modulation of immune function, angiogenesis, and cell metastasis, however, little is about the chemokine signaling networks that regulate this process. Utilizing CT26 colon cells RAW 264.7 as a model cellular system, we demonstrate treatment with conditioned medium induces migration, invasion metastasis. Inflammatory gene microarray analysis indicated CT26-stimulated upregulate SDF-1alpha VEGF, these cytokines...
Previous work in our laboratory led to the cloning, from same parent tumor cell line (MDA-MB-435), of two human breast cancer lines (M-4A4 and NM-2C5) with opposite metastatic phenotypes. Additional investigations revealed that nonmetastatic NM-2C5 overexpressed neutrophil collagenase, matrix metalloproteinase (MMP)-8, relative its partner. Because other studies have implicated MMP family promoting metastasis, we investigated apparently paradoxical expression MMP-8 these lines. By genetic...
Several studies have shown that maternal undernutrition leading to low birth weight predisposes offspring the development of metabolic pathologies such as obesity. Using a model prenatal 70% food restriction diet (FR30) in rat, we evaluated whether postweaning high-fat (HF) would amplify phenotype observed under standard diet. We investigated biological parameters well gene expression profile focusing on white adipose tissues (WAT) adult offspring. FR30 procedure does not worsen syndrome...
Abstract Low-density lipoprotein receptor-related protein-1 (LRP-1) is a multifunctional receptor involved in receptor-mediated endocytosis and cell signaling. In this study, we show that LRP-1 abundantly expressed severe combined immunodeficient (SCID) mouse xenografts by various human cancer lines express very low or undetectable levels of when cultured 21% O2 vitro (standard culture conditions). To test whether expression vivo may be explained hypoxia the xenografts, CL16 cells, which are...
We investigated the effects of perinatal maternal malnutrition on hypothalamo-pituitary-adrenal (HPA) axis activity in both basal and stressful conditions newborn rats at weaning. Mothers from control group were fed ad libitum. exposed to food restriction received 50% (FR50) daily intake pregnant dams during last week gestation (Pre group), lactation (Post group) or periods (PP order compare long-term gestational and/or lactational restriction. FR50 reduced body growth pups Post PP groups as...
This study used a unique xenogeneic breast cancer model to the effects of tumor cells and neighboring host upon each other in growth metastasis. It exploited species differences between interacting components determine how influenced vice versa. was found that gene expression profiles highly poorly metastatic clones from same human carcinoma changed differentially when were transferred vitro mammary gland. We describe novel sets genes, validated by human-specific probes, which induced 2...
Childhood is a period of construction the organism, during which interactions with environment and regular physical activity are necessary for maturation neuronal networks. An atypical sensorimotor childhood (due to bed-rest or neurodevelopmental disorders) impacts development neuromuscular system. A model restriction (SMR) developed in rats has shown that casting pups' hind limbs from postnatal day 1 (P1) P28 induced severe perturbation motor behavior, due muscle weakness as well...
A short abnormal polyalanine expansion in the polyadenylate-binding protein nuclear-1 (PABPN1) causes oculopharyngeal muscular dystrophy (OPMD). Mutated PABPN1 proteins accumulate as insoluble intranuclear aggregates muscles of OPMD patients. While roles nuclear polyadenylation and regulation alternative poly(A) site choice have been established, molecular mechanisms which trigger pathological defects role remain to be determined. Using exon array, for first time we identified several...
The sarcoplasmic reticulum (SR) plays an important role in calcium homeostasis. SR mishandling is described pathological conditions, such as myopathies. Here, we investigated whether the nuclear receptor subfamily 1 group D member (NR1D1, also called REV-ERBα) regulates skeletal muscle Our data demonstrate that NR1D1 deficiency mice impaired sarco/endoplasmic ATPase-dependent (SERCA-dependent) uptake. acts on homeostasis by repressing SERCA inhibitor myoregulin through direct binding to its...
Several studies indicate that maternal undernutrition sensitizes the offspring to development of metabolic disorders, such as obesity. Using a model perinatal 50% food-restricted diet (FR50), we recently reported rat neonates from undernourished mothers exhibit decreased leptin plasma levels associated with alterations hypothalamic proopiomelanocortin system. The present study aimed at examining consequences FR50 on brain-adipose axis in male neonates. quantitative RT-PCR array containing 84...
Undernutrition exposure during the perinatal period reduces growth kinetic of offspring and sensitizes it to development chronic adult metabolic diseases both in animals humans. Previous studies have demonstrated that a 50% maternal food restriction performed last week gestation lactation has short- long-term consequences male rat offspring. Pups from undernourished mothers present decreased intrauterine (IUGR) extrauterine restriction. This is associated with drastic reduction their leptin...
Oncogenic fusion genes underlie the mechanism of several common cancers. Next-generation sequencing based RNA-seq analyses have revealed an increasing number recurrent fusions in a variety However, absence publicly available gene-fusion focused data impedes comparative assessment and collaborative development novel gene detection algorithms. We generated nine synthetic poly-adenylated RNA transcripts that correspond to previously reported oncogenic fusions. These RNAs were spiked at known...
Muscle biopsies were taken from soleus and vastus lateralis before after a 60-day bed rest (BR) to examine expression changes in the regulatory proteins of thin filament contractile function. Twenty-four women separated three groups submitted BR or combined protocol resistance aerobic exercises during received supplementation amino acids BR. Ca(2+)-tension relationships established single skinned fibers identified by their myosin heavy chain troponin C isoform expressions. Expression...