Abstract As one of the most attractive methods for synthesis ordered hierarchically porous crystalline materials, soft-template method has not appeared in covalent organic frameworks (COFs) due to incompatibility surfactant self-assembly and guided crystallization process COF precursors phase. Herein, we connect soft templates backbone through ionic bonds, avoiding their incompatibilities, thus introducing an additional arrangement into anionic microporous COFs. The ion exchange is used...

Abstract Efficiently transporting extracellular electrons from microbial biofilms to the electrodes is challenging and critical in achieving high‐performance fuel cells (MFCs). In this work, we develop a simple effective method fabricate hybrid electroactive by inserting bacteria into graphene–carbon‐nanotube (G–CNT) networks (namely, G–CNT‐biofilm) as an anode for MFCs. This novel architecture greatly enhances direct electron transfer between Shewanella oneidensis electrode, due strong...

Pseudomonas aeruginosa is a biofilm-forming opportunistic pathogen which causes chronic infections in immunocompromised patients and leads to high mortality rate. It identified as common coinfecting COVID-19 causing exacerbation of illness. In our hospital, P. one the top bacteria among patients. We collected strong strain displaying small colony variant morphology from severe patient. Genomic transcriptomic sequencing analyses were performed with phenotypic validation investigate its...

The fabrication of hierarchically porous covalent organic framework (COF) aerogels is always challenging but great significance in chemistry and materials science. known COF aerogel preparation system very limited usually results composite with low crystallinity. Herein, a universal moderate synthetic strategy presented for undoped through the catalysis scandium trifluoromethanesulfonate. Due to high catalytic activity catalyst on aldimine condensation, densely distributed small crystal...

Developing crystalline porous materials with highly efficient CO2 selective adsorption capacity is one of the key challenges to carbon capture and storage (CCS). In current studies, much more attention has been paid properties for CCS, while defects, which are unavoidable ubiquitous, relatively neglected. Herein, first time, we propose a monomer-symmetry regulation strategy directional defect release achieve in situ functionalization COFs exposing uniformly distributed defect-aldehyde groups...

99TcO4– with radioactive toxicity and high mobility is a potential radiation threat to the environment organisms. Effective separation fixation of from nuclear waste are paramount importance. Herein, we proposed pre-evaluation strategy supported by single-crystal structures prepare two kinds cationic covalent organic polymers (COPs) self-adaptive effects remove 99TcO4–/ReO4– aqueous solution. The as-prepared COPs showed rapid adsorption kinetics, large capacity, good selectivity ReO4–. Their...

We report a facile and general strategy for enhancing the photostability of organic fluorophores bioimaging applications. As proof concept, bright robust fluorescence was observed in solid states well-defined synthetic polymer polycaprolactone consisting di(thiophene-2-yl)-diketopyrrolopyrrole covalently linked middle chain as biocompatible bioresorbable matrix. The nanoparticles prepared through nanoprecipitation process these polymers could be internalized by both tumor cells stem with...

This article describes molecular design, synthesis and characterization of colloidal nanoparticles containing polycaprolactone-grafted conjugated polymers that exhibit strong far red/near-infrared (FR/NIR) fluorescence for bioimaging. Specifically, we synthesized two kinds polymer bottle brushes (PFTBout-g-PCL PFTBin-g-PCL) with different positions the hexyl groups on thiophene rings. A synthetic amphiphilic block copolymer PCL-b-POEGMA was employed as surfactants to encapsulate PFTB-g-PCL...

Abstract Anisotropic functional patterned surfaces have shown significant applications in microfluidics, biomedicine and optoelectronics. However, surface patterning relies heavily on high-end apparatuses expensive moulds/masks photoresists. Decomposition behaviors of polymers been widely studied material science, but as-created chemical physical structural changes rarely considered as an opportunity for wettability manipulation. Here, a facile mask-free confined-etching strategy is reported...

Abstract Superstructures with complex hierarchical spatial configurations exhibit broader structural depth than single structures and the associated application prospects. However, current preparation methods are greatly constrained by cumbersome steps harsh conditions. Here, for first time, a concise efficient thermally responsive dynamic synthesis strategy of multidimensional superstructures within soluble covalent organic networks (SCONs) tunable morphology from 0D hollow supraparticles...

Abstract Background Emerging evidence suggests that the gut microbiota is closely related to psychiatric disorders. However, little known about role of in development obsessive- compulsive disorder (OCD). Aims & Objectives Here, investigate contribution pathogenesis OCD, we transplanted fecal from first-episode, drug-naive OCD patients or demographically matched healthy individuals into antibiotic-treated specific pathogen-free (SPF) mice. Method Results Our study showed colonization...

Abstract Background Emerging evidence suggests that the gut microbiota is closely related to psychiatric disorders. However, little known about role of in development obsessive- compulsive disorder (OCD). Aims & Objectives Here, investigate contribution pathogenesis OCD, we transplanted fecal from first-episode, drug-naive OCD patients or demographically matched healthy individuals into antibiotic-treated specific pathogen-free (SPF) mice. Method Results Our study showed colonization...

Modulating the reaction reversibility can help escape kinetic traps and effectively regulate morphology of covalent organic frameworks (COFs). However, most current approaches focus primarily on thermodynamic factors with limited research enhancing pathways. Herein, we introduce a novel strategy that combines "freezing–thawing" pretreatment conventional solvothermal synthesis to enhance pathways in COF-366 synthesis. This limits degree monomer polymerization, facilitating structural repairs...

The performance of biofilm-based bioprocesses is difficult to predict and control because the intrinsic heterogeneous dynamic properties microbial biofilms. Biofilm mimics, such as cells entrapped in polymeric scaffolds that are permeable for nutrients, have been proposed replace real biofilms achieve long-term robust engineering applications. However, physiological differences between physically a synthetic matrix biofilm encased self-produced remain unknown. In this study, using Shewanella...

The human gut microbiome (GM) in healthy people is chronically exposed to tetracycline (TET) via environmental exposure and dietary uptake. However, limited information available on its effect the GM metabolome host, especially at level. Here, we investigated how TET both sub-pharmaceutical levels affects secretome-induced host immune response by studying several representative bacteria. Interestingly, showed a highly species-specific pattern with distinct dose-response relationship. B....

Abstract Background Pseudomonas aeruginosa is a notorious opportunistic pathogen causing various types of biofilm-related infections. Biofilm formation unique microbial strategy that allows P. to survive adverse conditions such as antibiotic treatment and human immune clearance. Results In this study, we experimentally evolved PAO1 biofilms for cyclic in the presence high dose imipenem, enriched hyperbiofilm mutants within six cycles two independent lineages. The competition assay showed can...

Opportunistic pathogens such as Pseudomonas aeruginosa adapt their genomes rapidly during chronic infections. Understanding epigenetic regulation may provide biomarkers for diagnosis and reveal novel regulatory mechanisms. We performed single-molecule real-time sequencing (SMRT-seq) to characterize the methylome of a chronically adapted P. clinical strain, TBCF10839. Two N6-methyladenine (6mA) methylation recognition motifs (RCCANNNNNNNTGAR TRGANNNNNNTGC [modification sites are in bold])...

Pseudomonas aeruginosa is a widespread nosocomial pathogen with significant to cause both severe planktonic acute and biofilm-related chronic infections. Small RNAs (sRNAs) are noncoding regulatory molecules that stabilized by the RNA chaperone Hfq trigger various virulence-related signaling pathways. Here, we identified an Hfq-binding sRNA in P. PAO1, PqsS, which promotes bacterial pathogenicity pseudomonas quinolone signal quorum sensing (pqs QS) system. Specifically, PqsS enhanced...

Abstract The stability of covalent organic frameworks (COFs) is crucial for their applications in demanding environments. However, increasing the COFs often comes with challenges such as higher synthesis difficulty, lower crystal quality, and reduced controllability during synthesis, making it difficult to regulate dimensions morphology, thereby impacting processing shaping stable COFs. Herein, study presents a novel confined polymerization approach guided by hydrogen bonding preassembly...

Humans are exposed to many xenobiotics simultaneously, but little is known about the toxic effects based on chemical-chemical interactions. This study aims at evaluating binary interactions between 13 common environmental organic compounds (resulting in 78 pairs) by observing their cytotoxicity HepG2 cells. Among all of tested pairs, combination flame-retardant triphenyl phosphate (TPP) and tris(1,3-dichloro-2-propyl)phosphate (TDCPP) exhibited one most significant synergistic effects. We...