A5033385496- Gut microbiota and health
- Probiotics and Fermented Foods
- Bone and Dental Protein Studies
- Pharmacogenetics and Drug Metabolism
- Asthma and respiratory diseases
- Cell Adhesion Molecules Research
- Neurological disorders and treatments
- Blood properties and coagulation
- Pharmacological Effects and Toxicity Studies
- Muscle activation and electromyography studies
- Dermatology and Skin Diseases
- Biochemical Analysis and Sensing Techniques
- Diet and metabolism studies
- Parkinson's Disease Mechanisms and Treatments
- Drug Transport and Resistance Mechanisms
- Enzyme Production and Characterization
- Olfactory and Sensory Function Studies
- Pharmacological Effects of Natural Compounds
- Drug-Induced Hepatotoxicity and Protection
- Hearing Loss and Rehabilitation
- Garlic and Onion Studies
- Tannin, Tannase and Anticancer Activities
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Multisensory perception and integration
- Ophthalmology and Visual Impairment Studies
Hiroshima University Hospital
2024-2025
Hiroshima University
2010-2023
Seirei Hamamatsu General Hospital
2023
Yazaki (United States)
2023
Nutrition Sciences (Belgium)
2010
University of Florida
2001
We evaluated the effect of an oral administration a plant-derived lactic acid bacterium, Pediococcus pentosaceus LP28 (LP28), on metabolic syndrome by using high fat diet-induced obese mice. The mice were divided into 2 groups and fed either or regular diet for 8 weeks. Each group was further 3 groups, which took LP28, another Lactobacillus plantarum SN13T (SN13T) no bacteria (LAB). lean control without inducing obesity prior to experiment. reduced body weight gain liver lipid contents...
The combination of 5-aminolevulinic acid (5-ALA) phosphate and sodium ferrous citrate (SFC) has been approved as an ingredient in dietary supplements several countries, owing to its broad applicability healthcare. This study aimed assess the safety oral administration 5-ALA SFC healthy adults at doses times higher than those commercially available. included 22 subjects (11 males 11 females) aged 21–59. Doses 250 mg 143.4 (15 Fe) per day were administered orally for 28 days. Blood tests,...
Osteopontin (OPN) is a cytokine and ligand for multiple members of the integrin family. OPN undergoes in vivo polymerization catalyzed by cross-linking enzyme transglutaminase 2, which consequently increases bioactivity through enhanced interaction with integrins. The alpha9beta1, highly expressed on neutrophils, binds to sequence SVVYGLR only after intact cleaved thrombin. appears be cryptic because alpha9beta1 does not recognize OPN. Because 2-catalyzed polymers change their physical...
Osteopontin is a multifunctional glycoprotein with roles in immunomodulation, inflammatory response, tissue mineralization, and remodeling, which are mediated primarily through integrins. Transglutaminase 2 selectively cross‐links proteins by isopeptide bonding. one of the substrates this enzyme undergoes polymerization; however, biological meaning polymerization remains unknown. Using recombinant osteopontin polymerized purified transglutaminase 2, we examined cell adhesion, spreading,...
Abstract Although large populations feel fatigue, the standardized medicinal therapy is currently absent. In this study, we determined whether 5-aminolevulinic acid (5-ALA) supplementation alleviates feeling of fatigue in healthy subjects who chronic physical tiredness. Males and females between ages 20 64 felt on a daily basis, with visual analogue scale (VAS) for ≥ 40 mm, T-score Fatigue-Inertia Profile Mood States—Second Edition—Adult (POMS2-A) 50, Vigor-Activity POMS2-A ≤ 60 were...
The dose-dependent first-pass metabolism of midazolam, a cytochrome P450 (CYP) 3A substrate, was separately estimated in the intestine and liver after administration into jejunal loop rats with differently modulated enzyme activity. Modulation CYP3A activity Sprague-Dawley performed by pretreating inducers such as dexamethasone or co-administering ketoconazole (an inhibitor) midazolam. Bioavailabilities midazolam administered at dose 10 micromol were 12% untreated (control) rats, 2%...
Tannin acylhydrolase (EC 3.1.1.20) referred commonly as tannase catalyzes the hydrolysis of galloyl ester bond tannins to release gallic acid. Although enzyme is useful for various industries, tertiary structure not yet determined. In this study, we determined crystal produced by Lactobacillus plantarum. The belongs a member α/β-hydrolase superfamily with an additional "lid" domain. A glycerol molecule derived from cryoprotectant solution was accommodated into active site. binding manner...
Some glycosides, which are detected in water extracts from medicinal plants, have been reported to be degraded into their aglycones by incubating with some microorganisms producing β-glucosidase. We shown that a plant-derived Lactobacillus plantarum SN13T harbors 11 open reading frames (ORFs) encoding the β-glucosidase enzyme and can grow vigorously several herbal extracts. In this study, we observed extract Artemisia princeps Pampanini (AP) fermented strain strongly inhibited release of...
Some lactic acid bacteria (LAB) are known as representative of probiotics. To screen LAB effective to enhance intestinal immunity, in the present study, we developed an accurate and convenient vitro evaluation system using Peyer's patch cells (PP-cells) isolated from mice intestine. We observed that amount immunoglobulin A (IgA) produced by PP-cells co-cultured with was well correlative PP-cells, intestine feces live after oral administration [correlation coefficient (r)=0.888, 0.883, 0.920,...
A plant‐derived Enterococcus mundtii 15‐1A, that has been previously isolated from Brassica rapa L. subsp. nipposinica (L.H. Bailey) Hanelt var. linearifolia by our group, possesses two kinds of l ‐lactate dehydrogenase ( ‐LDH): LDH‐1 and LDH‐2. was activated under low concentration fluctose‐1,6‐bisphosphate (FBP) at both pH 5.5 7.5. Although LDH‐2 also the FBP 5.5, a high is necessary to activate it The present study shows crystal structures acidophilic in complex with without NADH....
In the present study, we isolated a lactic acid bacterium (LAB) from citrus iyo fruit and identified it as Lactobacillus brevis. This plant-derived LAB strain, designated 174A, produces bacteriocin consisting of two polypeptides brevicin 174A-β 174A-γ. Although each polypeptide itself displays antibacterial activity, ability is enhanced 100 fold by mixing both at 1 : ratio. Significantly, 174A inhibits even growth several pathogenic bacteria that are more resistant to lantibiotic...
The liver dysfunction is a worldwide health problem. It has been suggested that an imbalance of gut microbiome associated with variety diseases. goal the present clinical trial to evaluate effectiveness Lactobacillus plantarum SN13T on hepatic function and fecal microbiota. This study enrolled 22 subjects, aged between 20 70 years, who had any following conditions: 40<AST<100 U/L, 40<ALT<100 or 70<γ-GTP<210 U/L for males; 30<γ-GTP<90 females. subjects were assigned live heat-killed group....
We have recently demonstrated that the exopolysaccharides (EPSs) produced by a plant-derived lactic acid bacterium, Lactobacillus paracasei IJH-SONE68, prevent and ameliorate allergic reaction on contact in dermatitis model mice. In present study, we conducted clinical trial using capsule containing spray-dried powder from pineapple juice broth fermented with LAB strain as an experimental diet. The was double-blind placebo-controlled randomized comparative study May 2019 to July 2021. Males...
The metabolic clearance of midazolam, a cytochrome P450 (CYP) 3A substrate, by the liver under normal and increased enzyme activity in rats was determined in-vivo in-vitro to elucidate reproducibility hepatic extraction ratio midazolam from study. modified pretreating with CYP inducer such as dexamethasone clotrimazole. (ERh,obs) steady-state plasma concentration (approx. 3 nmolmL(-1)) untreated (control) 0.864. This value 0.984 dexamethasone-pretreated 0.964 clotrimazole-pretreated rats....
Whole genome scan analyses have revealed that the chromosomal region 3p21.3, which contains a gene cluster of CC chemokine receptor, is possibly critical for pathogenesis allergic inflammation. Japanese cedar pollinosis mediated by type I allergy and induces seasonal rhinitis conjunctivitis in humans as most common form hay fever spring Japan, although candidate genes remain to be elucidated. We sequenced CCR1, CCR2, CCR3, CCR5, CCXCR1 using PCR restriction fragment length polymorphism...
We tested the repeatability of a finger tapping device with magnetic sensors to determine its reliability. This device, which was developed assist in diagnosis movement disorders such as Parkinson's disease (PD) and strokes, used measure distance between thumb index fingers during movements (repeatedly opening closing gap forefinger). evaluated three types based on interclass correlation coefficient (ICC) analysis variance (ANOVA), i.e., when were measured at different times, that devices,...