Plastic particle pollution poses an emerging threat to ecological and human health. Laboratory animal studies have illustrated that nano-sized plastics can accumulate in the testis cause testosterone deficiency spermatogenic impairment. In this study, TM3 mouse Leydig cells were vitro exposed polystyrene nanoparticles (PS-NPs, size 20 nm) at dosages of 50, 100 150 μg/mL investigate their cytotoxicity. Our results demonstrated PS-NPs be internalized into led a concentration-dependent decline...

Nanoplastics (NPs) have recently emerged in the context of global plastic pollution. They may be more toxic than macroplastics litter and microplastic fragments due to its abundances, tiny sizes, cellular accessibility. The female reproductive toxicity NPs has been widely documented for aquatic animals, but their effects underlying mechanisms remain poorly understood mammals. This study aimed explore on reproduction using human ovarian granulosa cells (GCs) mice. accumulation polystyrene...

Premature ovarian insufficiency (POI) is a clinical syndrome of dysfunction characterized by the abnormal alteration hormone levels such as FSH and E2. POI causes infertility, severe daily life disturbances, long-term health risks. However, underlying mechanism remains largely unknown. In this study, we found that associated with cellular senescence granulosa cells, TRIM28 mediates oxidative stress (OS)-induced in cells. Mechanistically, OS decrease protein KGN Subsequently, it triggers an...

Background: The Hippo signaling pathway, a highly conserved cell system, exists in most multicellular organisms and regulates proliferation, differentiation, apoptosis. It has been reported that the members of are expressed mammalian ovaries, but exact functions this pathway primordial follicle development remains unclear. Methods: To analyze spatio-temporal correlation between core component size pool, Western blot, Real-time PCR immunohistochemistry were used, expression localization MST1,...

Clarifying the molecular mechanisms by which primordial follicles are initiated is crucial for prevention and treatment of female infertility ovarian dysfunction. The Hippo pathway has been proven to have a spatiotemporal correlation with size follicle pool in mice our previous work. But role underlying activation remain unclear. Here, localization expression core components were examined before after activation. And effects on determined genetically manipulating yes-associated protein 1...

This study was designed to investigate the protective effect of resveratrol (RES) on premature ovarian failure (POF) and proliferation female germline stem cells (FGSCs) at tissue cell levels. POF mice were lavaged with RES, ovaries co-cultured RES and/or GANT61 in vitro. FGSCs pretreated Busulfan M1 macrophages, which RES. The weights their ovaries, as well follicle number, measured. Ovarian function, antioxidative stress, inflammation, survival evaluated. significantly increased number...

Objective: To improve the separation, identification and cultivation of ovarian germline stem cells (OGSCs), to clarify relationship between Hippo signaling pathway effector YAP1 proliferation differentiation OGSCs in vitro identify major contribution function. Methods: Two-step enzymatic separation processes magnetic were used isolate by determining expression Mvh, Oct4, Nanog, Fragilis Stella markers. Then, YAP1, as main molecule pathway, was chosen target gene study. Lentivirus containing...

Nanoplastic contamination has been of intense concern by virtue the potential threat to human and ecosystem health. Animal experiments have indicated that exposure nanoplastics (NPs) can deposit in liver contribute hepatic injury. To explore mechanisms hepatotoxicity induced polystyrene-NPs (PS-NPs), mice AML-12 hepatocytes were exposed different dosages 20 nm PS-NPs this study. The results illustrated vitro vivo triggered excessive production reactive oxygen species repressed nuclear factor...

Ovarian aging is a process of decline in its reserve leading to ovary dysfunction and even reduced health quality offspring. However, aging-related molecular pathways the remain obscure. Lysine succinylation (Ksuc), newly post-translational modification (PTM), has been found be broadly conserved both eukaryotic prokaryotic cells, associated with multiple pathophysiological processes. There are no relevant reports revealing link between mechanisms ovarian Ksuc.The level Ksuc ovaries aged...

The Hippo signaling pathway plays fundamental roles in stem cell maintenance a variety of tissues and has thus implications for biology. Key components this recently discovered have been shown to be associated with primordial follicle activation. However, whether the role development Ovarian Germline Stem Cells (OGSCs) during physiological pathological ovarian aging mice is unknown.Mice at age 7 days (7D), or 2, 10, 20 months (2M, 10M, 20M) 2M treated TPT CY/BUS drugs were selected as...

Hematopoiesis is maintained throughout life by self-renewing hematopoietic stem cells (HSCs) that differentiate to produce both myeloid and lymphoid cells. The NR4A family of orphan nuclear receptors, which regulates cell fate in many tissues, appears play a key role HSC proliferation differentiation. Using NR4A1(GFP) BAC transgenic reporter mouse we have investigated NR4A1 expression its regulation early hematopoiesis. We show most highly expressed subset Lin(-) Sca-1(+) c-Kit(+) CD48(-)...

Abstract Background C-src is an evolutionarily conserved proto-oncogene that regulates cell proliferation, differentiation and apoptosis. In our previous studies, we have reported another proto-oncogene, c-erbB 2 , plays important role in primordial follicle activation development. We also found c-src was expressed mammalian ovaries, but its functions remain unclear. The objective of this study to investigate the mechanism during growth follicles. Methods Ovaries from 2-day-old rats were...

Abstract Background c-erbB2, a proto-oncogene coding epidermal growth factor receptor-like receptor, also as chemosensitivity/prognosis marker for gynecologic cancer, may be involved in initiation of rat primordial follicles. The aim the present study is to investigate role and signal pathway c-erbB2 onset follicle development. Methods expression mRNA protein neonatal ovaries cultured 4 8 days with/without (EGF) were examined by situ hybridization, RT-PCR western blot. function...

Background/Aims: Ovarian germline stem cells (OGSCs) have been shown to mainly exist in the ovarian surface epithelium (OSE), but activity changes of during different reproductive stages and potential regulatory signaling pathway are still unknown. The Notch plays a key role cell development, primordial follicles proliferation. However, whether it proliferation OGSCs is Here, we analyzed correlation between pathway. Methods: expression markers Mvh, Ooc4 molecules Notch1, Hes1, Hes5 were...

Proto-oncogenes are involved in cell growth, proliferation, and differentiation. In the present study, we investigated roles mediating pathways of proto-oncogenes c-erbB2 c-myb mouse oocyte maturation by RT-PCR, real-time quantitative PCR, western blot, recombinant proto-oncogene protein microinjection. Results showed that both antisense oligodeoxynucleotides (c-erbB2 ASODN ASODN) inhibited germinal vesicle breakdown first polar body extrusion a dose-dependent manner. However, microinjection...

Pten (phosphatase and tensin homolog deleted on chromosome 10), a kind of tumor suppressor gene, plays important roles in female reproductive system. But its expression the formation polycystic ovaries are yet to be known. In this study, we constructed rat model PCOS using norethindrone HCG injections found expressions pten mRNA PTEN protein increased significantly ovary tissue by immunohistochemistry, RT-PCR, western blot. Furthermore, results showed that vivo could effectively transfected...

PAQR7 plays a key role in cell apoptosis as progesterone membrane receptor. The physiological mechanism of ovarian function and its anti-apoptotic action mammals remain poorly understood. We first added 0.2 µM aminoglutethimide (AG), an inhibitor endogenous (P4) secretion, transfected siPAQR7 co-incubated with P4 human KGN cells to identify granulosa apoptosis, respectively. Additionally, we used Paqr7 knockout (PAQR7 KO) mice assess the ovary. deficiency significantly increased cells, this...