Abstract Background Bone metastasis (BM) is one of the common sites hepatocellular carcinoma (HCC), and prognosis BM patients worse than without it. Our study aimed to identify predictors prognostic factors in HCC develop two nomograms quantify risk with BM. Methods We retrospectively reviewed data who were diagnosed as between 2010 2015 Surveillance, Epidemiology, End Results (SEER) database. Independent for from determined by univariate multivariate logistic regression analysis. identified...

Objective: To improve the separation, identification and cultivation of ovarian germline stem cells (OGSCs), to clarify relationship between Hippo signaling pathway effector YAP1 proliferation differentiation OGSCs in vitro identify major contribution function. Methods: Two-step enzymatic separation processes magnetic were used isolate by determining expression Mvh, Oct4, Nanog, Fragilis Stella markers. Then, YAP1, as main molecule pathway, was chosen target gene study. Lentivirus containing...

Gastric cancer (GC) is one of the most common malignancies worldwide, exhibiting a high morbidity, and mortality. As various treatment methods for gastric are limited by disadvantages, many efforts to improve efficacy these treatments being taken. Metabolic recombination an important characteristic has gradually caused recent upsurge in research. However, systematic analysis interaction between glycolysis GC patient prognosis its potential associations with immune infiltration lacking but...

Non-small-cell lung cancer (NSCLC) patients often develop bone metastases (BM), and the overall survival for these is usually perishing. However, a model with high accuracy predicting of NSCLC BM still lacking. Here, we aimed to establish based on artificial intelligence 1-year rate by using extreme gradient boosting (XGBoost), large-scale machine learning algorithm. We selected between 2010 2015 from Surveillance, Epidemiology, End Results database. In total, 5973 cases were enrolled...

Abstract Background Breast cancer is the most common malignancy in women, and it also leading cause of death female patients; pathological type BC infiltrating duct carcinoma (IDC). Some nomograms have been developed to predict bone metastasis (BM) patients with breast cancer. However, there are no studies on diagnostic prognostic for BM newly diagnosed IDC patients. Methods from 2010 2016 Surveillance, Epidemiology End Results (SEER) database were reviewed. Multivariate logistic regression...

Oocyte maturation disorder and decreased quality are the main causes of infertility in women, granulosa cells (GCs) provide only microenvironment for oocyte through autocrine paracrine signaling by steroid hormones growth factors. However, chronic inflammation oxidative stress caused ovarian hypoxia largest contributors to aging GC dysfunction. Therefore, amelioration is expected be a pivotal method improve function quality. In this study, we detected protective effect chitosan...

Abstract Background Tumor microenvironment (TME) plays an important role in malignant tumors. Our study aimed to investigate the effect of TME and related genes osteosarcoma patients. Methods Gene expression profiles clinical data patients were downloaded from TARGET dataset. ESTIMATE algorithm was used quantify immune score. Then, association between score prognosis studied. Afterward, a differential analysis performed based on high- low-immune scores determine TME-related genes....

Background Total joint arthroplasty (TJA), including total knee (TKA) and hip (THA), is required for many patients. This study aimed to evaluate the medical costs, length of stay (LOS), blood transfusion in-hospital complications in patients undergoing simultaneous staged TJA. Methods All who underwent primary bilateral TJA from 2013 2018 our institute were included. The propensity score matching analysis was performed between difference LOS, compared groups. Results Except materials fees...

Intervertebral disc degeneration (IDD) is a prevalent degenerative disorder that closely linked to aging.Numerous studies have indicated the crucial involvement of autophagy in development IDD.However, non-selective nature substrates poses great limitations on application autophagy-related medications.This study aims enhance our comprehension IDD and investigate novel therapeutic approach from perspective selective receptor NBR1.Proteomics immunoprecipitation mass spectrometry analysis,...

Background: The global COVID-19 epidemic remains severe, with the cumulative death toll reaching more than 207,170 as of May 2, 2020 (1). Purpose: Our research objective is to establish a reliable nomogram predict mortality in patients. can help us distinguish between patients who are at high risk and need close attention. Patients Methods: For single-center retrospective study, we collected 21 cases died critical illness area Optical Valley Branch Tongji Hospital, Huazhong University...

Endometrial cancer (EC) is one of the most common gynecological cancers. Epithelial-mesenchymal transition (EMT) believed to be significantly associated with malignant progression tumors. However, there no relevant study on relationship between EMT-related gene (ERG) signatures and prognosis EC patients.We extracted mRNA expression profiles 543 tumor 23 normal tissues from The Cancer Genome Atlas database. Then, we selected differentially expressed ERGs (DEERGs) among these mRNAs. Next,...

Among colon cancer patients, liver metastasis is a commonly deadly phenomenon, but there are few prognostic models for these patients.The clinicopathologic data of with (CCLM) patients were downloaded from the Surveillance, Epidemiology and End Results (SEER) database. All randomly divided into training internal validation sets based on ratio 7:3. A nomogram was established Cox analysis in set, which validated by two independent sets.A total 5,700 CCLM included. Age, race, tumor size, site,...

Abstract Background Cervical cancer (CC) is one of the most common gynaecological cancers. The gene signature believed to be reliable for predicting patient survival. However, there no relevant study on relationship between glycolysis-related (GRG) and overall survival (OS) patients with CC. Methods We extracted mRNA expression profiles 306 tumour 13 normal tissues from University California Santa Cruz (UCSC) Database. Then, we screened out differentially expressed genes (DEGRGs) among these...

Abstract Background Percutaneous endoscopic cervical decompression (PECD) is an ideal minimally invasive technique for the treatment of spondylotic radiculopathy (CSR). However, mainstream resection dorsal bone and removal free nucleus pulposus. The necessity excision ventral osteophytes hyperplastic ligaments in CSR caused by foraminal and/or lateral spinal stenosis (CFa/oLSS) to be discussed. Methods We performed a retrospective study 46 patients with CFa/oLSS from January 2017 November...

Patients with non-small-cell lung cancer (NSCLC) often have a poor prognosis when brain metastases (BM) occur. This study aimed to evaluate the prognostic factors of BM in newly diagnosed NSCLC patients and construct nomogram predict overall survival (OS). We included from 2010 2015 Surveillance, Epidemiology, End Results database. The independent for were determined by Cox proportional hazards regression analysis. then constructed validated OS BM. finally 4129 Age, race, sex, liver...

Abstract Background Extremity liposarcoma represents 25% of extremity soft tissue sarcoma and has a better prognosis than occurring in other anatomic sites. The purpose this study was to develop two nomograms for predicting the overall survival (OS) cancer-specific (CSS) patients with liposarcoma. Methods A total 2170 diagnosed primary between 2004 2015 were extracted from Surveillance, Epidemiology, End Results (SEER) database. Univariate multivariate Cox analyses performed explore...

Abstract Background Triple-negative breast cancer (TNBC) is widely concerning because of high malignancy and poor prognosis. There increasing evidence that alternative splicing (AS) plays an important role in the development formation tumour microenvironment. However, comprehensive analysis AS signalling TNBC still lacking urgently needed. Methods Transcriptome clinical data 169 tissues 15 normal were obtained integrated from genome atlas (TCGA), overview events was downloaded SpliceSeq...

Abstract Background The aim of this study was to develop and validate a visual nomogram for predicting the risk bone metastasis (BM) in newly diagnosed thyroid carcinoma ( TC) patients. Methods demographics clinicopathologic variables TC patients from 2010 2015 Surveillance, Epidemiology End Results (SEER) database were retrospectively reviewed. Chi - squared (χ2) test logistic regression analysis performed identify independent factors. Based on that, predictive developed validated BM...

Kidney renal clear cell carcinoma (KIRC) is the leading cause of kidney cancer-related deaths. Currently, there are no studies in tumor immunology investigating use signatures as a predictor overall survival KIRC patients. Our study attempts to establish an immune-related gene risk signature predict clinical outcomes KIRC. A total 528 patients from The Cancer Genome Atlas (TCGA) database were included our analysis and randomly divided into training ( n = 315) testing sets 213). We collected...

Overall survival (OS) and cancer-specific (CSS) of luminal A breast cancer (BC) patients with bone metastasis remain poor vary dramatically from person to person. Our goal was build two universally applicable nomograms accurately predict OS CSS for metastasis.

Background: The expression of long non-coding RNA (lncRNA) is associated with the epithelial-mesenchymal transition (EMT) in tumorigenicity, but role EMT-related lncRNA colorectal cancer (CRC) remains unclear. Methods: clinical data and gene profile CRC patients were obtained from Cancer Genome Atlas database. Differential analysis, Cox regression model, Kaplan-Meier analysis used to study relationship between lncRNAs prognosis CRC. Functional unsupervised clustering performed explore...