A5083049964- Microbial Natural Products and Biosynthesis
- Synthetic Organic Chemistry Methods
- X-ray Diffraction in Crystallography
- Plant-based Medicinal Research
- Crystallization and Solubility Studies
- Alkaloids: synthesis and pharmacology
- Bioactive Natural Diterpenoids Research
- Marine Sponges and Natural Products
- Carbohydrate Chemistry and Synthesis
- Synthesis of Organic Compounds
- Chemical Synthesis and Analysis
- Traditional and Medicinal Uses of Annonaceae
- Natural product bioactivities and synthesis
- Phytochemical compounds biological activities
- Synthesis and Catalytic Reactions
- Bioactive Compounds and Antitumor Agents
- Fungal Plant Pathogen Control
- Cancer therapeutics and mechanisms
- Plant-derived Lignans Synthesis and Bioactivity
- Plant Pathogens and Fungal Diseases
- Fungal Biology and Applications
- Catalytic C–H Functionalization Methods
- Intermetallics and Advanced Alloy Properties
- Plant Disease Resistance and Genetics
- Antimicrobial Resistance in Staphylococcus
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2024
National Health and Family Planning Commission
2016
Academia Sinica
1993
Institute of Metallurgy
1993
Seventeen new alkaloids (1-17) and 14 known analogues have been isolated from an aqueous extract of the root Isatis indigotica. The structures absolute configurations these compounds were determined by extensive spectroscopic data analysis, including 2D NMR, single-crystal X-ray crystallography using anomalous scattering Cu Kα radiation, electronic circular dichroism spectra calculations based on quantum-mechanical time-dependent density functional theory. Compounds 1, 2, 3 are first...
A pair of enantiomers (1a and 1b) an indole alkaloid containing dihydrothiopyran 1,2,4-thiadiazole rings was isolated from aqueous extract the root Isatis indigotica. The structures absolute configurations were determined by extensive spectroscopic analysis, especially 2D NMR, modified Mosher's method, electronic CD (ECD). proposed biosynthetic pathway preliminary investigations biological activity compounds 1a 1b against influenza virus A/Hanfang/359/95 (H3N2) HSV-1 are also discussed.
Twenty-six new diterpenoid alkaloids, 1-26 (1-4: hetisan-type C(20)-diterpenoid alkaloids; 5-26: aconitane C(19)-diterpenoid alkaloids), and two known analogues, hypaconitine 27 benzoylmesaconine 28, have been isolated from a water extract of the lateral root Aconitum carmichaelii. Compounds 7 8 are rare examples conformational isomers obtained same material. The conformation transformation ring A in alkaloids discussed on basis NMR data analysis combination with single-crystal X-ray...
Microbial sulfur-containing secondary metabolites show various biological activities, but the C-S bond-forming in their biosynthetic metabolism has not been thoroughly understood. Here, we present genetic, biochemical and structural characterization of a cytochrome P450 monooxygenase CxnD exhibiting bond forming activity S-heterocyclization chuangxinmycin biosynthesis. In vivo vitro analyses demonstrated that generated an indole-fused dihydrothiopyran skeleton from L-Trp-derived thiol...
Seven new aromatic acid derivatives (1–7), together with five known analogs, were isolated from the lateral roots of Aconitum carmichaelii. Structures compounds determined by spectroscopic and chemical methods as 4-methyl ( − )-(R)-hydroxyeucomate (1), 4-butyl (2), 4-butyl-1-methyl (+)-(R)-2-O-(4′-hydroxy-3′-methoxybenzoyl)malate (3), 1-butyl-4-methyl (4), dimethyl (5), (+)-(R)-2-O-(4′-hydroxybenzoyl)malate (6), methyl ± )-3-(4′-hydroxy-3′-methoxyphenyl)-3-sulfopropionate (7), respectively....
Summary The control of secondary production in streptomycetes involves the funneling environmental and physiological signals to cluster‐situated (transcriptional) regulators ( CSRs ) biosynthetic genes. For some systems, binding products CSR has been shown provide negative feedback. Here we show for lidamycin C ‐1027), a clinically relevant antitumor agent, by S treptomyces globisporus that feedback can extend point higher regulatory cascade. We DNA ‐binding activity . orthologue AtrA ,...
A hybrid trans-AT PKS/NRPS gene cluster htm was identified with defined boundaries for hangtaimycin biosynthesis in Streptomyces spectabilis CPCC200148. Deoxyhangtaimycin, a new derivative of hangtaimycin, from the htm11 knockout mutant. In vitro biochemical assays demonstrated that cytochrome P450 monooxygenase Htm11 responsible stereoselective hydroxylation deoxyhangtaimycin to hangtaimycin. More importantly, showed activity against influenza virus at micromolar level, highlighting its...
AntiSMASH analysis of genome DNA Streptomyces CPCC 204980, a soil isolate with potent antibacterial activity, revealed gene cluster for polycyclic xanthones. A subsequent chemical study confirmed that the microorganism produced xanthone cervinomycin A2 (1) and new congeners cervinomycins B1–4 (2–5). The structures 1–5 were determined by comprehensive analyses MS NMR data, which indicated 2–5 featured common dihydro-D ring in core moiety their molecules. are toxic to human cancer cells active...
Three dimeric analogues of bohemamines, dibohemamines D–F (1–3), together with dibohemamine A (4), were isolated from Streptomyces sp. CPCC 200497. Their structures solved using a combination mass spectrometry, 1D and 2D NMR spectroscopy, CD. Dibohemamines D E new F was known compound obtained previously by semisynthesis. Dibohemamine displayed potent cytotoxicity against cancer cell lines A549 HepG2 IC50 values 1.1 0.3 μM, respectively. showed moderate HepG2.
Isatropolone C from Streptomyces sp. CPCC 204095 features a fused cyclopentadienone-tropolone-oxacyclohexadiene tricyclic moiety in its structure. Herein, we report an isatropolone dimer derivative, di-isatropolone C, formed spontaneously methanol. Notably, the structure of resolved by NMR reveals newly cyclopentane ring to associate two monomers. The configurations four chiral carbons, including ketal one, are assigned using quantum calculations and DP4+ probability. plausible molecular...
Actinosynnema is a small but well-known genus of actinomycetes for production ansamitocin, the payload component antibody-drug conjugates against cancers. However, secondary metabolite profile pretiosum ATCC 31565, most famous producer has never been fully explored. Our antiSMASH analysis genomic DNA 31565 revealed NRPS-PKS gene cluster polyene macrolactam. The very similar to clusters mirilactam and salinilactam, two 26-membered macrolactams from mirum Salinispora tropica, respectively....
Isarubrolones are bioactive polycyclic tropoloalkaloids from Streptomyces. Three new isarubrolones (2-4), together with the known isarubrolone C (1) and isatropolones A (5) (6, 3( R)-hydroxyisatropolone A), were identified Streptomyces sp. CPCC 204095. The structures of these compounds determined using a combination mass spectrometry, 1D 2D NMR spectroscopy, ECD. Compounds 3 4 feature pyridooxazinium unit, which is rarely seen in natural products. Compound 6 could conjugate amino acids or...
A new congener of chuangxinmycin (CM) was identified from Actinoplanes tsinanensis CPCC 200056. Its structure determined as 3-methylchuangxinmycin (MCM) by 1D and 2D NMR. MCM could be generated in vivo CM heterologous expression the vitamin B12-dependent radical SAM enzyme CxnA/A1 responsible for methylation 3-demethylchuangxinmycin (DCM) biosynthesis, indicating that perform iterative production. In vitro assays revealed significant activities CM, DCM, against Mycobacterium tuberculosis...
A new granaticin analogue and its hydrolysis product were isolated from Streptomyces sp. CPCC 200532. Their structures determined to be 6-deoxy-13-hydroxy-8,11-dione-dihydrogranaticins B (1) (2), respectively, by detailed analysis of spectroscopic data. Compound 1 was regarded as an intermediate in biosynthesis, it bioconvertable B. Compared B, showed similar cytotoxicity against cancer cell line HCT116, but decreased lines A549, HeLa, HepG2. 2 displayed lower than all four tested.
A novel natural geldanamycin analogue was discovered in Streptomyces hygroscopicus 17997. Its 4,5-dihydro form also identified the gdmP gene disruption mutant of The structures two compounds were determined to be 19-[(1′S,4′R)-4′-hydroxy-1′-methoxy-2′-oxopentyl]geldanamycin (1) and 19-[(1′S,4′R)-4′-hydroxy-1′-methoxy-2′-oxopentyl]-4,5-dihydrogeldanamycin (2), respectively, by extensive spectroscopic data analysis, including 2D NMR, modified Mosher's method, electronic circular dichroism....
Genomic analysis indicated that the genomes of ascomycetes might carry dozens biosynthetic gene clusters (BGCs), yet many have remained enigmatic. The ascomycete genus Epicoccum, belonging to family Didymellaceae, is ubiquitous colonizes different types substrates and associated with phyllosphere or decaying vegetation. Species this are prolific producers bioactive substances. epicoccamides, as biosynthetically distinct mannosylated tetramate, were first isolated in 2003 from Epicoccum sp....