A5013025880- Axon Guidance and Neuronal Signaling
- Zebrafish Biomedical Research Applications
- Neurogenesis and neuroplasticity mechanisms
- Angiogenesis and VEGF in Cancer
- Advanced Vision and Imaging
- Neurobiology and Insect Physiology Research
- Human Pose and Action Recognition
- Neural dynamics and brain function
- Cellular transport and secretion
- Retinal Development and Disorders
- Robotic Locomotion and Control
- 3D Surveying and Cultural Heritage
- Primary Care and Health Outcomes
- Video Surveillance and Tracking Methods
- Nanopore and Nanochannel Transport Studies
- Abdominal Surgery and Complications
- Remote Sensing and LiDAR Applications
- Model Reduction and Neural Networks
- Mining Techniques and Economics
- Intestinal and Peritoneal Adhesions
- Cancer Mechanisms and Therapy
- Carbon Nanotubes in Composites
- Functional Brain Connectivity Studies
- Developmental Biology and Gene Regulation
- Caveolin-1 and cellular processes
École Polytechnique Fédérale de Lausanne
2019-2025
McGill University
2013-2024
Hospital Clínico Universitario Virgen de la Victoria
2024
University of Southern Denmark
2022
Genomics (United Kingdom)
2022
University of Dundee
2022
Montreal Clinical Research Institute
2013-2019
Studying how neural circuits orchestrate limbed behaviors requires the precise measurement of positions each appendage in three-dimensional (3D) space. Deep networks can estimate two-dimensional (2D) pose freely behaving and tethered animals. However, unique challenges associated with transforming these 2D measurements into reliable 3D poses have not been addressed for small animals including fly, Drosophila melanogaster. Here, we present DeepFly3D, a software that infers tethered, adult...
During neural circuit assembly, axonal growth cones are exposed to multiple guidance signals at trajectory choice points. While responses individual cues have been extensively studied, less is known about combination of and underlying molecular mechanisms. Here, we studied the convergence directing selection spinal motor axons entering limb. We first demonstrate that Netrin-1 attracts repels distinct axon populations, according their expression Netrin receptors. Quantitative in vitro assays...
Background Multimorbidity poses major challenges to healthcare systems worldwide. Definitions with cut-offs in excess of ≥2 long-term conditions (LTCs) might better capture populations complexity but are not standardised. Aim To examine variation prevalence using different definitions multimorbidity. Design and setting Cross-sectional study 1 168 620 people England. Method Comparison multimorbidity (MM) four definitions: MM2+ (≥2 LTCs), MM3+ (≥3 from 3+ LTCs ≥3 International Classification...
Stochastic long-term mine planning has evolved to account for different sources of uncertainty. Typically, the uncertainty and local variability boundaries in geological domains have been overlooked by experts through their deterministic interpretation available data. Categorical attributes are used model domains, stochastic simulation accounts mentioned issues. The ability two-points methods reproduce complex patterns or requirement a training image multiple-points limited implementation...
Classic studies have proposed that genetically encoded programs and spontaneous activity play complementary but independent roles in the development of neural circuits. Recent evidence, however, suggests these two mechanisms could interact extensively, with affecting expression function guidance molecules at early developmental stages. Here, using developing chick spinal cord mouse visual system to ectopically express inwardly rectifying potassium channel Kir2.1 individual embryonic neurons,...
Abstract The signalling output of many transmembrane receptors that mediate cell-cell communication is restricted by the endosomal sorting complex required for transport (ESCRT), but impact this machinery on Eph tyrosine kinase receptor function unknown. We identified ESCRT-associated adaptor protein HD-PTP as part an EphB2 proximity-dependent biotin identification (BioID) interactome, and confirmed association using co-immunoprecipitation. loss attenuates ephrin-B2:EphB2 signalling-induced...
Extracting the relationship between high-dimensional recordings of neural activity and complex behavior is a ubiquitous problem in systems neuroscience. Toward this goal, encoding decoding models attempt to infer conditional distribution given vice versa, while dimensionality reduction techniques aim extract interpretable low-dimensional representations. Variational autoencoders (VAEs) are flexible deep-learning commonly used embeddings or behavioral data. However, it challenging for VAEs...
Abstract Markerless 3D pose estimation has become an indispensable tool for kinematic studies of laboratory animals. Most current methods recover by multi-view triangulation deep network-based 2D estimates. However, requires multiple, synchronized cameras and elaborate calibration protocols that hinder its widespread adoption in studies. Here, we describe LiftPose3D, a method overcomes these barriers reconstructing poses from single camera view. We illustrate LiftPose3D’s versatility...
The precise assembly of a functional nervous system relies on the guided migration axonal growth cones, which is made possible by signals transmitted to cytoskeleton cell surface-expressed guidance receptors. We investigated function ephexin1, Rho guanine nucleotide exchange factor, as an essential growth-cone intermediary in context spinal lateral motor column (LMC) axon trajectory selection limb mesenchyme. Using situ mRNA detection, we first show that ephexin1 expressed LMC neurons chick...
Abstract Studying how neural circuits orchestrate limbed behaviors requires the precise measurement of positions each appendage in 3-dimensional (3D) space. Deep networks can estimate 2-dimensional (2D) pose freely behaving and tethered animals. However, unique challenges associated with transforming these 2D measurements into reliable 3D poses have not been addressed for small animals including fly, Drosophila melanogaster . Here we present DeepFly3D, a software that infers tethered, adult...
During neural circuit development, attractive or repulsive guidance cue molecules direct growth cones (GCs) to their targets by eliciting cytoskeletal remodeling, which is reflected in morphology. The experimental power of vitro neuronal cultures assay this process and its molecular mechanisms well established, however, a method rapidly find quantify multiple morphological aspects GCs lacking. To end, we have developed free, easy use, fully automated Fiji macro, Conographer, accurately...
Abstract Background Outpatient surgery (OS) is consolidated in many pathologies, but there are still controversies regarding endocrine surgery. The objective of this study was to present the results about OS parathyroid program a reference hospital. Methods patient selection criteria included those patients with diagnosis primary hyperparathyroidism (HPT1°) due adenoma, two positive localization tests, ages between 18 and 80 years, an ASA I, II or III pre-anesthetic assessment, who live less...
Background: A bezoar is defined as a foreign body resulting from the accumulation of ingested material or food in stomach. However, they can also be found duodenum and other segments intestine. The average age occurrence 11 years, predominantly affecting females. Among its complications, gastric outlet obstruction most frequent, though secondary perforation extremely rare. Material Methods: Four clinical cases are presented third‐level care facility Mexico City over period 2022–2024, all...
This paper presents the project of virtual reconstruction and inspection Portalada, entrance Ripoll Monastery. In a first step, monument 7 x 11 meters was acquired using triangulation laser scanning technology, producing dataset more than 2000 range maps for total one billion triangles. After alignment registration, nearly complete digital model with 173M triangles sampling density order millimeter produced repaired. The describes acquisition construction, use specific scalable algorithms...
Abstract To date, the engineering of single-stranded DNA-SWCNT (DNA-SWCNT) optical biosensors have largely focused on creating sensors for new applications with little focus optimising existing in vitro and vivo conditions. Recent studies shown that nanotube fluorescence can be severely impacted by changes local cation concentrations. This is particularly problematic neurotransmitter sensing as spatial temporal fluctuations concentration cations, such Na + , K or Ca 2+ play a central role...